Darren Cusanovich

Finnish-American Genome Scientist. Assistant Professor at @UArizonaCMM. I think about single-cell genomics, development, complex diseases of the airway.

[Darren Cusanovich @cusanovich]

So excited to share that my @NIGMS ESI MIRA (R35) was officially funded Friday! The grant (5 years) will support our efforts to develop better single-cell genomic technologies. If you're interested in working on single-cell tech in an affordable town, consider @UArizonaCMM @UofA!

[Darren Cusanovich @cusanovich]

This was a great collaboration between the @cusanovich and @acadey80 labs, driven by @haozhangutsa and Ryan Mulqueen. But it also depended on important contributions from @galliganlab, @Dr_Ledford, and @FrancyPolverino, among others. Thank you to all for your efforts! (13/13)

[Darren Cusanovich @cusanovich]

Hey again, science people! To reiterate, the Cusanovich lab released 2 preprints last week! Worth repeating! Here's a 🧵on txci-ATAC-seq (https://t.co/xBgHsBhOn8), a method for multiplex single-cell ATAC-seq marrying combinatorial indexing and 10x microfluidic barcoding. (1/13)

[Darren Cusanovich @cusanovich]

This work highlights the value of ATAC-seq for checking KO mouse line genotypes of a certain vintage, and the complex regulatory effects of genetic variation across cell types. We hope txci-ATAC enables large chromatin studies and better study design in sc genomics. (12/13)

[Darren Cusanovich @cusanovich]

In addition to the driving force of the unstoppable HyunJin Kang, this work involved important contributions from John Fitch in the Flow Cytometry Shared Resource at UA and Reeba Varghese and @CurtisThorne. Thanks all! (7/7)

[Darren Cusanovich @cusanovich]

Hey science people! Happy to report the Cusanovich lab released 2 preprints last week! First, a 🧵 on our preprint (https://t.co/T4U5LQNnKj) describing a CRISPR-based transcriptional cascade system using Cas12a – we call the system SGRN (and pronounce it like “sojourn”). (1/7)

[Darren Cusanovich @cusanovich]

Importantly, the more complex the cascade (e.g. 3 levels instead of 2) the more pronounced the increased activity. There are many directions for future work, but we hope this system can help future synthetic biology efforts and validation experiments of single-cell results. (6/7)