AJ Sethi

RNA: splicing, modification + transcription (he/him)

[AJ Sethi @_AJSethi]

@jrkelly @saltynostrils 💯

[AJ Sethi @_AJSethi]

@arjunrajlab scaling up throughput doesn't increase signal to noise ratio. there is a huge need to improve signal across most targeted assays.

[AJ Sethi @_AJSethi]

@NikoMcCarty super cool! i tried to give it a nucleus:
https://t.co/yb8cgElVM0

[AJ Sethi @_AJSethi]

@silvirouskin

[AJ Sethi @_AJSethi]

@sonohoor PhD years 3-5

[Eduardo Eyras @EduEyras]

SWARM (Single-molecule Workflow for Analysing RNA Modifications) detects pseudouridine, m6A, and m5C on individual molecules from direct RNA nanopore signals (in both chemistries RNA002 and RNA004) https://t.co/iMg2TWDKAO see more on poster P2-396 at #rna2025 #RNA25 #RNAmods

[AJ Sethi @_AJSethi]

Thanks to all co-authors, without whom this work would be impossible. @GuarnacciMarco
@starstuffkarthi
@pol2rna
@TominAustralia
@RippeiH
and others.

[AJ Sethi @_AJSethi]

We describe the temporal organisation of central pre-mRNA processing events, including splicing, m6A modification and 3' end formation, during mRNA synthesis. Check out the preprint here!

[Eduardo Eyras @EduEyras]

"Single-molecule multimodal timing of in vivo mRNA synthesis" a collaboration with Rippei Hayashi's lab, work led by AJ Sethi @_AJSethi #RNA #premRNA #splicing #RNAprocessing #m6A #polyadenylation https://t.co/3sQf13qfoC

[AJ Sethi @_AJSethi]

dFORCE is directly applicable to mammalian tissue samples, and produces results consistent with cell culture, unlocking analysis of mRNA synthesis kinetics in vivo:

[AJ Sethi @_AJSethi]

@ItaiYanai

[AJ Sethi @_AJSethi]

it gets me fr

[Linus ✦ Ekenstam @LinusEkenstam]

Ask ChatGPT

“based on what you know about me. draw a picture of what you think my current life looks like”

past your responses below.

thanks again @mreflow & @danshipper

[Eduardo Eyras @EduEyras]

R2Dtool https://t.co/czxyDjWT94 for handling, integrating, and viualising isoform-resolved RNA feature data - led by @_AJSethi #bioinformatics #RNA #isoforms
https://t.co/wPGGZxHarv

[AJ Sethi @_AJSethi]

@ribonucleicacid The spliced AS transcripts could also be RT artefacts. I don't see almost any antisense mRNA reads in cerevisiae nanopore RNA libraries.

[AJ Sethi @_AJSethi]

@ribonucleicacid And a little further down at RPS18B:

[AJ Sethi @_AJSethi]

@ribonucleicacid I'm seeing splicing of sense introns on the antisense transcripts, which isn't possible if these transcripts arise from genomic transcription. Could the AS transcripts come from RNA-dependant RNA polymerase transcription of processed mRNA?

RPS17A in SRR27483326 shown:

[Cell @CellCellPress]

Now online! Capturing totipotency in human cells through spliceosomal repression https://t.co/HKQq9ntqQi

[Eduardo Eyras @EduEyras]

It's finally published. This one took a long time to get through reviewers: Prediction of m6A and m5C at single-molecule resolution reveals a transcriptome-wide co-occurrence of RNA modifications https://t.co/pE7u1lQo3V