Melody Lindsay

research scientist thinking about extreme environments, activity of microbial cells, and the potential for life
🌌🌎🪨💦✨️🦠🔬🤷‍♀️

[Melody Lindsay @mrlindsa]

omg sigma aldrich 'shipping on time, very considerate'

[Sigma-Aldrich @SigmaAldrich]

You see our bottle? We keep it very classy, very mindful, very demure.

[Melody Lindsay @mrlindsa]

So very excited and thrilled that this is funded!! It's always amazing and a real pleasure to do my job, but it's somehow more amazing when you get to work with your bestie on ideas that have spawned out of a cross-discipline, long-term friendship (15 years since DO!!🤣)

[Melody Lindsay @mrlindsa]

@spearmicrobe @minesqbe @coschoolofmines in the nanoBoyds? 😂

[Bigelow Laboratory @BigelowLab]

CTD Sunrise: Julia Brown and Tim D’Angelo are aboard @SchmidtOcean’s Falkor (too) on a cruise in collaboration with Maria Pachiadaki from @WHOI to sample microbes that live in oxygen minimum zones. Here Tim is working with Annabelle Adams-Beyea, a former Bigelow Laboratory REU!

[Melody Lindsay @mrlindsa]

@cejasper @JRSO_IODP stunning!!!

[Claire Jasper @cejasper]

Happy Earth Month from *Reykjanes Ridge* in the North Atlantic 🥹@JRSO_IODP

[Melody Lindsay @mrlindsa]

It's been amazing to be a part of the great team working on this (peep the author list 👀), and I look forward to what else we can uncover with single cell activity questions targeted at interesting but challenging environments that are low in biomass or activity.

[Melody Lindsay @mrlindsa]

- and single-cell genomic sequencing (thanks to Ramunas Stepanauskas at the Single Cell Genomics Center). Leaders of this genomes-to-phenome project along with Duane Moser at the Desert Research Institute, and my amazing mentors/postdoc advisors @DeepMicrobe and David Emerson!

[Melody Lindsay @mrlindsa]

This method involved calibration of a fluorescence-based activity proxy (using the RedoxSensor(TM) Green fluorophore), flow cytometric measurements and sorting (thanks to @njpoulton at the Center for Aquatic Cytometry!), -

[Melody Lindsay @mrlindsa]

We demonstrate that there is variability in single-cell respiration rates within cells of the same lineage (≥95 ANI) and that the charismatic micro-fauna Ca. Desulforudis audaxviator is indeed responsible for a large portion of active elemental cycling.

[Melody Lindsay @mrlindsa]

With this work, we are able to resolve single-cell rates of sulfate reduction in the deep subsurface from measurements made at the level of a single cell, not previously possible. This provides insights into deep life persisting in isolated, rock-hosted environments! 🪨🦠

[Melody Lindsay @mrlindsa]

New paper!!! A thread 😸 - I am happy to share our new publication on "species-resolved, single-cell respiration rates" that we were able to measure in a deep continental subsurface ecosystem!
https://t.co/0BW4MXYtYh

[lettuce @the_lettuce_cat]

Partial eclipse as seen through a cheezit

[Melody Lindsay @mrlindsa]

@DeepMicrobe hehe we are hanging in there! hope you didn't lose power for too long (as I assume pretty much almost everyone in Maine lost power for at least some time...)

[Melody Lindsay @mrlindsa]

@DeepMicrobe @BigelowLab Thank you Beth! So excited that our work is out!!! :)

[Melody Lindsay @mrlindsa]

oh look, it me

[Dr Meming @Dr_Meming]

Me: ok I’ll just repeat that simple experiment and I’ll be one step closer to finishing that paper. It worked last time so should work this time

The experiment:

[Bigelow Laboratory @BigelowLab]

OSM, OMG! There’s a full gaggle of Bigelowians at #OSM24. If you’re here too, visit us at booth #241 any time or bring yourselves, your colleagues, and warm beignets (if you’ve got them) to Rivergate Terrace, second floor tonight (Thurs) at 6:30 p.m. for a little gathering/Q&A.

[Chris Lindsay @chris_j_lindsay]

New paper out today. 👀 We use asteroseismology to model 62 subgiant stars observed by Kepler and TESS, and study the fossil signatures left behind by their main-sequence convective cores🌟. Read about it here https://t.co/0ac1yddYvS.

[Melody Lindsay @mrlindsa]

@TheJR stromatolites!!!!! 💚💚

[Bigelow Laboratory @BigelowLab]

We’re currently taking applications for our 2024 Bioinformatics of Microbial Single Cells Workshop here in East Boothbay April 2-8. Applications are due by January 5, and registration is limited, so inquire today. For more information visit: https://t.co/pvC0FNk6bH