Last Friday, we wrapped up #EMBLFluorescenceLifetime 🔬✨
Thank you to all the participants, trainers, speakers, and organisers who made this course such a success! We hope you enjoyed it and feel confident to put your new skills into action! 🤝
See you next time!
🔵 Scientific organisers:
Luis Alvarez, Leica Microsystems, Germany
Manuel Gunkel, EMBL Heidelberg, Germany
Birgit Koch, Max Planck Institute for Medical Research, Germany
Andrea Müller, Leica Microsystems, Germany
🔵 Course organiser:
Karo Wolkersdorfer, EMBL Heidelberg, Germany
During the 5 day #EMBLFluorescenceLifetime course, participants get hands-on training every afternoon on our state-of the art microscopes 🔬
Practicals include Halo-tags & FLIM on STELLARIS inverted DIVE, Multiplexing with FPs and FLIM on STELLARIS 8 STED, as well as FLIM analysis including ML and AI on STELLARIS Cryo 🤩
Demonstrating super-resolution imaging and single-molecule detection with flippers in the latest paper of the Fürstenberg lab in collaboration with the Matile group @afuerstenberg@matile_group
https://t.co/4uftw2IuRX
The whole Flipper-TR family of mechanosensitive probe goes #SMLM & PAINT thanks to Jimmy Maillard, Ewa Grassin, @pricelesska and colleagues in a joint effort from @matile_group and Alex Fürstenberg labs in @sciences_UNIGE.
Congrats!
https://t.co/qNTuz596Cz
Check out our new paper🎉
https://t.co/H1DAosQkvc
Mechanosensitive Flipper-TR from @matile_group can be used for #SMLM in model membranes:
✨Single-molecule tracking of Flipper-TR in lipid bilayers with varying fluidity
✨Super-resolution imaging of both Lo and Ld GUVs
📢#PaperAlert
In our new paper we introduce GFP-inspired thiol-reactive fluorophores 🌟🌈
Their spectra sense ligand-induced structural changes in proteins 🧪🔬 #GPCR
Our dye outperforms bimane, Cy-dyes & TMR💪
https://t.co/xT5vg2zGwV
@iScience_CP
Warm thanks to all authors!
These are PALM movies of monomeric and dimeric membrane proteins labeled with mEos3.2 in fixed HEK293 cells ✨✨✨
https://t.co/Hp0iya7yqh
https://t.co/lAVmMOe8hy
https://t.co/XPhga7Vb77
https://t.co/whV8kPphfo
anyway, having mentioned data on Zenodo...
If you are looking for raw #SMLM#PALM microscopy data to train localization or clustering algorithms, to analyze fluorescent proteins blinking, or for any other purpose, you might be interested in this! ⬇️
We celebrate first first-author paper by @pricelesska🥳
Using super-resolution #PALM🔬, we showed that ChR2, protein used to control neuronal activity with light in lab and clinics, forms stable (WT) or transient (mutant) dimers in human cell membranes
https://t.co/300ZzYthnu
Nevertheless, there's some good news – our joint work with Dominik Wöll and Alexandre Fürstenberg is finally out. It demonstrates how we can utilize FL-SMLM for environmental sensing, specifically determining local water content in polymer gels.
https://t.co/c5Uk2pioCV