Okamura Lab

奈良先端科学技術大学院大学•RNA分子医科学研究室です。RNA Molecular Medicine Laboratory at NAIST, Japan.

[Okamura Lab @lab_okamura]

The Knock-in atlas server is back online now. Thank you for your understanding!

[Okamura Lab @lab_okamura]

The Knock-in Atlas server (https://t.co/4Vq3xY6Uxi) will be down for maintenance:
From: March 24, 5:00 PM (JST)
To: March 26, 12:00 PM (JST)
Sorry for the inconvenience!

[Okamura Lab @lab_okamura]

And, for those of you who are interested in making gRNAs against gene introns for NHEJ-mediated knock-in, we also made lists guidelines and genome-wide lists of gRNAs for human and mouse. For more details, please visit https://t.co/4Vq3xY6Uxi

[Okamura Lab @lab_okamura]

knock-in atlas: a web resource for targeted protein trap by CRISPR/Cas9 in human and mouse cell lines https://t.co/HUKJWk7nP5 Our knock-in atlas paper is out! The collection has >600 entries for 350 proteins (239 RNA binding proteins) tagged with the Venus fluorescent protein

[Okamura Lab @lab_okamura]

knock-in atlas: a web resource for targeted protein trap by CRISPR/Cas9 in human and mouse cell lines https://t.co/HUKJWk7nP5 Our knock-in atlas paper is out! The collection has >600 entries for 350 proteins (239 RNA binding proteins) tagged with the Venus fluorescent protein

[Okamura Lab @lab_okamura]

The site is back online now!

[Okamura Lab @lab_okamura]

The knock-in atlas website <https://t.co/4Vq3xY6Uxi> will be down soon until Monday October 20th morning (JST). Sorry for the inconvenience!

[Okamura Lab @lab_okamura]

The knock-in atlas website <https://t.co/4Vq3xY6Uxi> will be down soon until Monday October 20th morning (JST). Sorry for the inconvenience!

[Okamura Lab @lab_okamura]

A new version of knock-in Atlas is available now at bioRxiv! The collection has now grown to 350 proteins https://t.co/ecXMdRUKXP

[Okamura Lab @lab_okamura]

Want to tag a protein in cell lines? Check out our new preprint and website <https://t.co/5Osk3fK0eM>. We made a catalogue of gRNAs against human/mouse introns for inserting artificial exons for protein tagging at endogenous loci.

[Okamura Lab @lab_okamura]

新入生の皆さん、新任の教職員の皆さん、NAISTへようこそ！

[Okamura Lab @lab_okamura]

春のインターンシップ生の中尾さんの最終日が明日となりました。どうもありがとう！下田さんはあと一週間ほど、まだよろしくお願いします！花井さん、ご指導感謝します！

[Okamura Lab @lab_okamura]

@Mvsa_Nas Congrats, Musa!

[Okamura Lab @lab_okamura]

第5回関西RNAクラブ、皆様のご参加ありがとうございました。活発な交流の場となりました！奈良先端RNA分子医科学研究室の皆さん、準備や運営ありがとうございました（写真に写っていませんが、特に飯田さん、お疲れ様でした！）。

[Okamura Lab @lab_okamura]

明日の関西RNAクラブ、皆様のご参加楽しみにしています！奈良先端大キャンパスもちょっとだけ春らしくなってきました。明日も暖かいこと願っています！

[Okamura Lab @lab_okamura]

明日の関西RNAクラブ、皆様のご参加楽しみにしています！奈良先端大キャンパスもちょっとだけ春らしくなってきました。明日も暖かいこと願っています！

[Okamura Lab @lab_okamura]

3月14日奈良先端大で第5回関西RNAクラブ開催です。皆様のご参加お待ちしております！https://t.co/1prSgVxN63

[Mishima Lab @MishimaLab]

博士研究員の募集をJREC-INに掲載しました。ゼブラフィッシュをモデルとしたRNA研究に興味がある方、プロジェクトや条件面の詳細を知りたい方は、三嶋まで気軽にお問い合わせください。
https://t.co/rFTe67EgLJ

[Okamura Lab @lab_okamura]

In the mouse and human gRNA sites, you can search for your genes of interest. The gRNA scores and AlphaFold2 pLDDT scores around the tag-insertion sites are useful for finding good gRNAs (Regions with low pLDDT are preferred as such regions tend not to have tight structures.)

[Okamura Lab @lab_okamura]

Want to tag a protein in cell lines? Check out our new preprint and website <https://t.co/5Osk3fK0eM>. We made a catalogue of gRNAs against human/mouse introns for inserting artificial exons for protein tagging at endogenous loci.

[bioRxiv Molecular Biology @biorxiv_molbio]

The Knock-In Atlas: A web resource for targeted protein trap by CRISPR/Cas9 in human and mouse cell lines https://t.co/QyWJDoGzg0 #biorxiv_molbio

[Okamura Lab @lab_okamura]

You can also find confocal Images, flow-cytometry data, Western blot results of knock-in HEK293T lines for 87 genes by clicking the "Go to Collection page" button.

[Okamura Lab @lab_okamura]

3月14日奈良先端大で第5回関西RNAクラブ開催です。皆様のご参加お待ちしております！https://t.co/1prSgVxN63

[Okamura Lab @lab_okamura]

Congratulations Li Ze for the publication of her PhD work at NAIST! Her findings explain how flies export tRNAs without the canonical export factor Exportin-t.
Exportin-5 binding precedes 5’- and 3’-end processing of tRNA precurso... https://t.co/YdFjXIbkAc

[Okamura Lab @lab_okamura]

We have a vacant assistant professor position. Please apply if you are interested in studying RNA biology in an environment where you would have a plenty of opportunities to work with graduate students. https://t.co/5xQBoNEDju

[Okamura Lab @lab_okamura]

多くの大学院生の指導をしつつRNA生物学研究に没頭したい方、本研究室の助教ポジションにご応募ください！https://t.co/vQ1tGJgMn3