XtraBio

✍️ looking for a lab internship opportunity 🤝

[XtraBio @bio_xtra]

@SebasP49474734 @Tokenicer @Grokh Why is this good? I mean if they do this way the price aren’t effected - why is it good?

[XtraBio @bio_xtra]

@Raeubertochtah Welche Flagge ist das nochmal? Habs jahrzehnte nicht mehr gesehen

[XtraBio @bio_xtra]

@ISOjish Accurate

[XtraBio @bio_xtra]

@Whhyzz @WatcherGuru btc*

[XtraBio @bio_xtra]

@Whhyzz @WatcherGuru No, the Max Pain Level for byc is at $114.000
So we’ll get a strong sell, to get as close as possible to the MPL

[XtraBio @bio_xtra]

@BitbullTrading Lasset die fomo beginnen

[XtraBio @bio_xtra]

@papigalang504 @KimaNetwork 😂😂

[XtraBio @bio_xtra]

@verasitytech @soonchain_ai Just another random partner. Lol

[XtraBio @bio_xtra]

@elonmusk As long as it‘s not an aluminum hat💁🏼‍♂️😂

[XtraBio @bio_xtra]

5️⃣ You confirmed your molecule — now test it in biology.

Time for in vitro testing to see how it behaves in cells.
Does it kill them? Protect them? Interact with a target?

Here’s what to do:

📌 Step 1: Choose The Right Cell Line

→ You’ll need cells that are relevant to your molecule’s goal. These are called immortalized cell lines, and you can buy them from trusted suppliers:
ATCC 🔹 https://t.co/7u1vmlxIdt
DSMZ 🔹 https://t.co/ZmvWWBIDE6

→ Relevant to your target:
Cancer 🔹 HeLa(Cervical), MCF-7(breast), A549(lung)
Liver function / Toxicity 🔹 HepG2
Brain / Neurons 🔹 SH-SY5Y
Immune System 🔹 Jurkat (T Cells)

📌 Step 2: Expose Cells To Different Concentrations

→ Create a dose–response curve
→ Prepare serial dilutions (0.1 µM, 1 µM, 10 µM, 100 µM…)
→ Seed cells into a 96-well plate
→ Add your molecule to the wells in different concetration
→ Incubate the plate usually 24-72 hours in CO₂ incubator
Alternatives for CO₂ incubator(because it‘s expensive):
DIY-CO₂-Incubator ~200–500 $ or
Choose cell lines that don‘t require CO₂ and
Keep cells at 37°C in a basic incubator or heated box

⚠️ Note: Not all cell lines grow equally well in these media ⚠️ Check the datasheet or literature
⚠️ ATCC - https://t.co/hZbmxmyhXj Each cell line page includes a „Culture Conditions“ section.
⚠️ Example: SW480 (at ATCC)
→ Grown at 37°C without CO₂, using Leibovitz’s L-15 medium.

📌 Step 3: Run Standard Assays:

• Viability: MTT, resazurin
→ how many cells are still alive
• Membrane damage: LDH release
→ dead cells leak LDH - measures cell damage
• Apoptosis/necrosis: Annexin V / PI staining
→ shows programmed cell death
• Oxidative stress: ROS assays
→ measures reactive oxygen species
• Reporter genes: pathway activation
→ shows if a cellular pathway is triggered

📌 Step 4: Analyze The Data

→ Calculate IC₅₀ - Identify toxic vs. safe ranges
→ See how your molecule acts in a living system (well, living cells)
→ Dose-response curve: Effect vs. Concentration (Michaelis-Menten Analysis)

⚠️ Doesn‘t worked well?
⚠️ Repeat🤣

#InVitro #CellBiology #Toxicology #MTT #DrugDiscovery #LabWork #MolecularTesting

[XtraBio @bio_xtra]

Most people stop at drawing molecules.
But you?
You want to build one — and test it.

This thread is your complete guide to turning a structure into a real, tested compound.
From SMILES to synthesis. From cells to answers.

Let’s go.
#DrugDiscovery #ScienceTwitter #InVitro

[XtraBio @bio_xtra]

4️⃣ You made your molecule — now prove it.
How do you know it’s really what you designed?

Here’s how to confirm your compound’s identity with chemistry’s most powerful tools:

📌 NMR – Nuclear Magnetic Resonance ($50-120)
→ Reveals how H and C atoms are connected
→ Confirms structure & functional groups

📌 MS – Mass Spectrometry ($50-150)
→ Exact molecular weight
→ Sees fragments of your molecule

📌 IR – Infrared Spectroscopy ($20-60)
→ Confirms OH, COOH, C=O, and more

Where do you do this?
🏛️ University lab
🧪 Companies (Eurofin, Sigma-Aldrich, Intertek)

You can send your sample to certified analytical chemistry lab, for example:
Eurofins 🔹 https://t.co/tcQ0LL9AgN
AlsaChem 🔹 https://t.co/bAV8I7b8zE
Intertek 🔹 https://t.co/nqlYIf2GlT
Sigma-Aldrich Analytical Services 🔹 https://t.co/yuco21KK9V

Result?
Spectra + interpretation = structural proof
Now you’re ready for bio testing!

⚠️ → Part 5: Let‘s test in biology!

#AnalyticalChemistry #NMR #MassSpec #Infrared #DrugDevelopment #MoleculeMadeReal

[XtraBio @bio_xtra]

5.) Many different signalling pathways can cause - or fail to suppress - cell proliferation.
But what we learned from the case of mutant EGFR is that it might be possible to treat certain cancers if we can disrupt the right signalling pathways.

[XtraBio @bio_xtra]

A generic #cellular #signalling #pathway with kinase.

Kinases phosphorylate - that‘s what they do.
Phosphorylation performs a function a bit like turning a switch on or off. In this way, a cascade of signals is created.
One such process is cell division.
One phosphorylated molecule often phosphorylates hundrets more and each of them hundrets more, and so on.

[XtraBio @bio_xtra]

4.) This is what happens when the EGFR gene is muted in lung cancer: the tyrosine kinase switch it encodes becomes permanently locked „on“.
The signalling pathway instructs cells to keep deviding, producing new cells faster than they are eliminated.