FPbase

The Fluorescent Protein Database. tweets by @TalleyJLambert

[FPbase @FPbase]

Try the new FPbase spectra viewer!

Completely rewritten to be faster, more customizable, and full-featured:

• SVG/PNG/CSV export
• state recovery & URL sharing
• keyboard shortcuts for rapid entry 💚
• efficiency calculations
• full documentation

https://t.co/sG8FQHD0ye

[FPbase @FPbase]

Hi Fluorescence friends!

Please follow future updates on Bluesky:

https://t.co/D3nuYEyGCq

[FPbase @FPbase]

@joachimgoedhart @facette_lab @weinberz also commented in the thread by @christlet... but just an update that the names on FPbase have been updated keeping the conventions proposed in this thread here. With "mStayGold" (from Ando et al), StayGold-E138D, and mBaoJin

https://t.co/MnC46FuIUJ

[FPbase @FPbase]

@weinberz @joachimgoedhart @christlet Thanks @christlet for the helpful thread!

An update on the FPbase namings:

- "mStayGold" now refers to QC2-6 FIQ from Ando et al: https://t.co/JmMi1AAjJM
- Ivorra-Molla et al is "StayGold-E138D": https://t.co/sbzJxsm4Z0
- Piatkevich et al is mBaoJin https://t.co/kUqTm968af

[FPbase @FPbase]

@weinberz @joachimgoedhart @christlet They all retain the alias mStayGold, and so will all still show up when one searches for "mStayGold"

[FPbase @FPbase]

@weinberz @joachimgoedhart @christlet Thanks @christlet for the helpful thread!

An update on the FPbase namings:

- "mStayGold" now refers to QC2-6 FIQ from Ando et al: https://t.co/JmMi1AAjJM
- Ivorra-Molla et al is "StayGold-E138D": https://t.co/sbzJxsm4Z0
- Piatkevich et al is mBaoJin https://t.co/kUqTm968af

[James Manton @JamesDManton]

This exercise was less informative than I'd hoped, but perhaps it's interesting to someone else: spectroscopically-inferred estimates of densities of states for a selection of fluorescent proteins spanning the visible spectrum. Data from @FPbase.

[FPbase @FPbase]

recently hit 2,000,000 sessions on @FPbase ! 📈

[Ivan Maslov @IvanMasl0v]

Mutagenesis of #FbFP #LOV domain:

🟠 22 variants with 486-512 nm emission maxima

🟠 three-channel imaging via spectral unmixing

🟠 two-channel fluorescence lifetime unmixing

https://t.co/5Kgc045vlh

Congratulations, @ivangushchin, @Aynya5, and all the authors!

@FPbase

[FPbase @FPbase]

@bakermind (*not easily)

[FPbase @FPbase]

@bakermind took a look, there are 90 2P spectra currently available in the database. So, this is a user interface problem rather than a data storage problem 😬 ... (GM units are also stored in the database, but easily accessible) my apologies!

[Ben Campbell @lysergicdesigns]

Our article reporting hyperfolder YFP (#hfYFP) and other fixative-resistant fluorescent proteins based on #mGreenLantern is now available at @naturemethods. hfYFP enhances electron- and expansion-microscopies, protein purification, and so much more! 🧵https://t.co/hyUvgeno85

[FPbase @FPbase]

Chapter on using FPbase tools out now in the MMB issue on FPs. Thank you for editing @mayankdu!

[Mayank Sharma @mayankdu]

Happy to share the Methods in Molecular Biology issue on Fluorescent Proteins 🌈🎉😀. Including many “much awaited” protocols on and about Fluorescent Proteins/applications. A big THANKS to authors across the globe for contributing. 👏🏻💐
https://t.co/0yCqaDfzKw

[FPbase @FPbase]

@the_anatomist1 @aandr314 @helsinkiuni @Daniel_Wa19 Yes, but with more dyes, proteins, and filters, and no commercial interest 😉

[FPbase @FPbase]

@mycroscopy Wow, that’s a really impressive amount of organization! Very cool, congrats

[andrey a @aandr314]

this is genius: @helsinkiuni BioImaging Unit utilizes @FPbase to allow users test their fluorophores

https://t.co/GGDSgRJNHq

cc @Daniel_Wa19

[FPbase @FPbase]

@fungalhyphae @aandr314 @helsinkiuni @Daniel_Wa19 Love it! 😍

[FPbase @FPbase]

anyone out there routinely express FPs in Chlamydomonas? any modern suggestions for SNR? (presumably red, but any expression gotchas?)

[FPbase @FPbase]

@beniroquai That would be 50% of max Extinction coefficient (not QE). If you’re asking whether it “works”, it will be case-specific. Will require more illum power, so more damaging to sample. And may generate more background, depending on sample. But with enough power you’ll get photons :)