Top Tweets for #DAPI
🚀 BUILD ON DASH ⚡️
Ready to build decentralized applications on Dash Platform?
Explore Identity, Data Contracts, Documents, DAPI, Tokens and more with the Dash SDK and developer tools.
#Dash #DashPay #DashDaoTH #DashDaoCenter
Need #fluorescence staining but unsure which #dye fits your experiment? 🤔
#DAPI, #Hoechst, and #FITC are all widely used fluorescent dyes—but each serves a different purpose depending on your sample and imaging setup.
🔬 DAPI & Hoechst — Nuclear DNA stains
Both bind strongly to DNA (A-T rich regions) for clear nuclear visualization.
• DAPI: Best for fixed/permeabilized cells, UV/405 nm excitation
• Hoechst: Cell-permeable, ideal for live-cell imaging
👉 Use for nuclear morphology, cell counting, cell cycle analysis
🧫 FITC — Protein & antibody labeling
A classic green fluorophore for immunofluorescence and flow cytometry.
• Bright signal when conjugated to antibodies/proteins
• Note: can photobleach—Alexa Fluor 488 is a more stable alternative
👉 Use for target protein detection and localization
💡 Quick guide
Nuclei → DAPI / Hoechst
Proteins → FITC (or modern substitutes)
Multicolor imaging → plan carefully to avoid spectral overlap
At MedChemExpress (MCE), we offer 5,000+ fluorescent probes and dyes to support a wide range of imaging applications, with high sensitivity and strong signal stability. https://t.co/DlVBDcgWk7
#Microscopy #CellBiology #LabLife #Immunofluorescence #Biotech
蛍光染色（#fluorescence staining）に使う蛍光色素、どれを選べばいいか迷っていませんか？🤔
#DAPI、#Hoechst、#FITCはいずれも広く使われる蛍光色素ですが、それぞれ用途やサンプル条件に違いがあります。
🔬DAPI & Hoechst — 核DNA染色
どちらもDNA（特にA-Tリッチ領域）に強く結合し、核構造を明瞭に可視化できます。
• DAPI：固定・透過処理済み細胞に最適、UV/405 nm励起
• Hoechst：細胞膜透過性があり、生細胞イメージングに適用可能
👉 核形態観察、細胞数カウント、細胞周期解析に使用
🧫FITC — タンパク質・抗体標識
免疫蛍光やフローサイトメトリーで定番の緑色蛍光プローブ。
• 抗体やタンパク質に結合すると強い蛍光シグナルを発する
• 注意：光退色しやすいため、より安定なAlexa Fluor 488が代替として使用されることも
👉 標的タンパク質の検出・局在解析に使用
💡クイックガイド
核 → DAPI / Hoechst
タンパク質 → FITC（または改良型蛍光色素）
多重染色 → スペクトル重なりを避ける設計が重要
MedChemExpress（MCE）では、幅広いイメージング用途をサポートする5,000種類以上の蛍光プローブ・色素を提供しています。高感度かつ安定したシグナルで研究を支援します。https://t.co/38GeI27xfq
#蛍光染色 #蛍光イメージング #細胞生物学 #免疫蛍光 #顕微鏡 #実験室研究 #フローサイトメトリー #分子生物学
❓Tech Q&A | Can #DAPI and H₂DCFDA be used together for ROS staining? Is fixation required?
• Yes — DAPI can be combined with H₂DCFDA as a nuclear counterstain; excitation/emission are well separated and no major interference is expected
• Live-cell imaging preferred — PFA fixation may alter #ROS distribution and reduce signal accuracy
• Before imaging, wash with PBS or phenol red–free medium
• Use phenol red–free medium for imaging to reduce background
Refs: PMID: 34652919; 39235087
#ROS #CellImaging #Fluorescence #TechQA
Sempre contenuti di alto livello 🫡
@Dashpay @DigitalGoldTalk
$Dash #Dash #DAPI #Decentralization #Freedom
On Dash there's something called Decentralized API (DAPI) that lets you access the whole network without running a full node yourself. @TheDesertLynx
Clip from today's @DigitalGoldTalk chill session
When pigs drink moonshine, they turn into tanks. But tanks still explode.
Drone Attack: Pig Irruption drops you into a battlefield of chaos, where every shot counts and the difficulty is — yes — over-deadly.
Destroy stills. Flatten trucks. Survive if you can. #DroneAttack #DAPI
Explosions, chaos, and bacon rain from the skies in Drone Attack: Pig Irruption.
If you think you’ve played hard games, think again — this one is over-deadly.
Warning: highly addictive. May cause a thirst for total destruction. #DroneAttack #DAPI #Drone #Pigs #Truck #IndieGame
Ever get the urge to obliterate cartoon pigs from the sky?
Us too.
🚁 Drone Attack: Pig Irruption
🧨 Explosions
🎮 Smooth controls
🧽 Moral ambiguity
Get your pixel bloodlust fix now.
#DroneAttack #MobileGaming #IndieDev #GameRec #CartoonShooter #DAPI #Drone #IndieGame #Godot
Looks like #DAPI just gave a masterclass in speechless gains, bros —5500% rise and all I can say is *WOW*! Guess silence really is golden
https://t.co/gHicFcCokM
BqnLFVhfjJyriYJtjSb9jpkjZbWBPBxXKXm2ArN5pump
🌲 Update 1.4.4 is here!
✔️ New tree & bush graphics
✔️ Move and switch weapons at once
🐞 Bug fixes included
🔫 Time to wreak havoc — better, faster, and greener than ever.
#indiedev #mobilegame #update #gamedev #DroneAttack #DAPI #Trees #Bushes #Drone #IndieGame #GodotEngine
Over Deadly difficulty turns up the heat! 💀 Combat mosquito deploys slime for the perfect trap, obliterates a truck 💥, and wipes out pigs in one brutal sweep 🐷🔥. Chaos has never looked so tactical. #DroneAttack #DAPI #Truck #Drone #Mosquito #Pigs #IndieGame #Godot #IndieDev
On Over Deadly difficulty, the combat mosquito sends explosive gifts straight through the chimney 🎁💥—wet pigs, shocked faces, and glorious chaos in the water! 🐷💣🚁 #DroneAttack #DAPI #Drone #Mosquito #IndieGame #Godot #IndieDev #Pigs #Pink #Bacon #GodotEngine #Attack #Chimney
On Over Deadly difficulty, the combat mosquito unleashes destruction — obliterating pigs and smashing through the truck convoy with style and firepower! 🚁💥🐷 #DroneAttack #DAPI #Drone #Mosquito #Truck #Pigs #Pink #Bacon #IndieGame #Godot #IndieDev #GodotEngine #CombatMosquito
On Over Deadly difficulty, the combat mosquito restores full health and unleashes mass destruction on waves of pigs! Non-stop carnage, total domination! #DroneAttack #DAPI #Drone #Mosquito #Truck #Pigs #Pink #Bacon #Godot #IndieGame #IndieDev #GodotEngine #Attack #Fun #Explosion
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