Top Tweets for #FLAMseq

almost 7 years ago

Nuevos metodos para #RNAseq de mensajeros completos incluidos las colas de #poliA #FLAMseq #genomica #ciencia #RNA - FLAM-seq: full-length mRNA sequencing reveals principles of poly(A) tail length control https://t.co/5l4QWaH8Wo

Ivano Legnini @ILegnini

almost 7 years ago

8/10. We find quite some Cs in the tails (other labs found regulatory Us and Gs at the 3’ end of the tails, but before #FLAMseq we couldn’t really sequence through the entire tails). We don’t really know what they do there.

Ivano Legnini @ILegnini

almost 7 years ago

3/10. What is it about? We combined long-read sequencing with a new strategy for cDNA library prep, enabling to sequence entire mRNAs, including their entire poly(A) tail. We thoroughly validated #FLAMseq with PAT assays, northerns and several comparisons with published data.

Ivano Legnini @ILegnini

almost 7 years ago

1/10. Our #FLAMseq paper is finally out on @naturemethods! If you’re interested in #RNA #polyAtails, #generegulation and #longreads with #PacBio, this thread is for you. The paper is here: https://t.co/sXuf1JQWnn

Ivano Legnini @ILegnini

about 7 years ago

If you're interested in #longreadsequencing of #mRNAs and #polyAtails come to my talk, the first in session 10 on new research trends and technologies, at 2.30 in the theatre hall! #FLAMseq #RNA2019

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