@TheCoagMavrick I plunge froze with Vitrobot: 3.5 ul sample volume, -10 Blot force, 6 seconds blot time. The chamber was cooled to 4°C with 95% humidity. ❄️
Take a look at a sketch by physics laureate Ernst Ruska, dated 9 March 1931, of the cathode ray tube for testing one-stage and two-stage electron-optical imaging by means of two magnetic electron lenses (electron microscope). Ruska was awarded the 1986 physics prize for his work.
@danielgomezMCB @SachseGroup Yes, posters can be very helpful in reducing the delivered information to the most important highlights of a project. In this case the ribosomes were free. 😀
After 2.5 years of my PhD, I presented my first printed poster today. Good Bye virtual meetings and online poster presentations, I'll only miss you a little bit. #cryoEM#ribosomes@SachseGroup
New research from our lab! We elucidated how Atg18 oligomers form structural scaffolds for membrane interaction. Lots of #CryoEM structures (Helical assemblies, SPA, subtomo) suggest a conserved interaction motif for Atg18-Atg2
https://t.co/hV802ANKJc