Top Tweets for #UniqueMolecularIdentifiers
A team led by @envbio of @UWDEOHS @uw_cee @UW developed a targeted, long-read sequencing method utilizing #UniqueMolecularIdentifiers to generate accurate, full length CTX-M gene sequences from #WasteWater. #AntimicrobialResistance
ES&T Letters: https://t.co/TJc2h6nzZv
#cellCounts employs the seed-and-vote strategy to align reads to a reference #genome, collapses reads to UMIs (#UniqueMolecularIdentifiers) and then assigns UMIs to #genes based on the featureCounts program. - @latrobe https://t.co/r00jaHQPv0
Researchers from the @karolinskainst introduce molecular spikes—#RNA spike-ins containing built-in #uniquemolecularidentifiers that they use to identify critical experimental and computational conditions for accurate RNA counting in #singlecell #RNAseq. https://t.co/8PU4p1LaeL
Smart-seq-total does not require splitting the #RNA content of a cell and allows the incorporation of #uniquemolecularidentifiers into short and long RNA molecules for absolute quantification. - @Stanford - @StephenQuake https://t.co/AMPjHQkaWL
Fact and Fiction about 1%: Next Generation Sequencing and the Detection of Minor Drug Resistant Variants in HIV-1 Populations with and without #UniqueMolecularIdentifiers. View Full-Text: https://t.co/LzvFFPE5cJ
#drugresistancemutations; #uniquemolecularidentifier; #primerID
For #scRNAseq data lacking #uniquemolecularidentifiers (UMIs), @PrincetonCS researchers propose #quasiUMIs: quantile normalization of read counts to a compound Poisson distribution empirically derived from UMI datasets. - @Princeton https://t.co/Bjmm44cNWJ
While #PCR amplification bias is often taken care by use of #UniqueMolecularIdentifiers (UMIs), normalization is required to remove effects of other technical variations like differences in #sequencingdepth, cell lysis and #reversetranscription efficiency https://t.co/uzRIbOc9ep
#zUMIs - A fast and flexible pipeline to process #RNAsequencing data with #uniquemolecularidentifiers (UMIs) - https://t.co/kYJ24HTcqT - @LMU_Muenchen
Inc of #uniquemolecularidentifiers in #TruSeq adapters improves the accuracy of quantitative #sequencing - https://t.co/3QoHsGSAuu - @NYUCAS
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