DerekIsWho

[DerekIsWho @biochem21z]

@wingoffire 小孩小学的班主任上学期差不多一个月就会发一次邮件，说班上有学生被跳蚤咬了，让家长回家检查自己小孩。

[DerekIsWho @biochem21z]

@lanque300825 我当年就有同宿舍的大学同学通过SM3去了新加坡，后来博士毕业进了新加坡美光，linkined一看在那也呆了快十年了，股票应该都赚翻了

[DerekIsWho @biochem21z]

@Barchart The unit price of many grocery products are more expensive than Trader Joe's, but come in bulks. Just do not make sense at all

[DerekIsWho @biochem21z]

@yuyy614893671 输？舒？

[DerekIsWho @biochem21z]

@stevehou @manyapan 你说咧 (你共咧），还不错？

[DerekIsWho @biochem21z]

@saonanaa 去年暑假还真就这样买过一次儿童票，还是列车乘务员教的。带小孩坐高铁去香港，不到10岁，大陆段是不需要票的，但是到香港出站需要儿童票，没票还要罚全程的款。乘务员让买了当天后面的车次，再把大人票退了。

[DerekIsWho @biochem21z]

@DongLi64670501 @zcwzhy @grok can you find the review paper this guy is talking about on Journal of Translational Medicine?

[DerekIsWho @biochem21z]

@biantaishayu 说明这个基因很重要啊，敲除的就赶紧把过表达老鼠����搞起来，过表达的赶紧搞敲除的

[DerekIsWho @biochem21z]

@xiaohu 一个月的房租可以买4个，但是MacBook不一定要买，房租一定得交啊

[DerekIsWho @biochem21z]

@wangwatchworld 啧啧啧，在峰尖里头

[DerekIsWho @biochem21z]

@manateelazycat NouTube, SimpMusic

[Niko McCarty. @NikoMcCarty]

Rubisco is (arguably) the most abundant protein on Earth. (LPP surely comes close, right?) It’s an enzyme that fixes CO₂ into sugars during photosynthesis.

Unfortunately, as most people learn in school, Rubisco is inefficient. Sometimes it confuses O₂ for CO₂ and wastes energy. Plants make up for this in raw concentration; up to half the soluble protein in a leaf is Rubisco.

People have been trying to engineer better Rubiscos for many decades, but it's not easy because the proteins are big, do not fold easily (they need chaperone proteins to help out), are made from 16 subunits in land plants.

But there's a new paper in Nature Plants that looks really interesting. The TL;DR is that a group in Australia figured out how to express plant Rubiscos (and all SEVEN of their folding chaperones) using a set of 3 plasmids inside of E. coli cells. This enabled them to do "directed evolution" of Rubisco in bacterial cells, and quickly find Rubisco mutants that have higher enzymatic efficiency or that fold better.

In addition to the 3 plasmids, the researchers also coaxed E. coli to make ribulose-1,5-biphosphate, or RuBP, which is the 5-carbon sugar that Rubisco smashes into carbon dioxide to make molecules of 3-PGA for central metabolism.

Now, the clever bit is that you RANDOMLY MUTATE the three plasmids encoding the Rubisco to make millions of variants. Then, you transform those mutated plasmids into E. coli. If the E. coli do NOT make a functional Rubisco, RuBP levels build up and kill the cell; the molecule becomes toxic. But if the E. coli DO make a functional Rubisco, then they keep the RuBP levels in check and live just fine.

Using this "screening assay," the researchers found 46 fast-growing colonies of E. coli. Two of those colonies encoded really useful mutations. One mutation (M116L) makes Rubisco about 25–40% faster. The other (A242V) makes it fold and assemble much more efficiently.

They put this mutation into a "hybrid Arabidopsis–tobacco Rubisco," put that into tobacco plants, and measured growth. The plants with M116L grew 75% faster than wildtype.

No guarantees this will scale to more useful crops, like wheat and corn and soybeans etc. But it seems like a nice in vitro assay for faster prototyping!

[DerekIsWho @biochem21z]

@hsn8086 最爱必应词典app，是的，已经被砍掉了

[DerekIsWho @biochem21z]

@wangwatchworld 那都是对外宣传，对外忽悠，自己是不可能信的

[DerekIsWho @biochem21z]

@FanrongY 这不就是党大还是法大的具象化

[DerekIsWho @biochem21z]

@hsn8086 电焊一下，又不是不能用

[DerekIsWho @biochem21z]

@Richard77778888 @RickyYu6612 Total sugar 26g

[DerekIsWho @biochem21z]

@Richard77778888 三番附近早晚还是蛮冷的

[DerekIsWho @biochem21z]

@jefflijun 所以生育率下降是最后的反抗

[DerekIsWho @biochem21z]

@biantaishark https://t.co/we29EDdI65

[Douglas Yao @DouglasYaoDY]

Biology’s sub-25% replication rate is an EXISTENTIAL issue for the field.

Most academics don’t seem to care, which shows that their priorities are not in doing good science.