Xiaochen Fan

PhD student in the Department of Eye and Vision Science at the University of Liverpool

[Xiaochen Fan @XiaochenFan1]

@BlandineP84 The TM tissue is a very thin tissue in the anterior chamber and the TM cells are relatively rare. When I dissected the TM tissue, I need to let them proliferate for 1 month to get enough numbers. Their proliferation speed will increase with the passages.

[Xiaochen Fan @XiaochenFan1]

Trabecular meshwork progenitor cell, a potential treatment for primary open-angle glaucoma. #HLSPosterDay2020
#HLSLCMS

[Xiaochen Fan @XiaochenFan1]

@BlandineP84 Yes, I think these cells are diverse. The TM cells we are working on is from the human donor eyes. We are interested in the behaviours of those progenitor cells in glaucoma conditions, so may try to collect some TM progenitor cells from glaucoma donors in the future studies

[Xiaochen Fan @XiaochenFan1]

@stephlharrison Hi Steph, thank you :). We normally dissect the TM tissue under the microscope. However, the TM tissue in aged and glaucoma donors is very thin and weak. I'm thinking about doing the cell staining before the dissection

[Xiaochen Fan @XiaochenFan1]

@Svgebn Thank you :)

[Xiaochen Fan @XiaochenFan1]

@BlandineP84 Thank you :) The flow cytometry (with sorting function) could be a good choice to help to purify the TM progenitor cells and my group is planning to run the single-cell sequence analysis to help to identify the multiple cell types in TM cultures.

[Xiaochen Fan @XiaochenFan1]

@simon_too I think the culture could affect the phenotype include the serum effect and also the culture plate. I'm planning the find serum-replacement culture medium and trying to use the suspension plate to culture my progenitor cells🙂

[Xiaochen Fan @XiaochenFan1]

@simon_too Hi, thank you. it's a really good question. My group has recently done the RNA sequence analysis about the TM cells and their progenitor cells to see the gene expression and planned to do the single-cell analysis to identify the different cell type in the TM culture.