WittbrodtLab

✂️ #CRISPR tool ✂️ #Baseeditor tool ✏

[WittbrodtLab @WittbrodtLab]

LAST CHANCE TO REGISTER! Calling all medaka researchers and enthusiasts!
Don't miss to connect with the growing #medaka community, share ideas & spark new collaborations! The Medaka PI/ERC Indigene Meeting 2025 is just around the corner! REGISTER NOW → https://t.co/5ePr35mMrx

[WittbrodtLab @WittbrodtLab]

new #preprint: mRNA delivered degron system w/ GFP-nanobody to efficiently reduce GFP-tagged proteins in an inducible manner 🐟-Case study: reduction of PMM2 (basis for ER-based #glycosylation) causes hypoglycosylation reported from CDG-patients https://t.co/imYUDoM48U #CRISPR

[WittbrodtLab @WittbrodtLab]

Calling all #medaka PI researchers! Join us in Heidelberg, Germany July 22-24, 2025 for our collaborative meeting! Register now https://t.co/fwL5IuB1xZ & take advantage of early bird rates until April 15th! 🐟 #MedakaResearch #ModelOrganisms #ScienceConference #FishModels #fish

[WittbrodtLab @WittbrodtLab]

check out the new paper from our lab with @KGoepfrich and @SchwarzUlrich groups where we put DNA microbeads for precise signal control in #organoids. https://t.co/fYoK3gyANh @AftingCassian @c_schlagheck @Cluster3DMM2O

[3D Matter Made to Order @Cluster3DMM2O]

@AftingCassian, @ToWalther, @c_schlagheck, @SchwarzUlrich, @JWittbr, @KGoepfrich developed DNA microbeads for precise signal control in #organoids. Published in @NatureNano, this innovation advances tissue modeling & light-controlled signals. 🧬 https://t.co/wHsuhpSwPD #Nanotech

[WittbrodtLab @WittbrodtLab]

New high-throughput optomotor response (OMR) assay for medaka fish larvae reveals strain-specific visual function differences & training effects. Our open-source setup enables studies on visual acuity, color sensitivity & more #NeuroBiology #FishResearch 🐠https://t.co/KV2xZtVRYC

[WittbrodtLab @WittbrodtLab]

fruitful collaboration among the @Cluster3DMM2O with the labs of Eva Blasco @EvaBlascoPo, Kerstin Göpfrich @KGoepfrich and Steffen Lemke

[WittbrodtLab @WittbrodtLab]

New platform enables in vivo 3D laser printing of microimplants using synthetic polymer inks. We showed in fish and fly embryos that this minimal-invasive technique opens doors for direct tissue engineering in various organisms #BioEngineering #3DPrinting https://t.co/VEFUP7n1Oe

[GfE @GfEeV]

That's it: #GfE2024 is over. Thanks everyone for the science, amazing talks, chairing, buzzing poster sessions, short & long discussions, organizers in the back & front - simply thanks for all your contributions! See you all latest at #GfE2026 for updates & new routes of #DevBio

[WittbrodtLab @WittbrodtLab]

Southernblot FTW! #CRISPR #HDR

[Thomas Thumberger @_ThomThum_]

If you desperately need to explain the problem of in vitro recombination in #PCR fooling many to believe #CRISPR/#HDR events went smooth, use the remainder space on your poster 😂 read up here for fish https://t.co/8V3Gxprg35 & here for the mouse https://t.co/MkwJWlEmhS #GfE2024

[the Node @the_Node]

Are you a #DevBio researcher in Germany? Have you joined @GfEeV as a member yet?👇

[WittbrodtLab @WittbrodtLab]

First study showing 3D laser printing of #microimplants in living organisms is out, thanks to an awesome collaboration @EvaBlascoPo group. Check out the preprint on @biorxivpreprint  https://t.co/KEWb54GWiy
...
#2photonlithography #3dlaserprinting #medaka #drosophila

[WittbrodtLab @WittbrodtLab]

First study to implement morphogen gradients within #organoids is out! We collaborated with @KGoepfrich group, who made this possible with the use of DNA bead nanotechnology. Check out the preprint on @biorxivpreprint https://t.co/WefauK28JF
@AftingCassian @ToWalther

[bertalanffy. @BertalanffyCos]

Save the date! Wir freuen uns sehr den Rektor der Universität Heidelberg, Prof. Dr. Dr. h.c. Bernhard Eitel, am 10.07.2023 für die vierte Ausgabe unserer öffentlichen Podiumsdiskussion 'wissenschaft.leben' bei uns zu Gast zu haben😀

[Thomas Thumberger @_ThomThum_]

CRISPR-Fortschritte - Schnitt für Schnitt zu neuen Möglichkeiten: unser Review über sukzessive, kleine Fortschritte, die gezieltes Genomeditieren mittels CRISPR & BaseEditoren effizienter gemacht haben
https://t.co/xcj5Qn3uXh ✂️ mit @kaisa_pakari & @WittbrodtLab im @Spektrum_BIO

[bertalanffy. @BertalanffyCos]

Wer wollte nicht gern als Schüler mal einen Laborkittel überwerfen und erleben wie es ist an der Uni zu forschen ? Genau das ermöglicht unsere Bertalanffy Practical auf das sich Schüler der Oberstufe gerne bewerben können. Wir freuen uns gemeinsam mit euch zu forschen!

[Thomas Thumberger @_ThomThum_]

Just published @Dev_journal, Kaisa (@kaisa_pakari) presents our "inception" approach to target previously inaccessible nucleotides with base-editing at the 1st Heidelberg #CRISPR mini symposium @MKolleg today - link to paper: https://t.co/ggZkKV3uB5

[WittbrodtLab @WittbrodtLab]

Controls and Illumina seq show predictable & robust editing at the 2nd site only after new PAM creation. We demonstrate staggered non-synonymous codon changes and early STOP codons, removal of a splice acceptor and locally confined multi-codon editing to cause allelic variance.

[WittbrodtLab @WittbrodtLab]

Here it is: our #inception paper @Dev_journal „De novo PAM generation to reach initially inaccessible target sites for base editing“ by @kaisa_pakari & @_ThomThum_ We use 2 guides & 2 editors to edit distant nucleotides with NGG base-editors
https://t.co/E4Qm7dCpRu #medaka #DNA

[WittbrodtLab @WittbrodtLab]

This one-shot, two step approach uses sequential base editing. The 1st editor introduces a new PAM motif that is picked up by a 2nd editor to edit 27-36nts upstream of the original PAM. This method increases targeting flexibility across all genomes by 65% #genomeediting #CRISPR