EuTRiPD

Network for European Training and Research in Peritoneal Dialysis

[Rebecca Herzog @RebeccaHerzog11]

ImprovePD Early Stage Researchers just nailed it in the PD-from bench to bed side session at  #ERA22 -  2 travel grants and best abstract by a young author award to Iva Marinović from Heidelberg (ESR2) 👏👩‍🔬🏆 #improvePD #MSCA

[Science Translational Medicine @ScienceTM]

Lithium chloride can protect mesothelial cells and mice from the toxic effects of #PeritonealDialysis fluids, suggesting the salt could be repurposed to prevent peritoneal #fibrosis and damage in patients. @MedUni_Wien @klauskratochwi1 @RebeccaHerzog11 https://t.co/W6LgAqaVhH

[CDL-MSRPD @cdl_msrpd]

Check out our new paper in this week's issue of @ScienceTM, on Lithium preserving peritoneal membrane integrity by suppressing mesothelial cell αB-crystallin... https://t.co/at7V1w9IY9 @ImprovePd @MedUni_Wien @RebeccaHerzog11

[Science Translational Medicine @ScienceTM]

Don't miss this week's new issue of Science Translational Medicine! https://t.co/oko1OfkdLN

[Rebecca Herzog @RebeccaHerzog11]

So if you are interested now, read the full paper here: https://t.co/h3qp7IWDkI 13/

[Rebecca Herzog @RebeccaHerzog11]

And… we made it to the cover! 🤯💪😎https://t.co/M2SLl1ysgD 12/

[Rebecca Herzog @RebeccaHerzog11]

So, can LiCl protect the peritoneum from PD fluid damage in vivo? In a chronic PD model in mice LiCl addition to PD fluid reduced peritoneal thickening, decreased CD31 positive cells in the mesentery, and also αB-crystallin and collagen in the parietal peritoneum. 9/

[Rebecca Herzog @RebeccaHerzog11]

In an adenoviral-mediated overexpression mouse model αB-crystallin induces peritoneal PD-like remodeling with increased vascularization and fibrosis-inducing SMAD3 phosphorylation. 8/

[Rebecca Herzog @RebeccaHerzog11]

But is it at all relevant in our PD patients? Looks like! Proteomics of effluent, IF of effluent cells, and IHC of biopsies of paediatric CKD and PD patients and healthy controls all show αB-crystallin abundance is elevated in peritoneal mesothelial cells of PD patients. 7/

[Rebecca Herzog @RebeccaHerzog11]

PDF-induced increase in VEGF release is regulated by αB-crystallin expression and LiCl prevents nuclear translocation of αB-c by inhibition of its and upstream phosphorylations, preventing its function to retain SMAD4 in the nucleus and reduce degradation, leading to fibrosis. 6/

[Rebecca Herzog @RebeccaHerzog11]

αB-crystallin (CRYAB) was the candidate protein up-regulated in all experiments and counter-regulated in both omics analyses and it was dose-dependently reduced by LiCl. Counter-regulation analysis (PD vs PD+LiCl) revealed consistent involvement of VEGF pathway signaling. 5/

[Rebecca Herzog @RebeccaHerzog11]

Following our concept of supplementation of PD fluids with cytoprotective additives, we found lithium chloride to improve cell survival of otherwise harmful icodextrin exposure. Repurposing drugs as additives offers accelerated, cost-effective clinical application. 4/

[Rebecca Herzog @RebeccaHerzog11]

They trigger fibrosis, vasculopathy, and inflammation. We show in a cross omics analysis that icodextrin fluids (used to spare glucose and reduce fluid overload), resemble cellular impairment known from glucose fluids – and therefore are not biologically inert. 3/

[Rebecca Herzog @RebeccaHerzog11]

Loss of kidney function affects millions from infants to geriatrics. Peritoneal dialysis is the cost-effective, home-based alternative to haemodialysis increasing health-related quality of life. But the used hyperosmotic fluids are the therapy’s Achilles’ heel. 2/

[Rebecca Herzog @RebeccaHerzog11]

Super excited to finally share our study reporting lithium supplementation of peritoneal dialysis fluid to prevent PD membrane deterioration, just published @ScienceTM #PeritonealDialysis #ImprovePD Highlights 👇, full paper: https://t.co/h3qp7IWDkI 1/14

[Klaus Kratochwill @klauskratochwi1]

@ImprovePd social evening... boiling hot science... is it the #EarlyStageReasearchers cooking for the principal investigators or vice versa?

[Klaus Kratochwill @klauskratochwi1]

Apply now: #PhD position in #H2020 @MSCActions ITN @ImprovePd - the #EarlyStageResearcher will have the chance to integrate #microbiome, #metabolomics and #proteomics data to provide better treatment for #kidneydisease patients on #PeritonealDialysis - https://t.co/9XSTZFuXq5

[MedUni Wien @MedUni_Wien]

Rebecca Herzog wins Stanley Shaldon Young #Investigator #Prize https://t.co/ACSXgt2oWy

[ERA - European Renal Association @ERAkidney]

Very wonderful talk and impressive research about Alanyl-Glutamine suplementation in PD Fluids by @RebeccaHerzog11 #ERAEDTA19 #peritonealdialysis

[Klaus Kratochwill @klauskratochwi1]

@RebeccaHerzog11 on "Alanyl-Glutamine supplementation in PD" at #ERAEDTA19