Top Tweets for #LocalizedReconstruction
Where did viruses come from? See our #preprint "New Vista into Origins of Viruses from a Prototypic ssDNA Phage" #cryoEM #LocalizedReconstruction by @elaanto @VAbrishami @M__Selvaraj @sundberglr and others https://t.co/dYFm6ZeBDn
If you’re working on #cryoEM of flexible complexes, this tutorial on #LocalizedReconstruction in @scipion_em maybe useful.
@pnmshah @biochem_fan Sounds interesting and thanks for testing! Note that #LocalizedReconstruction (now a plugin in Scipion) and the new symmetry relax option in Relion are two different things (but they can be combined like we did for adeno fiber)
It makes us very happy to see #LocalizedReconstruction being used! We are currently preparing a tutorial with @VAbrishami for this new version. Watch this space!
We have added our #LocalizedReconstruction method for #cryoEM data analysis of flexible structures in @scipion_em. The method improved the resolution of human adenovirus components. @VAbrishami @SerbanIlca @delarosatrevin @JM_Carazo https://t.co/31vEUyXrsN
@biochem_fan Symmetry expansion, focused classification and #localizedreconstruction all have their uses. This new RELION option for symmetry relaxation can help with pseudo-symmetries or when there is one single symmetry mismatch. @SerbanIlca @VAbrishami
@JeremyKeown1 @OxfordStrubi @jonathan_grimes @CarriqueLoic @Itziar_Serna @AbhayKot @SerbanIlca Nice work, congratulations everyone! Also a nice demonstration how #LocalizedReconstruction helps dealing with flexibility in large viruses.
Announcing that our long awaited story on clathrin structure is out in @NatureSMB today. Multiple #clathrin architectures determined by #CryoEM. If you like heterogeneity, this one is for you. The structure of the triskelion hub was determined using #LocalizedReconstruction.
Multiple liquid crystalline geometries of highly compacted viral nucleic acid unveiled by #cryoEM. A real tour-de-force by @SerbanIlca using #LocalizedReconstruction. Thank you @wellcometrust @SuomenAkatemia @HiLIFE_helsinki @SigridJuselius @OxfordStrubi https://t.co/t34XtobwWd
@HeskethEmma @BJ_Greber @DTegunov @naranson @Lomonossoff_Lab @Tomlinson_lab @SciReports If you upload the data to @EMDB_EMPIAR I promise to give it a go using #localizedReconstruction ☺️
This paper is a nice demonstration how #localizedReconstruction helps to improve #cryoEM maps of large complexes and their flexible/symmetry-mismatch structures - in this case the horn-shaped structures at the capsid vertices
Finally published:
Assembly of complex viruses exemplified by a halophilic euryarchaeal virus
https://t.co/JnSQlOW35w
We got beautiful crystals which did not diffract that well. Luckily development of cryo-EM has taken huge steps in recent years.
The raw #CryoEM data from our recent @CellCellPress paper on AP-1:Arf1:Tetherin-HIV-Nef. The entries include all particles, the trimer subset and the #LocalizedReconstruction extracted trimer monomeric subunit. https://t.co/Ac4CeZLPT4
I am delighted to announce our paper released today in @CellCellPress. AP-1 is a molecular gymnast, it makes its poses depending on the cargo it binds and #HIV Nef performs with it to subvert antiviral cargo trafficking. #CryoEM #LocalizedReconstruction https://t.co/hUmEwdVZu0
Welcome to Twitter @VAbrishami and my group @HiLIFE_helsinki. Vahid will be working on computational methods for #cryoEM including #LocalizedReconstruction
Great to see #LocalizedReconstruction methods improving the resolution in large virus capsids https://t.co/nh3ydSAdE7
On my way to @scilifelab course on #CryoEM to teach #LocalizedReconstruction and other advanced methods with @delarosatrevin
#cryoEM combined with our #LocalizedReconstruction method reveals viral RGD-loop interaction with #integrin receptor https://t.co/OGB89JHZz0
#CryoEM structure of human #adenovirus D26 including #LocalizedReconstruction of the fiber by Yu et al https://t.co/vAtHjKdEXw
Our #LocalizedReconstruction method is now fully functional in #ScipionEM. Thank you @scipion_em team!
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