We have demonstrated that it is possible to use the optimal excitation wavelength for the calcium indicator without activating or depolarising cells. Furthermore, we imaged neuronal activity from single cells in brain slices whilst optically stimulating up to 20 Hz. (3/3)
Presenting the first all-optical, crosstalk-free activity readout using the calcium indicator CaSiR-1 in transgenic mouse brain slices expressing the opsin Chronos. (2/3)
1/9 We hypothesise that inhibitory microcircuits play an instructive role in adjusting stimulus representations during learning from brief reward signals. Here's a link to the pre-print: https://t.co/sYaGOKzAHA