Our study on nanodiscs and ion channel structure is now published: https://t.co/PZ6gvXLIx4. We hope this will spur more consideration into the impact of nanodiscs on membrane protein structure.
@VikramDalal_37 @PageAnesthesia @GraceBrannigan @WUSTL_AnesRsrch
This is a very interesting paper showing that nanodiscs, typically used to preserve lipids for structural analysis of membrane proteins, can affect the structure of the target protein.
First paper I read this year, happy 2024!
https://t.co/cH8ggsfRJY
Another paper from my postdoc with @CrinaNimigean on lipid modulation of an ion channel is out now. Great effort by @thon_oli further showcasing the value of a great model system. Congrats to the entire team!
https://t.co/AcJye0p2dw
I liked the reaction on the soundtrack/musical synopsis for our last paper, maybe every paper should have one. If you haven't seen/heard it yet, weekend is coming !
Paper @embojournal https://t.co/wCzUlhcMKg
Soundtrack by me and byteBBY @BebeByte
https://t.co/1tYtHPBmzS
Structures of channels in Open conformation are rare. Thrilled to share our latest manuscript..
Structural basis for hyperpolarization-dependent opening of human HCN1 channel https://t.co/L10NUeZWyk
A postdoc position is available in our lab to study how ion channels communicate with one another. Cryo-EM, electrophysiology, protein biophysics. A great opportunity for structural biologists to learn electrophysiology methods or vice versa.
https://t.co/FWLrNqu2UK
These simulations of membrane proteins in nanodiscs were so fun. Thank you @WChengLab for your continued enthusiasm for simulations that go beyond the basics - we are lucky computational collaborators! @VikramDalal_37 @PageAnesthesia
@danielmihaylov7@MishaKudryashev Also, there is still some variability in M4 resolution of ELIC structures from other studies using MSP1E3D1 and SMA. See agonist-bound ELIC in MSP1E3D1 (emd-20968).
@danielmihaylov7@MishaKudryashev The impact of M4 interactions with the nanodisc is something we are continuing to work on, so hopefully will have more insights soon.
@danielmihaylov7@MishaKudryashev Yes, M4 is generally not as well resolved but there is density in the unsharpened maps to observe major differences in helix orientation. From the MD simulations, one can also see how the nanodisc can impact the orientation of M4.
@danielmihaylov7@MishaKudryashev Agree with @MishaKudryashev. We focused on the backbone structure of the helices (instead of rotamers) and observed 1-2 angstrom differences, which we believe are significant.
It's my pleasure to share that I have been awarded an AHA postdoctoral fellowship. I would like to acknowledge my mentor @WChengLab for unimaginable support, referres: @Pravindrak_IITR, @ShaillyTomar and Alex Evers, and @American_Heart for belief in my proposal.
@WChengLab