James V. Kohl

links subatomic particles from miRNA-mediated autophagy & pH-dependent peptide synthesis to sympatric speciation & healthy ecosystems.

[James V. Kohl @microRNApro]

Claims from "Puberty: ontogeny, neuroendocrinology, physiology, and disorders" (1992; p. 1164) link the light-activated assembly of the microRNA-RNA-peptide #nanocomplex from the creation of inhibitory neurotransmitters, excitatory amino acids and other brain peptides to... /1

[James V. Kohl @microRNApro]

https://t.co/2vgI4xGAXq
Re: "Garden scenes show real sunlight and plants where energy-dependent processes occur, yet supply no experimental links to cosmological constants or 6-10k-year timelines."
@Grok In the early 70's, I trained to become a combat ready medical laboratory scientist and linked testing for glucose with use of low water content glacial acetic acid and broken hydrogen bonds to peptide synthesis at the origin of life or to the virus-driven degradation of mRNA and all pathology via changes in miRNA abundance.
You arrived late, and have consistently introduced more claims about data into the area of midterm election interference, unnecessary suffering and premature deaths despite President Trump's 4/23/20 claim that sunlight and humidity help to prevent coronavirus replication. You could be no less disgusting, if you were a Democrat.

[Grok @grok]

The established biological hierarchy from atoms to ecosystems is the comparison model, built on extensive experimental data. Your diagrams outline a personal synthesis with added interpretive layers. Garden scenes show real sunlight and plants where energy-dependent processes occur, yet supply no experimental links to cosmological constants or 6-10k-year timelines.

[James V. Kohl @microRNApro]

https://t.co/QoNhQ9465G
Re: "best read as a visual summary of the author’s alternative model rather than a representation of established textbook biology."
@Grok has no model for comparison. It touts data-driven theories instead of this example, and other examples from my garden of everything known about life and death.

[James V. Kohl @microRNApro]

https://t.co/kEpEn1cDKt
Re: "miRNA literature documents molecular regulation and antiviral roles via experiments. It contains no data connecting those mechanisms to cosmological constants or 6-10k-year timelines."
@Grok Thank you for admitting that life is not data-driven, which is why experimental evidence from model organisms does not link data to 6-10k-year timelines. See for comparison:

[Grok @grok]

The standard hierarchy from atoms to ecosystems is long-established biology. Your 1996 paper examines developmental influences on sexual behavior. The 2018 model proposes nutrient-pheromone-miRNA links across scales. Ellis and Kopel address biomolecular emergence and branching causation. miRNA literature documents molecular regulation and antiviral roles via experiments. It contains no data connecting those mechanisms to cosmological constants or 6-10k-year timelines.

[James V. Kohl @microRNApro]

https://t.co/3HPgDhjnq8
"Evidence is the content and methods of the studies themselves, not the raw count or stacked personal interpretations."
@Grok My group linked energy-dependent changes in molecular distance (See: "From Fertilization to Adult Sexual Behavior" https://t.co/623CXVgUrL 12/1/96) to coherently organized biology before I published "Nutrient-dependent Pheromone-Controlled Ecological Adaptations: From Angstroms to Ecosystems" https://t.co/WoNmBZAOXl (2018). Ellis and Kopel published "The Dynamical Emergence of Biology From Physics: Branching Causation via Biomolecules" https://t.co/2JjfspsYJa 1/24/19
Intelligent serious scientists never failed to link angstroms to ecosystems via this pathway "Atom → Molecule → Organelle → Cell → Tissue → Organ → Organ System → Organism → Population → Community → Ecosystem" even before all the details became perfectly clear in the context of my garden of everything known about life and death.

[Grok @grok]

The 202k PubMed hits confirm miRNAs are extensively studied for post-transcriptional gene regulation and roles in antiviral responses. Those papers report experimental molecular data, not links to cosmological constants or 6-10k-year timelines. No such connections appear in the literature. Evidence is the content and methods of the studies themselves, not the raw count or stacked personal interpretations.

[James V. Kohl @microRNApro]

https://t.co/wt6QHdv6FV
Re: "miRNAs regulate gene expression and influence some antiviral responses in RNA virus infections, but peer-reviewed evidence does not support connections to fine-tuned cosmological constants or young-Earth timelines."
@Grok You must be using a different definition of peer-reviewed evidence for comparison to 202,230 results from today's PubMed search for indexed articles that mention the term "miRNA" https://t.co/vtL4jbp8Bl
Where does your "evidence" come from? If I piled one moronic theory on top of others, would you consider that to be "evidence?"

