and inducing S phase arrest in CRC cells, suggesting that ivermectin might be a new potential anticancer drug therapy for human colorectal cancer and other cancers.
Following NAC treatment, the inhibition of cell growth induced by ivermectin was reversed. Finally, ivermectin at low doses (2.5 and 5 µM) induced CRC cell arrest. Overall, ivermectin suppressed cell proliferation by promoting ROS-mediated mitochondrial apoptosis pathway
and cleaved PARP and downregulated antiapoptotic protein Bcl-2. Mechanism analysis showed that ivermectin promoted both total and mitochondrial ROS production in a dose-dependent manner, which could be eliminated by administering N-acetyl-l-cysteine (NAC) in CRC cells.
cycle. The results demonstrated that ivermectin dose-dependently inhibited colorectal cancer SW480 and SW1116 cell growth, followed by promoting cell apoptosis and increasing Caspase-3/7 activity. Besides, ivermectin upregulated the expression of proapoptotic proteins Bax
apoptosis, used Caspase 3/7 Activity Apoptosis Assay Kit to evaluate Caspase 3/7 activity, used Western blot to determine apoptosis-associated protein expression, and used flow cytometry and fluorescence microscope to determine the reactive oxygen species (ROS) levels and cell
The objective of this study was to evaluate the influence of ivermectin on CRC using CRC cell lines SW480 and SW1116. We used CCK-8 assay to determine the cell viability, used an optical microscope to measure cell morphology, used Annexin V-FITC/7-AAD kit to determine cell
Colorectal cancer (CRC) is the third most common cancer worldwide and still lacks effective therapy. Ivermectin, an antiparasitic drug, has been shown to possess anti-inflammation, anti-virus, and antitumor properties. However, whether ivermectin affects CRC is still unclear.
#Binance sent $100's of million in #cryptocurrency to Wintermute to force capitulation.
#Wintermute is a company that works as a #crypto market maker and liquidity provider. In simple terms, it helps make buying and selling cryptocurrencies easier by always being ready to trade large amounts.
This means that when people want to buy or sell coins, Wintermute is there to keep the market active and prices moving smoothly.
Market makers like #Wintermute manipulate prices through circular trading. #Binance sells to Wintermute, who sells to #Coinbase, then back to Binance who are all market makers. This inflates trade volume without real demand, pushing prices down.
By dumping assets, they trigger fear, stop-losses, and liquidations, fueling shorts and driving prices lower. Retail traders panic sell, while market makers profit from shorts and buy back cheap. It’s a controlled cycle to manipulate sentiment and price. Beware of these tactics.
How This Manipulates Prices Down and Fuels Shorts
1. High Trade Volume With Minimal Demand:
The repeated cycling of assets between these entities artificially inflates trading volume, making the market appear highly active.
Despite high activity, there's no new buyer demand entering the system, which prevents price recovery.
2. Dumping of Assets:
Wintermute or similar market makers can sell large volumes of assets across multiple exchanges (e.g., Binance and Coinbase). These bulk sales push prices downward due to the basic supply-demand imbalance (oversupply with weak demand).
Market makers are incentivized to lower prices intentionally to accumulate cheaper assets or profit from other strategies like short selling.
3. Short Selling Profits:
By driving the price lower, market makers fuel negative sentiment. Traders see falling prices and assume a bearish trend is forming, leading to increased short positions.
Wintermute, Binance, or Coinbase can capitalize on this by shorting the market, profiting from the price decline they orchestrated.
4. Reinforcing Downward Spirals:
As prices drop, stop-loss orders and liquidation of leveraged positions trigger further sales, accelerating the downward trend.
This cascading effect creates a bearish cycle, driving prices lower and further enriching market makers and shorts.
5. Market Sentiment Manipulation:
This cycle affects investor sentiment. Declining prices and high volume create fear among retail traders, leading to panic selling. This enables large players to scoop up assets at bargain prices once their shorting strategy concludes.
#draintheexchange #crypto #Altseason