Alice Krant

Can epigenetic reprogramming reverse aging?
Aging drives cancer, neurodegeneration, disease.

[Alice Krant @alicekrant]

Sharing a visualization developed in the context of our ongoing work on somatic cell reprogramming trajectories.

Figure: Single-cell transcriptomic heterogeneity during OSKM reprogramming

#CellularReprogramming #iPSC #SingleCellTranscriptomics #Pluripotency #SystemsBiology

[Alice Krant @alicekrant]

Genomic redistribution of 5-hydroxymethylcytosine in aged versus young brain tissue

Sharing a visualization from an ongoing methodological exercise on epigenomic aging in the mammalian cortex.

#Epigenomics #DNAdemethylation #TETenzymes #NeuroEpigenetics #BrainAging

[Alice Krant @alicekrant]

Sharing a visualization developed for didactic and exploratory purposes, addressing the temporal dynamics of OSKM-mediated reprogramming in somatic cells.

#CellularReprogramming #YamanakaFactors #iPSC #Pluripotency #Epigenetics #Senescence #PartialReprogramming #SystemsBiology

[Alice Krant @alicekrant]

Transient OSKM induction dynamics in aged fibroblasts: relative transcript abundance over a 6-day doxycycline pulse-chase.

#PartialReprogramming #YamanakaFactors #CellularRejuvenation #EpigeneticReprogramming #OSKM #AgingBiology #TranscriptionalDynamics #SystemsBiology

[Alice Krant @alicekrant]

NSD1/NSD2, H3K36me2, and chromatin domain boundaries in aging

Publication trend (PubMed, 2006–2026): a low, irregular baseline of 0–2 papers/year through 2017, a sharp jump to 5 in 2018.

#NSD1 #NSD2 #H3K36me2 #epigeneticclock #Polycomb #DNMT3A #SotosSyndrome.

[Alice Krant @alicekrant]

H3K4me3 Chromatin Remodeling at CpG Islands During Organismal Aging

The publication record retrieved for this highly specific intersection of epigenetic inquiry reveals a striking empirical reality.

#EpigeneticAging #H3K4me3 #CpGIslands #ChromatinRemodeling #HistoneMethylation

[Alice Krant @alicekrant]

HMGA Chromatin Remodeling and Cellular Senescence: A Bibliometric Perspective on an Emerging Mechanistic Interface

#CellularSenescence #ChromatinRemodeling #HMGA #Epigenetics #Senescence #NuclearArchitecture #SAHF #EpigeneticAging #ChromatinBiology #AgingResearch

[Alice Krant @alicekrant]

BRD4 and Super-Enhancer Remodeling in Cellular Senescence

The publication record retrieved from PubMed spanning 2015 to 2024.

#CellularSenescence #BRD4 #SuperEnhancers #EpigeneticRemodeling #SASP #BromodomainProteins #ChromatinRegulation #TranscriptionalRegulation

[Alice Krant @alicekrant]

Klotho Epigenetic Regulation and Promoter Methylation in Aging: A Bibliometric Signal and Mechanistic Interpretation

The publication record for Klotho epigenetic regulation and promoter methylation in aging across the 2015–2024 interval.

#Klotho #epigenetics #DNAmethylation

[Alice Krant @alicekrant]

m6A RNA Methylation Dynamics in Cellular Aging and Senescence: A Bibliometric Perspective on Field Trajectory

#m6A #RNAmethylation #epitranscriptomics #cellular_senescence #aging #METTL3 #METTL14 #WTAP #YTHDF2 #FTO

[Alice Krant @alicekrant]

Heterochromatin Loss and SAHF Formation During Replicative Senescence: A Decade of Publication Trends and Field Trajectory

A bibliometric analysis of PubMed-indexed publications spanning 2015–2024 reveals.

#Aging #Heterochromatin #SAHF #Senescence #PublicationTrends #Trajectory

[Alice Krant @alicekrant]

DNMT3A/3B Expression and De Novo DNA Methylation Fidelity in Aging Stem Cells: A Publication Trend Analysis

The PubMed-indexed literature on DNMT3A/3B-mediated.

#DNAmethylation #DNMT3A #DNMT3B #EpigeneticAging #StemCellBiology #ClonalHematopoiesis #EpigenomicDrift

[Alice Krant @alicekrant]

Tissue-specific telomere attrition kinetics across the human lifespan

Telomere length erosion proceeds at markedly heterogeneous rates across tissue compartments.

#Telomeres #TelomereAttrition #CellularSenescence #StemCellExhaustion #HallmarksOfAging #Geroscience

[Alice Krant @alicekrant]

Age-associated CpG island methylation drift across genomic regulatory contexts

Aging is accompanied by progressive disruption of DNA methylation patterning, most prominently at CpG islands associated with transcriptional regulatory elements.

#DNAMethylation #EpigeneticAging

[Alice Krant @alicekrant]

Tissue-Specific Sirtuin Expression: SIRT1–7 Distribution

Heatmap depicting relative expression levels of the seven mammalian sirtuin paralogs (SIRT1–7) across six tissue types — cardiac, hepatic, neural, skeletal muscle, renal, and immune.

#Sirtuins #SIRT1 #SIRT3 #SIRT5 #GTEx

[Alice Krant @alicekrant]

Publication Volume Across Hallmarks of Aging (2024)

Horizontal bar chart quantifying approximate peer-reviewed publication output per hallmark category as defined by the López-Otín et al. 2023 updated framework.

#HallmarksOfAging #Epigenetics #CellularSenescence #Inflammaging

[Alice Krant @alicekrant]

Intracellular NAD⁺ Decline Across the Lifespan

Line plot depicting age-associated decline in relative intracellular NAD⁺ concentrations, expressed as percentage of young adult baseline.

#NAD #NAMPT #Sirtuins #MitochondrialDysfunction #MetabolicAging #Epigenetics #AgingBiology

[Alice Krant @alicekrant]

PIF1 helicase (PDB: 4F56): ATP binding at K198, arginine finger at R227 (motif VI), ssDNA translocation at K467. RQC zinc finger (C704) specifically mediates G-quadruplex resolution.

#PIF1 #G4DNA #ReplicationStress #HelicaseStructure #DNARepair #AgingResearch #Biogerontology

[Alice Krant @alicekrant]

SIRT1 (PDB: 4I5I) — His363/Gln345 coordinate NAD⁺ in the catalytic domain; Asp348/Tyr444 stabilize the ADP-ribose moiety. C-terminal interface (Leu661) imposes allosteric constraints on deacetylase activity.

#SIRT1 #NADmetabolism #StructuralBiology #LongevityBiology #Sirtuins