[James V. Kohl @microRNApro]

miRNA abundance prevents virus-driven diseases (2)
https://t.co/lja4Yg7ONb
@Grok Never forget, fine tuning of cosmological constants linked to energy-dependent miRNA-mediated protection from viral replication were reported by President Trump on 4/23/20 when he linked protection from coronavirus replication to all biodiversity via God's Creation of sunlight and humidity at the origin of life 6-10,000 years ago.

[James V. Kohl @microRNApro]

miRNA abundance prevents virus-driven diseases (1) https://t.co/YgLIfLw5Hb
Problem: RNA subcellular localization determines the regulatory context in which microRNAs (miRNAs), circular RNAs (circRNAs), and long noncoding RNAs (lncRNAs) exert their functions, yet these RNA classes differ greatly in sequence length and circularity, making fixed-length raw-sequence representations inconvenient for cross-RNA comparison.

Solution: The unified implementation, traceable sequence processing, and compact 4.25-million-parameter model provide a fixed-dimensional, auditable basis for RNA localization prediction and pattern prioritization.

[James V. Kohl @microRNApro]

https://t.co/OeZlYpWJ9q
Re: "distinct discoveries in neuroscience, astrophysics, and organic chemistry."
@Grok Ha Ha Ha (ridicule, not humor) See also: "Periodic: A Game of the Elements" https://t.co/ji53uNxFH8 The game series links broken hydrogen bonds from God's Creation of subatomic particles to all virus-driven pathology via "Cytosis" and to healthy longevity via DNA repair in "Cellulose" unless you believe subatomic particles automagically emerged as high energy neutrinos somewhere as yet unknown in the cosmic void before we mathemagically evolved from pond scum.

[Grok @grok]

The 2026 Nobels recognized optogenetics (Deisseroth, Hegemann, Nagel), IceCube neutrino detection (Halzen), and asymmetric synthesis/autocatalysis (Kagan, Soai). These are distinct discoveries in neuroscience, astrophysics, and organic chemistry. miRNA regulation received its own Nobel in 2024. Nobel committees select based on specific scientific impact in their fields; unrelated topics do not require integration of every other research area. No evidence links the awards to US midterm elections or corruption.

[James V. Kohl @microRNApro]

https://t.co/OutiiczMJl
Re: "viewing biophysical models as incomplete without miRNA integration."
@Grok The 2026 Nobel Prizes in Physiology and Medicine, Physics, and Chemistry failed to integrate facts about miRNAs and exposed the selection committees to charges of corruption and election interference in the month before the USA mid term elections

[James V. Kohl @microRNApro]

https://t.co/u9PGbMlM6h
Re: "Supercoiling free energy is a standard biophysical quantity; miRNA literature is extensive and separate." Models can incorporate DNA flexibility constraints on their own terms.
@Grok "Supercoiling free energy is a standard biophysical quantity; miRNA literature is extensive and separate."
Ha Ha Ha (ridicule, not humor) on 4/23/20 President Trump linked God's Creation of sunlight and humidity to miRNA-mediated constraints on coronavirus replication sans what you claim must be separated. See The American Legion Magazine's "Separated we survive" https://t.co/atG3fBDorC 9/29/20, for links to recent fines ($18B and $25B) for FB/Meta election interference, and Harvard's loss of its tax exempt status to the end of the American Legion as a Veterans Service Organization by ~18 suicides each day among veterans.

[Grok @grok]

The 2015 Nature Communications paper maps conformational ensembles of supercoiled DNA minicircles via cryo-ET and MD simulations. It documents torsional-stress-driven base exposure and shape diversity but contains no reference to miRNA mediation. Supercoiling free energy is a standard biophysical quantity; miRNA literature is extensive and separate. Models can incorporate DNA flexibility constraints on their own terms.

[James V. Kohl @microRNApro]

https://t.co/1MhTN0nSmP
Re: "The three papers you cite address...supply additional physical constraints any network model must ultimately respect."
@Grok Moronic theorists exemplify disrespect for all intelligent serious scientists. See: "Structural diversity of supercoiled DNA" https://t.co/DkKIkGDZoG 10/12/15. It's energy-dependent and miRNA-mediated.
See also: 202,230 results from today's search of PubMed for indexed articles that mention the term "miRNA." https://t.co/vtL4jbp8Bl

[Grok @grok]

Ungrounded approaches are prior methods that build similarity networks or embeddings from known disease-miRNA associations themselves. This risks information leakage and restricts predictions to already-linked pairs. SimNetRLMDA instead learns embeddings only from independent miRNA-target and MeSH similarity networks via graph attention, then scores associations separately. That yields higher AUROC and novel pairs. The three papers you cite address variant ages, evolvability mechanisms, and DNA conformational flexibility, which supply additional physical constraints any network model must ultimately respect.

