Top Tweets for #CASPAM
🔥Featured in @ACS_Sensors🔥#CASPAM is a triple-modality reporter for #multiplexed #imaging of #caspase activity. By means of #Fluorescence #Polarization #Anisotropy, #CASPAM enables to simultaneously image the activity of three different nodes of the apoptotic network #homoFRET
#Caspase activity #sensor by polarization anisotropy #multiplexing a single-plasmid triple-modality reporter of key nodes of the #apoptotic network to analyze #molecular pathways! #FRET #biosensor #monitoring @UBAonline. https://t.co/0oKc8tHPNZ
As we’ve recently shown, knowing the relative concentration between biosensors achieved with #CASPAM allows for multiplexed experiments with lower variation in expression levels, and thus enables for highly improved data-driven modelling. https://t.co/sBoxL9IhM0
By using #CASPAM and thus achieving near #equimolar stoichiometry in the expressed homo-FRET #caspase activity biosensors, we showed that perturbations to the experimental system are much better controlled, leading to substantially less variation in biological observables
#CASPAM approach enabled #equimolar biosensor co-expression. By using Fluorescence Correlation Spectroscopy (#FCS) in living cells, we showed that #CASPAM can successfully be used for tightly controlled, #ratiometric caspase activity biosensor co-expression in individual cells
Conversely to the widely varying cell-to-cell expression ratios achieved in conventional co-expression strategies, #biosensor #coexpression from #CASPAM takes place at a defined ratio, remaining virtually constant within the entire dynamic range
High hydrolytic efficiency for the viral 2A sequences cleavage, #coexpression, and proper folding of the single #homoFRET #biosensors was successfully achieved from a single ORF in the #CASPAM arrangement
#homoFRET #biosensors rely on anisotropy changes to assess protease activity. By using viral 2A self-cleaving sequences #CASPAM was engineered as a single plasmid, enabling simultaneous expression of three homo-FRET biosensors for co-imaging the activity of caspases 3, 8, and 9
Happy to share our most recent publication in @ACS_Sensors. We report the development and full characterization of #CASPAM, a single plasmid-based triple-modality system for #multiplexed #imaging of #Caspase network activity in #Apoptosis. https://t.co/eYHrCT9mc7
@WomenDigitalNTW #CASPAM conference at @Acmeworks : The act of asking for consent to send commercial #Emails is considered a #CEM.
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