I wish students knew how much more work goes into teaching online courses compared to in-person courses. Rather than just being lecturers, we are now also web designers and video editors and content creators. This is exhausting.
16/
A soft yes.
A hard no.
Insistence of a spouse.
A hero publicly taking the vaccine.
A belief that something freely offered to more than just the rich is suspect.
And a lived experience that supports that idea.
These were their whys.
Theirs.
Yeah.
#BlackWhysMatter
@GilleighD Yes, as an alt. to GST. We’ve tried just GST-tag in the past with both bacterial and insect cells, with not much success. So I’m wanting to compare Sumostar in insect cells to a dual-tag system in insect cells. Only have found two pubs, but don’t know anyone else who’s used it
Does anyone have experience using SUMOStar expression systems in insect cells and is it worth the cost? Need help comparing it to a typical GST-protein-His dual tag system before I convince my advisor that it’s a good investment 😅
@klausenhauser I’ve tried bacterial expression systems for about a year, but my protein is just not very functional or soluble. It is a fly protein, so my hope is using Sf9 cells to express will help. I would like to clarify I want to compare SumoStar to using GST-His in Sf9 cells
yo if people aren’t taking their vaccine lemme have one i want to be able to workout outdoors again without the crippling fear that i might get my mom sick.
My lab is looking for new grad students. We study multi-sensory-motor integration in drosophila and manduca. We take a neuroethological approach:behavior physiological, and anatomy (including connectomics) approaches. Interested? Contact me. Friends, please retweet 🙏