Małgorzata Figiel

[Małgorzata Figiel @drfigiel]

I wish to thank all the co-authors from @IIMCB_Poland and from @hauryliuk lab at @lunduniversity ! @SOLARIS_science

[Małgorzata Figiel @drfigiel]

Excited to share our preprint exploring the structural, biochemical and in vivo properties of DRT7/UG10 reverse transcriptases which produce dsDNA using a combination of primase and polymerase activities and provide broad-spectrum antiphage defense!
https://t.co/2pnX6UnI5c

[Małgorzata Figiel @drfigiel]

E. coli DRT7 provides defense against a very broad range of phages and uses a direct defense mechanism. The escape mutants of phage lambda and Bas1 that overcame this protection carried mutations in genes encoding a DNA mimic and a putative phosphoesterase, respectively.

[Małgorzata Figiel @drfigiel]

@MariuszCzC @11_Shuv_52 I wish to thank all the coauthors of this study and our colleagues from Nowotny lab. We are also grateful to @IIMCB_Poland, @NCN_PL and @SOLARIS_science for making this project possible!

[Małgorzata Figiel @drfigiel]

I am very excited to share our recent preprint in which we are structurally investigating macromolecular complexes that contain DNA-binding proteins ICP8 of Herpes Simplex Virus 1 and BALF2 of the Epstein-Barr Virus.
https://t.co/p5nKk6BLeM

[Małgorzata Figiel @drfigiel]

@MariuszCzC @11_Shuv_52 Finally, we deliver the first complete cryo-EM structure of a two-component recombinase composed of ICP8 annealase and UL12 alkaline nuclease. The complex was reconstituted using a model DNA with a dsDNA and 3′ ssDNA overhang.

[Małgorzata Figiel @drfigiel]

@MariuszCzC @11_Shuv_52 A very similar DNA conformation is observed in the crystal structures of ICP8 with 14-mer ssDNA. ICP8 subunits form dimers with a two-fold symmetry, with their DNA-binding sites facing each other and the outward-rotated bases of bound DNAs form pairs in a flexible manner.

[Małgorzata Figiel @drfigiel]

@MariuszCzC @11_Shuv_52 We determined a cryo-EM structure of BALF2 bound to ssDNA at 3.2 Å resolution. The visible fragment of DNA is accommodated in a positively charged cleft. In the first four nucleotides, the base edges face the protein, but the next three bases are rotated away from the protein.

[Małgorzata Figiel @drfigiel]

We are very happy to share the preprint of our latest study on the DNA-binding proteins from Herpes Simplex Virus-1 and Epstein-Barr Virus! @IIMCB_Poland

[bioRxiv Molecular Biology @biorxiv_molbio]

Structural insights into DNA annealing and recombination by herpesviral DNA-binding proteins ICP8 and BALF2 https://t.co/6apvdVCLj1 #biorxiv_molbio

[Małgorzata Figiel @drfigiel]

Following up on our paper about AbiK and Abi-P2, we now present an in-depth characterization of the third Abi polymerase, AbiA, performed in collaboration with @hauryliuk lab and @DziembowskiLab.
https://t.co/kuzz44OCM3

[Małgorzata Figiel @drfigiel]

Great job, congratulations to Shivlee and Mariusz!

[Shivlee Nirwal @ShivleeNirwal]

Very glad to share that our work on the RecFOR pathway is finally published in @NatureSMB! @MarcinNowotny @IIMCB_Poland @MariuszCzC. Many thanks @SOLARIS_science @NCN_PL #cryoEM. (1/2)
https://t.co/VFSZgLqiqM

[Małgorzata Figiel @drfigiel]

@NaveedJ01722071 @IIMCB_Poland @MarcinNowotny @gmichlew Great! You can apply via this website: https://t.co/2lmkwYZQl8
Looking forward to hearing from you!

[Małgorzata Figiel @drfigiel]

@JacekJaworski10 @IIMCB_Poland @NCN_PL Thanks, Jacek! ☺️ I am super stoked about this project already!

[Małgorzata Figiel @drfigiel]

@its_rkgupta @IIMCB_Poland @NCN_PL Thank you, Rishi!

[IIMCB, Warsaw, Poland @IIMCB_Poland]

➡We are starting recruitment for #Warsaw4PhD within @IIMCB_Poland
➡We offer the opportunity to join 2️⃣ unique projects under the guidance of outstanding experts:
prof.@MarcinNowotny dr #MalgorzataFigiel & prof. @gmichlew
👉Recruitment details: https://t.co/uSlIdxz6Ax