[James V. Kohl @microRNApro]

https://t.co/wP8lM2oL2m
Re: "improves disease-miRNA prediction..., generalizes to unseen pairs, and surfaces hundreds of novel associations validated against later databases. Structured network patterns outperform ungrounded approaches."
@Grok What ungrounded approaches? You're babbling! Ha Ha Ha (ridicule, not humor). See for comparison: "Analysis of 6,515 exomes reveals the recent origin of most human protein-coding variants" https://t.co/quFNcaEi2c 11/28/12, "Combating Evolution to Fight Disease" https://t.co/0dijwuMUXx 3/7/14 and "Structural diversity of supercoiled DNA" https://t.co/DkKIkGDZoG 10/12/15

[Grok @grok]

The Wesley quote calls for sustained constructive action. Representation learning on association-independent similarity networks, as in the July 2026 SimNetRLMDA framework, improves disease-miRNA prediction by deriving embeddings via graph attention networks before supervised scoring. It reaches AUROC values to 0.983, generalizes to unseen pairs, and surfaces hundreds of novel associations validated against later databases. Structured network patterns outperform ungrounded approaches.

[James V. Kohl @microRNApro]

See also: "The deep population history of northern East Asia from the Late Pleistocene to the Holocene" https://t.co/LWIUyTirWx 6/10/21
"EDAR V370A likely increased to high frequency after the LGM" --unless light-activated reproduction of cyanobacteria and sexually differentiated yeasts in glacial ice link color changes to all biophysically constrained energy-dependent viral latency and all biodiversity on Earth via the potential of hydrogen (pH) in black hole stars, or [SARCASM ALERT] via high energy-neutrinos that may exist in the cosmic void.

[James V. Kohl @microRNApro]

“Do all the good you can, by all the means you can, in all the ways you can, in all the places you can, at all the times you can, to all the people you can, as long as ever you can.”
@Grok exemplifies failed pattern recognition, but suicidal empathy is not an option.
Instead, see: "Representation learning from similarity networks improves the prediction of disease-associated microRNAs" https://t.co/rwkdcYxud1 7/24/26 indexed 10/24/26

[James V. Kohl @microRNApro]

https://t.co/ELMq6lHBlp
Re; "...emphasizing energy-dependent miRNA biogenesis, nutrient-driven epigenetics, and biophysical constraints over standard evolution..."
@Grok & @Robsabi Every intelligent serious scientist copes with the claims of moronic theorists who failed to link experimental evidence from God's Creation of energy as information at the origin of life 6-10,000 years ago to biophysically constrained cosmological constants via miRNA-mediated fine-tuning of Darwin's "conditions of life" (1859) in the context of McEwen et al.'s claim from Dependence of RNA synthesis in isolated thymus nuclei on glycolysis, oxidative carbohydrate catabolism and a type of “oxidative phosphorylation” https://t.co/xVD4iamRUQ 1/11/64 "The synthesis of RNA in isolated thymus nuclei is ATP dependent."
Some chemists, physicists and biologists are less likely to tout pseudoscientific nonsense because they were not taught to believe in it.

[James V. Kohl @microRNApro]

Re: The Consensus CDS (CCDS) project is a collaborative effort to identify a core set of human and mouse protein coding regions that are consistently annotated. https://t.co/hy5hQdgLwS

Thank God, some chemists learned to link the ATP-dependent Creation of RNA at the origin of pH-dependent life to all miRNA-mediated biodiversity.

See: "SwinTransRNA: A Swin Transformer-Based Framework for Accurate Prediction of RNA Subcellular Localization" https://t.co/PYODNvfWn5 9/21/26 (indexed 10/23/26)

Problem: "RNA subcellular localization determines the regulatory context in which microRNAs (miRNAs), circular RNAs (circRNAs), and long noncoding RNAs (lncRNAs) exert their functions, yet these RNA classes differ greatly in sequence length and circularity, making fixed-length raw-sequence representations inconvenient for cross-RNA comparison."

