Mariano Gonzalez Pisfil

FLIM guy at the Core Facility Bioimaging #BMC @LMU_Muenchen. In the little-big world of fluorescence lifetime. Self-proclaimed dancer in my free time.

[EMBL Events @EMBLEvents]

👋Hello from day two of the EMBL course 'Fluorescence imaging beyond intensity' at the EMBL Imaging Centre.
#EMBLFluorescenceLifetime

Participants enjoying a good coffee and conversations between the lectures by Birgit Koch, @mgpisfil and @jalink_kees. ☕🤝

[EMBL Events @EMBLEvents]

Welcome to the first day of #EMBLSuperResolution! Participants can look forward to gaining in-depth knowledge on all aspects of STED Nanoscopy 🔬
Co-organised with @LeicaMicro, it's bound to be a great course 💪

[Luis AJ Alvarez @L_AJ_Alvarez]

Prof. Dorus Gadella discussing Fluorescence Proteins for nanoscopy imaging on the EMBl STED course #EMBLSuperResolution. Stunning developments in FPs 🤩!

[Luis AJ Alvarez @L_AJ_Alvarez]

@ChristelPoujol & Magali Grison from @BIC_Bordeaux running a hands on session in TauSTED in plants with both multicolour fixed but also live plants looking at Golgi organisation #EMBLSuperResolution

[EMBL Events @EMBLEvents]

It's Day 3 at #EMBLSuperResolution and we've already covered so much ground! Today's topics included 'Combining STED and expansion microscopy', 'Investigating the structure and dynamics of mitochondria at the nanoscale', and more 🔬

[Luis AJ Alvarez @L_AJ_Alvarez]

@mgpisfil Mariano showing us the ways of STED multiplexing leveraging fluorescence lifetime and spectral flexibility 🤩 #EMBLSuperResolution

[Mariano Gonzalez Pisfil @mgpisfil]

#EMBLSuperResolution

[Mariano Gonzalez Pisfil @mgpisfil]

Time-resolved STED nanoscopy course at @embl is starting!

[Mariano Gonzalez Pisfil @mgpisfil]

Thanks to you @alesposito75 for your visit. Great talks and great workshops! A pleasure to chat with you about the state of the FLIM world.

[Alessandro Esposito 👨‍🔬🔬🧬👨‍👩‍👧🇪🇺🏳️‍🌈 @alesposito75]

A massive thanks to Steffen Dietzel and @mgpisfil for the organisation and invite and all those that reached out to chat about science and technology

[Ali Gheisari @QeisariAli]

2nd day and more #FLIM talks
@alesposito75 discussing live single cell FLIM imaging.

#FLIMSym

[Mariano Gonzalez Pisfil @mgpisfil]

@QeisariAli Thanks @QeisariAli for the introduction!! Great way to end the day!

[Ali Gheisari @QeisariAli]

every microscopic is at least once annoyed by spectral overlap and crosstalks.

@mgpisfil is presenting a solution how to overcome crosstalks using #FLIM Phasor analysis

Q: how many fluorophores can you resolve?
A: how 5 color confocal sound? what about 5 color STED?

#FLIMSym

[Giorgia Susin @GiorgiaSusin]

Last day of practical sessions at #EMBLfluorescencelifetime course doing some multiplexing! Amazing 🤩 @EMBLEvents

[Mariano Gonzalez Pisfil @mgpisfil]

Doing some Biosensing experiments with @jalink_kees
@EMBLHeidelberg
@EMBLEvents
#EMBLFluorescenceLifetime

[Mariano Gonzalez Pisfil @mgpisfil]

Practical sessions start! Imaging some fluorescent colonies.
@EMBLHeidelberg
@EMBLEvents
#EMBLFluorescenceLifetime

[Mariano Gonzalez Pisfil @mgpisfil]

First talk by Dorus Gadella: introduction to the wonderful world of fluorescence lifetime.
@EMBLHeidelberg
@EMBLEvents
#EMBLFluorescenceLifetime

[Mariano Gonzalez Pisfil @mgpisfil]

Ready to kickoff!!! @EMBLHeidelberg
#EMBLFluorescenceLifetime

[Luis AJ Alvarez @L_AJ_Alvarez]

Today is going to be a good day 🙃. Everything is set to start the Fluorescence Imaging Beyond Intensities course at @EMBLEvents #EMBLFluorescenceLifetime excited to have professors @jalink_kees, @Marie_Erard75, @LizzHinde, @mgpisfil, Sergi Padilla, Dorus Gadella, Kai Johnsson

[Mariano Gonzalez Pisfil @mgpisfil]

TiM 2023 is over. It was a tiring and packed week, long days. But all in all an amazing experience. Thanks to the organizers, workshop providers, industry partners, and all the people who made this event a success!! See you in 2025!! Now holidays and sun! ☀️

#tim2023 @GerBI_GMB