Jafar Jabbari

Nanopore, PacBio, Illumina and Single Cell Genomics Platforms Technologies Developer, Microbiomics, Meta-omics, Genomics Innovation and Development Scientist

[Raymond Yip @rkhyip]

Excited to share our teamwork on spatially profiling human bone marrow trephines is now online! 🔬🗺️🦴
https://t.co/85kSWG6PTE
More high-res atlases to come - liquid/solid cancer in bone, cellular therapies settings..💪
#Xenium #myeloma #spatialomics

[Jafar Jabbari @jafarjabbari]

@LGMartelotto Can the cost per detected gene, as opposed to per cell or per sample, be compared between this imaging-based and sequencing-based methods?"

[Jafar Jabbari @jafarjabbari]

Are you using separate assays to measure transduction efficiency, vector copy number, and integration sites?

Our high-throughput, droplet-based single-cell method streamlines all three analyses into a single assay.

Visit poster #159 today at #LorneGenome25 to learn more!

[Daniel V Brown @dvbro0]

Tired of tedious RNA extractions for your transcriptomic studies? Introducing TIRE-seq, a novel protocol that streamlines bulk RNA-seq by integrating sample extraction with library preparation. The whole process can be completed in a day and a half. (1/6) https://t.co/kvrTlLGvFA

[Jafar Jabbari @jafarjabbari]

@Psy_Fer_ MiSeq i100 (cheaper version) is~$49k. It only can use 5M and 25M kits. It seem that Illumina is keen to trade in old MiSeqs.

[Jafar Jabbari @jafarjabbari]

@AlenZimic @nanopore It is bulk cDNA enriched for some target genes. The average library fragment size is 2.2Kb.

[Jafar Jabbari @jafarjabbari]

1 pION flow cell, 1 load, no wash, 2 runs because pores looked good after completion of 1st run and around 200GB estimated output.
I want more flow cells like this! @nanopore

[Jafar Jabbari @jafarjabbari]

@JLGONZHER @nanopore 60 fmol loading, 21 target enriched cDNA samples barcoded with native barcoding kit

[Jafar Jabbari @jafarjabbari]

@gaworj @nanopore It is run on 23.11.7

[Jafar Jabbari @jafarjabbari]

@OWallerman @nanopore 8800 pores

[Jafar Jabbari @jafarjabbari]

@TJesse62 @nanopore Is there a picture of how they fit?

[Xueyi Dong 董雪漪 @DongXueyi]

Our long read benchmarking work is finally published🥳

[Jafar Jabbari @jafarjabbari]

@nanopore good day with R9. Run barcoded PCR-cDNA library for 3 days. It was sitting on sequencer over weekend. On Monday found that there are 3K live pores. Started a 2nd run without washing or reloading. Yielded 9GB data totaling 124GB. A personal record with PCB111.24 library.

[Jafar Jabbari @jafarjabbari]

@isidrolauscher @NatureBiotech @fran_muyas I wonder if the pipeline can handle nanopore reads from single cell full-length cDNA and ATAC-seq as well.

[Jafar Jabbari @jafarjabbari]

James! Your impressive multidisciplinary approach, integrating metabolomics, single-cell genomics, and more, is outstanding!

[Jafar Jabbari @jafarjabbari]

Thanks for invitation and audience engagement.

[South Australian Genomics Centre (SAGC) @SA_genomics]

Great @SA_genomics event last night with @nanopore @UniofAdelaide. A huge THANK YOU to our speakers (@CristobalOnetto, @jafarjabbari and Dr. Kevin Fenix) for telling us about their research. And thank you to everyone who attended.

[Jafar Jabbari @jafarjabbari]

@AliciaOshlack @agtaGenomics @ResAustralia @mritchieau Congratulations!🍾

[Jafar Jabbari @jafarjabbari]

@adbeggs @TJesse62 @nanopore @JoStockton1 @beggs_lab I wonder what you mean by conventional WGA? There are many WGA methods.

[John Atack @sciency_atack]

all made possible thanks to great collaborators @julienisking @greg_tram @jafarjabbari and many others from across the globe