Medical Affairs - MicroGenDX

Medical & Scientific Division of MicroGenDX with the goal of disseminating technical information & the vision of increasing the clinical decisions driven by NGS

[Medical Affairs - MicroGenDX @ma_microgendx]

Published this month in the Canadian Journal of Urology, Chung et al. demonstrate that NGS outperforms culture, showing more diversity and richness than previously thought, in patients undergoing penile prosthesis revision surgery.

https://t.co/6CiWh04UXs

[Medical Affairs - MicroGenDX @ma_microgendx]

We wish you a Merry Christmas!🎄

Have a magical day with your family and loved ones🤍

[Medical Affairs - MicroGenDX @ma_microgendx]

#BehindTheSequencing

Since mold cultures may take 1-4 weeks of incubation and requires highly skilled microscopic identification, ITS NGS can identify fungi (yeasts and molds) in less than 5 days from a fungal culture or directly from a clinical/patient sample.

[Medical Affairs - MicroGenDX @ma_microgendx]

How can NGS provide unbiased detection of fungal species? The Internal Transcribed Spacer (ITS) region in the ribosomal RNA operon is conserved across fungal genomes; these conserved regions serve as primer binding sites for the amplification of the intervening sequence.

[Medical Affairs - MicroGenDX @ma_microgendx]

#BehindTheSequencing

MicroGenDX laboratory is supported by a team of molecular biologists, biochemists, bioinformaticians, computer scientists, and physicians. MicroGenDX employs over 200 passionate employees with a leadership model that swiftly adapts to global health concerns.

[Medical Affairs - MicroGenDX @ma_microgendx]

Filling diagnostic gaps with NGS gives healthcare professionals the entire picture of the infection. It allows targeted therapies to help slow the rapid development of antimicrobial resistance and preserve a few of our already dwindling treatment options.

[Medical Affairs - MicroGenDX @ma_microgendx]

#WorldAntimicrobialAwarenessWeek

Resistance is characterized by mutations that arise under the selective pressure of antibiotics whereas antibiotic tolerance is an increased resilience to antibiotic treatment in a non-specific way.

A thread

[Medical Affairs - MicroGenDX @ma_microgendx]

Biofilms and tolerance are difficult to detect in traditional cultures, and without all the information, we may still be inappropriately utilizing antibiotics and contributing to the development of resistance.

[Medical Affairs - MicroGenDX @ma_microgendx]

For example, shutting down many metabolic pathways in the persister or VBNC (Viable but not culturable) state, thus disrupting the antibiotic’s mechanism of action.

[Medical Affairs - MicroGenDX @ma_microgendx]

#WorldAntimicrobialAwarenessWeek

A 35-old woman with type I diabetes had a large transplant surgery abdominal wound open for months. The abscess culture showed no growth. The same fluid was sent to MicroGenDX for NGS, which detected Rhizopus in 4 days.
https://t.co/A58LLCom4G

[Medical Affairs - MicroGenDX @ma_microgendx]

Is the evaluation and treatment of the vaginal microbiome part of your comprehensive management of cervical pathologies?
#obgyn #cervicalmicrobiome #womenhealth #hpv #Gynecology #microbiology

[Medical Affairs - MicroGenDX @ma_microgendx]

It is essential to understand the impact the vaginal microbiome exerts over the atypical changes seen in cervical dysplasia due to HPV infection. A proper comprehensive microbiome analysis and intervention should be part of the evaluation. https://t.co/zkKObRmpff

[Medical Affairs - MicroGenDX @ma_microgendx]

Following last night's opening keynote delivered by @jmGhigoLab of the Institut Pasteur, we are very excited about the biofilm research being presented the rest of this week at the @ASMBioFilms in Charlotte, N.C.

Visit the MicroGenDX/RTL Genomics booth this week.

[Medical Affairs - MicroGenDX @ma_microgendx]

In an infective endocarditis (IE) study from 44 patients’ resected heart valves that compared NGS, blood culture, and valve Gram staining, NGS proved to be faster and more sensitive with a better negative predictive value (NPV) than the other two methods.
https://t.co/WEJwU5Yj8y

[Medical Affairs - MicroGenDX @ma_microgendx]

A recent prospective study conducted at 14 institutions published in the Journal of Bone & Joint Surgery defines the NGS profile of 85 culture-negative PJI cases that met the ICM criteria, with NGS detecting organisms in 65.9% of culture-negative cases

https://t.co/LSAERwPbN9

[Medical Affairs - MicroGenDX @ma_microgendx]

This prospective multicenter study involved 13 academic institutions. NGS detected several organisms in most positive samples. However, in most infected patients, 1 or 2 organisms were dominant.

Link to abstract presented in AAHKS 2018: https://t.co/94uQ5QXJXp

[Medical Affairs - MicroGenDX @ma_microgendx]

Surgical management of PJI remains challenging with patients failing treatment despite our best efforts. An important question is whether these later failures reflect reinfection or the persistence of infection. Treatment failure was defined using a modified Delphi criterion.

[Medical Affairs - MicroGenDX @ma_microgendx]

Is your reference database up-to-date? NGS can simplify diagnostic workups.

Elizabethkingia spp.
These Gram-negative rod-shaped bacteria are associated with life-threatening infections. Accurate speciation of this genus is necessary for the diagnosis and management.

[Medical Affairs - MicroGenDX @ma_microgendx]

#BehindTheSequencing

Next Generation Sequencing vs. PCR:

[Medical Affairs - MicroGenDX @ma_microgendx]

#TheNewDiagnosticEra

From Pasteur to NGS technology: steps towards precision medicine

Next-generation sequencing technologies have been developed at the onset of the 21st century. These technologies provide a highly efficient, rapid, and low-cost DNA sequencing platform.