Elizabeth Vaisbourd

PhD student fascinated by all things E. coli 🧫🦠 @WeizmannScience

[Elizabeth Vaisbourd @elizabethvaisb]

@yonchem Well-deserved Yonatan!

[Eric Topol @EricTopol]

The harmful and biased medical advice from social medial influencers, highlighting lack of expertise, and financial conflicts. Many specific examples and recommendations provided @bmj_latest
https://t.co/zFjaqQvi9O

[Uri Alon @UriAlonWeizmann]

When we came to see our destroyed labs hit by an Iranian missile, we found that a piano had survived
@WeizmannScience

[Elizabeth Vaisbourd @elizabethvaisb]

@plasmidsaurus Not sure if ad for @plasmidsaurus or Innsbruck university

[Uri Alon @UriAlonWeizmann]

Sweet moment in life, our 25th lab anniversary. In a circle, we looked at each other, a group of people with shared values and a soft approach, who want each other to succeed and be happy. (1/2)

[Elizabeth Vaisbourd @elizabethvaisb]

Had the chance to hear Chris Voigt present this work at a SynBio conference a few months back- absolutely jaw-dropping science. Huge congratulations to the team on this incredible project!

[Niko McCarty. @NikoMcCarty]

> bacteria were engineered to make molecules that absorb light in unique ways.
> the microbes were buried in soil.
> using a drone with a hyperspectral camera, one can "see" where the microbes are buried from ~300 feet away.

one of the most sci-fi papers I've seen in awhile.

[Theoretical Biophysics @TheoBiophysics]

I am putting some effort in keeping track of the conferences on Theoretical Biology and Quantitative Life Science. If you like to add some, please check this page. Help the community!

https://t.co/d4sgUu9U7p

[Olivier Borkowski @O_Borkowski]

Preventing plasmid multimer formation in commonly used synthetic biology plasmids
https://t.co/w9bK6XkZng

[Haotian Guo @HaotianGuo_qb]

Really cool!
1st thought - So basically we can have "multi-copy plasmid" by transforming a multimeric plasmid with BAC origin?
2nd thought - Jeez. How many clones in a combinatorial library may just be hetero-multimers?!! if they cannot be resolved later in recA strains...

[Elizabeth Vaisbourd @elizabethvaisb]

@HaotianGuo_qb remaining plasmid(s) sample, then transformation of a verified singlet and re-observing the phenotype.

[Elizabeth Vaisbourd @elizabethvaisb]

@HaotianGuo_qb 2. Regarding libraries, it depends on the strain they are built in. Cloning strains are surely a safer way to go. In any case (hetero)multimers are not necessarily inherited. In any case, double-checking hits after any library screen would be beneficial: first, by sequencing the

[Elizabeth Vaisbourd @elizabethvaisb]

Understanding multimer formation and using the MG1655 ΔrecA strain can significantly improve the design and reliability of synthetic biology experiments. Last but not least, shoutout to the team: David Glass,
@AnatBren and @UriAlonWeizmann

[Elizabeth Vaisbourd @elizabethvaisb]

Preventing plasmid multimer formation in commonly used synthetic biology plasmids
We survey and present a new way to quantify a hidden threat to synthetic biology - plasmid multimers. See the 🧵 for a tweetorial: 1/7

[bioRxiv Synthetic Biology @bioRxiv_synbio]

Preventing plasmid multimer formation in commonly used synthetic biology plasmids https://t.co/eSLHSrgTUt #biorxiv_synbio

[Elizabeth Vaisbourd @elizabethvaisb]

Deleting recombinases banishes multimers. The traditional cloning strain JM109 and a modified MG1655 ΔrecA strain prevent multimer formation. These are valuable tools for synthetic biologists to ensure reliable plasmid-based circuits. 6/7

[bioRxiv Synthetic Biology @bioRxiv_synbio]

Preventing plasmid multimer formation in commonly used synthetic biology plasmids https://t.co/eSLHSrgTUt #biorxiv_synbio