Excited to share our preprint exploring the structural, biochemical and in vivo properties of DRT7/UG10 reverse transcriptases which produce dsDNA using a combination of primase and polymerase activities and provide broad-spectrum antiphage defense!
https://t.co/2pnX6UnI5c
E. coli DRT7 provides defense against a very broad range of phages and uses a direct defense mechanism. The escape mutants of phage lambda and Bas1 that overcame this protection carried mutations in genes encoding a DNA mimic and a putative phosphoesterase, respectively.
I am very excited to share our recent preprint in which we are structurally investigating macromolecular complexes that contain DNA-binding proteins ICP8 of Herpes Simplex Virus 1 and BALF2 of the Epstein-Barr Virus.
https://t.co/p5nKk6BLeM
@MariuszCzC@11_Shuv_52 Finally, we deliver the first complete cryo-EM structure of a two-component recombinase composed of ICP8 annealase and UL12 alkaline nuclease. The complex was reconstituted using a model DNA with a dsDNA and 3′ ssDNA overhang.
@MariuszCzC@11_Shuv_52 A very similar DNA conformation is observed in the crystal structures of ICP8 with 14-mer ssDNA. ICP8 subunits form dimers with a two-fold symmetry, with their DNA-binding sites facing each other and the outward-rotated bases of bound DNAs form pairs in a flexible manner.
@MariuszCzC@11_Shuv_52 We determined a cryo-EM structure of BALF2 bound to ssDNA at 3.2 Å resolution. The visible fragment of DNA is accommodated in a positively charged cleft. In the first four nucleotides, the base edges face the protein, but the next three bases are rotated away from the protein.
We are very happy to share the preprint of our latest study on the DNA-binding proteins from Herpes Simplex Virus-1 and Epstein-Barr Virus! @IIMCB_Poland
Following up on our paper about AbiK and Abi-P2, we now present an in-depth characterization of the third Abi polymerase, AbiA, performed in collaboration with @hauryliuk lab and @DziembowskiLab.
https://t.co/kuzz44OCM3
@NaveedJ01722071@IIMCB_Poland @MarcinNowotny @gmichlew Great! You can apply via this website: https://t.co/2lmkwYZQl8
Looking forward to hearing from you!
➡We are starting recruitment for #Warsaw4PhD within @IIMCB_Poland
➡We offer the opportunity to join 2️⃣ unique projects under the guidance of outstanding experts:
prof.@MarcinNowotny dr #MalgorzataFigiel & prof. @gmichlew
👉Recruitment details: https://t.co/uSlIdxz6Ax