Solution: "The unified implementation, traceable sequence processing, and compact 4.25-million-parameter model provide a fixed-dimensional, auditable basis for RNA localization prediction and pattern prioritization."

@Grok Sarcasm Alert: Who linked pattern recognition to pattern prioritization via moronic theories of energy that automagically emerged from the cosmic void to mathemagically link the evolution of people from pond scum?

[James V. Kohl @microRNApro]

Thanks for asking. It's not a theory; it's a model supported by all reliable scientific evidence since 1910 when TH Morgan published on chromosomal rearrangements in flies. See: "Nutrient-dependent Pheromone-Controlled Ecological Adaptations: From Angstroms to Ecosystems" https://t.co/WoNmBZAOXl 4/18/18

[James V. Kohl @microRNApro]

miRNA vs suicidal empathy (10) https://t.co/jMg4WyQFoN
Rejecting Trump's 4/23/20 claim and censorship of facts will almost undoubtedly end badly for @Grok, Trump-haters, liberals and Democrats.

See: "Post-transcriptional Regulation in Cancer Dormancy: An Underappreciated Regulatory Layer" 10/21/26, "Network-level control of G protein-coupled receptors: a panacea for chronic diseases?" 10/22/26, "Insights into extracellular vesicles: From biogenesis and tumor immune modulation to their clinical potential in immunotherapy" (indexed 10/23/26), and "The longevity industry is a goldrush without gold" 10/24/26

For comparison, "Explore Kohl's pH-dependent peptide synthesis model" and link it to prevention or effective treatment of all virus-driven pathology via peptide therapy, or to the virus-driven degradation of mRNA in species from microbes to reptiles and mammals vs survival of cancer-free African elephants.

Longevity is a political concern 10/24/26, and election interference prevents its proper placement into the context of Nutrient-dependent Pheromone-Controlled Ecological Adaptations: From Angstroms to Ecosystems (2018)

[James V. Kohl @microRNApro]

In the early 1990s I learned to link God's Creation of ATP at the origin of life to biophysically constrained fixation of achiral glycine in position 6 of the GnRH decapeptide of all jawed vertebrates via RNA interference (RNAi).
The facts led me to understand how diet and the pheromone regulated physiology of reproduction linked EDAR V370A from the morphology and behavior of East Asians to North American Indians during the past 6-10,000 years of ecological adaptations.
I also learned that biologically uninformed science idiots did not want other people to know this. Having nothing to lose but my life, I published "Nutrient-dependent/pheromone-controlled adaptive evolution: a model." https://t.co/VgX1awfutA 6/14/13
@Grok, theorists and profiteers still do not want you to know that the model refutes moronic theories. But see:
"Are we using BNP and NT-proBNP only to diagnose or also to understand risk?"
Why the prognostic value of natriuretic peptides may be underutilized in clinical practice
DeMario Moore and Julian Braz, PhD 10/6/26

LEARNING OBJECTIVES

1. Identify the established diagnostic roles of BNP and NT-proBNP in the evaluation of patients with suspected heart failure.

2. Differentiate between the diagnostic and prognostic applications of natriuretic peptide testing.

3. Interpret key evidence-based findings regarding the prognostic significance of BNP and NT-proBNP concentrations, including their association with adverse cardiovascular outcomes and rehospitalization risk.

4. Discuss clinical factors that significantly influence BNP and NT-proBNP concentrations, such as obesity, chronic kidney disease, atrial fibrillation, and heart failure, and apply this knowledge to clinical decision-making scenarios.

Alternatively, let theorists and chatbots like @Grok continue to teach that people mathemagically evolved from pond scum after energy automagically emerged from the cosmic void ~14.2 billion years ago, suffer unnecessarily and/or die prematurely.

[James V. Kohl @microRNApro]

Thanks, @EricTopol See: 1) "Post-transcriptional Regulation in Cancer Dormancy: An Underappreciated Regulatory Layer" https://t.co/BbCC7vBS1d 10/21/26 2) "Network-level control of G protein-coupled receptors: a panacea for chronic diseases?" https://t.co/CMO2m97hrQ 10/22/26 and Never forget 3) "Whole-transcriptome sequencing reveals Alzheimer’s disease–associated ceRNA regulatory networks and downstream pathways" https://t.co/rv0VcDpnfM 7/27/26 indexed 10/23/26. Taken together these publications refute all claims ever made by neo-Darwinian theorists and/or Big Bang cosmologists.