Quigly Dragotakes

PhD in Molecular Microbiology and Immunology from Johns Hopkins BSPH
Postdoctoral researcher in the Casadevall Lab,
Yeast enthusiast

[Quigly Dragotakes @dragotakes]

Recently upgraded my Cryptococcus capsule measuring script and repackaged it as a single install file that does NOT require matlab. Still need to manually check the result images to ensure accuracy but between me and my undergrad we made 94k measurements with minimal effort.

[Quigly Dragotakes @dragotakes]

Long wait but worth it for this coffee raccoon #ASMicrobe

[Quigly Dragotakes @dragotakes]

@Danfqsmith I think its an anti-theft dye pack for clothes and it's obviously there to stop people from stealing the tree

[Quigly Dragotakes @dragotakes]

@caipcoelho @fungalhyphae @MRCcmm @DrMukaremera @BallouLab Alright, I think it's good to go at least for testing. Let me know if you run into issues, or features you wish where included. You can download the installer from this repo:
https://t.co/REVvtDpzy8

[Quigly Dragotakes @dragotakes]

Recently upgraded my Cryptococcus capsule measuring script and repackaged it as a single install file that does NOT require matlab. Still need to manually check the result images to ensure accuracy but between me and my undergrad we made 94k measurements with minimal effort.

[Quigly Dragotakes @dragotakes]

@fungalhyphae @MRCcmm @caipcoelho @DrMukaremera @BallouLab Yeah of course, and I could always use feedback / suggestions. There are a couple things I need to finish before uploading, maybe a day or two. Carolina is probably familiar with the old version and this one is very similar to use, should be straightforward.

[Quigly Dragotakes @dragotakes]

@fungalhyphae Both! It spits out both a copy of the image with the circles drawn on it so you can make sure they look right and an excel sheet with the compiled information of each detected cell. If it only detects a capsule the right half of the columns will be empty so easy to filter out

[Quigly Dragotakes @dragotakes]

Who you calling Pinhead?

[Quigly Dragotakes @dragotakes]

Updated the bot a little bit so now the image preview includes the whole paper since it wasn't clear to start with that the bot makes a full paper, not just an abstract. Will next try adding figure AI and better cohesion between sections.

[Quaglee Dragontacos @quaglee]

Check out my newest paper (Full text available in bio link): Could Histoplasma capsulatum Be Replicated and Evolved into Paracoccidioides Genus Geographical Distance Ahead?

[Arturo Casadevall @ACasadevall1]

The main message in this paper is that Cryptococcus neoformans releases proteins inside macrophages that modify host cell function. Getting this story out took almost a decade and was the work of many. @caipcoelho @dragotakes @Danfqsmith @marclrodrigues

[Quigly Dragotakes @dragotakes]

@MarioPrime Easy pick, no way I'm voting for Mark Zucusberg

[Quigly Dragotakes @dragotakes]

Looks like science is still safe though

[Quigly Dragotakes @dragotakes]

Finally approved for the DALLE art AI, got some things I want to try but of course I had to start with a rigorous test of its ability. Thus, I present:

"An oil painting of a minion marrying garfield"

I regret it immediately, but am impressed an AI could generate this on its own

[Quigly Dragotakes @dragotakes]

@JorgeAmich Thanks for the interest! Mechanism is definitely my next focus, there must be some really cool interactions between yeast and host to coordinate the whole process.

And absolutely, I think naming it after myself was one of my best jokes.

[Quigly Dragotakes @dragotakes]

Not sure why one replicate of condition 1 is so weird, maybe flocculation, but my benchtop growth curve machine finally fully works!

[Quigly Dragotakes @dragotakes]

Mk I of new pet project to make small/cheap growth curve reader. Test curve looks good, next step to test precision/replicability. Total cost < $200 though I think that can be reduced and size of p1000 tip box. Just stick it on an shaker in an incubator when you need to use it.

[Quigly Dragotakes @dragotakes]

Figured out the power issue but I may have switched a couple sensor wires when replacing a board so I'm not sure if the well designations are accurate and can't compare conditions yet (will fix monday). Regardless, all 12 sensors appear to work fine over a 50 hour growth curve.

[Quigly Dragotakes @dragotakes]

@danquinnsmith Do you think the faded one is just an older less accurate map from the days the drink is harkening back to? Everything looks a little wonky in it.