Ekaterina Bestsennaia

PhD student at @sciences_UNIGE
illuminating little mysteries of life 🌈🔬🧫

[EMBL Events @EMBLEvents]

Last Friday, we wrapped up #EMBLFluorescenceLifetime 🔬✨

Thank you to all the participants, trainers, speakers, and organisers who made this course such a success! We hope you enjoyed it and feel confident to put your new skills into action! 🤝

See you next time!

🔵 Scientific organisers:
Luis Alvarez, Leica Microsystems, Germany
Manuel Gunkel, EMBL Heidelberg, Germany
Birgit Koch, Max Planck Institute for Medical Research, Germany
Andrea Müller, Leica Microsystems, Germany

🔵 Course organiser:
Karo Wolkersdorfer, EMBL Heidelberg, Germany

[EMBL Events @EMBLEvents]

During the 5 day #EMBLFluorescenceLifetime course, participants get hands-on training every afternoon on our state-of the art microscopes 🔬

Practicals include Halo-tags & FLIM on STELLARIS inverted DIVE, Multiplexing with FPs and FLIM on STELLARIS 8 STED, as well as FLIM analysis including ML and AI on STELLARIS Cryo 🤩

[Ekaterina Bestsennaia @pricelesska]

See you there
https://t.co/5AGLW0qxq8

[Ivan Maslov @IvanMasl0v]

@ScienceLinX 7 days before the #microscopiedag (Nov 9th) in Groningen & Drenthe! https://t.co/Y9ZOKTbXHl

Soap bubbles under the microscope 🔬🫧🫧🌈

🧵34/n @ScienceLinx #Microscopy

[Ekaterina Bestsennaia @pricelesska]

@alcien_calie Congratulations! 🎉

[Ekaterina Bestsennaia @pricelesska]

My happy chance to present a poster at @SMLM_Symposium (and enjoy late summer in Portugal 🌊)

#SMLMS2024

[The Journal of Physical Chemistry @JPhysChem]

Demonstrating super-resolution imaging and single-molecule detection with flippers in the latest paper of the Fürstenberg lab in collaboration with the Matile group @afuerstenberg @matile_group
https://t.co/4uftw2IuRX

[Spirochrome @spirochrome]

The whole Flipper-TR family of mechanosensitive probe goes #SMLM & PAINT thanks to Jimmy Maillard, Ewa Grassin, @pricelesska and colleagues in a joint effort from @matile_group and Alex Fürstenberg labs in @sciences_UNIGE.

Congrats!

https://t.co/qNTuz596Cz

[Ekaterina Bestsennaia @pricelesska]

Check out our new paper🎉
https://t.co/H1DAosQkvc

Mechanosensitive Flipper-TR from @matile_group can be used for #SMLM in model membranes:
✨Single-molecule tracking of Flipper-TR in lipid bilayers with varying fluidity
✨Super-resolution imaging of both Lo and Ld GUVs

[Ivan Maslov @IvanMasl0v]

📢#PaperAlert

In our new paper we introduce GFP-inspired thiol-reactive fluorophores 🌟🌈

Their spectra sense ligand-induced structural changes in proteins 🧪🔬 #GPCR

Our dye outperforms bimane, Cy-dyes & TMR💪

https://t.co/xT5vg2zGwV
@iScience_CP

Warm thanks to all authors!

[Ekaterina Bestsennaia @pricelesska]

See you at @Sfb1348 meeting on Molecular Mechanisms of Membrane Organization 🙌

[Ekaterina Bestsennaia @pricelesska]

@elleelize what a great result, congrats! 🎊

[Ekaterina Bestsennaia @pricelesska]

very relatable conclusions 🥲
from a great talk on the fundamental properties of fluorescent proteins (FPs) by @BourgeoisDom2
#fluorescenceleshouches

[Ekaterina Bestsennaia @pricelesska]

🌈 molecular gastronomy by @NathanShaner at the École de physique des Houches #fluorescenceleshouches

[Ekaterina Bestsennaia @pricelesska]

These are PALM movies of monomeric and dimeric membrane proteins labeled with mEos3.2 in fixed HEK293 cells ✨✨✨

https://t.co/Hp0iya7yqh
https://t.co/lAVmMOe8hy
https://t.co/XPhga7Vb77
https://t.co/whV8kPphfo

[Ekaterina Bestsennaia @pricelesska]

turned out my brain replaces 'date' with 'data', and this explains a lot

[Anastasia Atamas @BeatlNasty]

Prof deformation
- Have you ever used ZenDate?
- I just downloaded data from the article on Zenodo
- Lol, ZenDate is a dating app 😅

[Ekaterina Bestsennaia @pricelesska]

anyway, having mentioned data on Zenodo...

If you are looking for raw #SMLM #PALM microscopy data to train localization or clustering algorithms, to analyze fluorescent proteins blinking, or for any other purpose, you might be interested in this! ⬇️

[Ekaterina Bestsennaia @pricelesska]

One small step for the rest, one giant leap for me! Happy and thankful to all involved 🥹

[Ivan Maslov @IvanMasl0v]

We celebrate first first-author paper by @pricelesska🥳

Using super-resolution #PALM🔬, we showed that ChR2, protein used to control neuronal activity with light in lab and clinics, forms stable (WT) or transient (mutant) dimers in human cell membranes

https://t.co/300ZzYthnu

[Angewandte Chemie @angew_chem]

Channelrhodopsin-2 Oligomerization in Cell Membrane Revealed by Photo-Activated Localization Microscopy (Valentin Borshchevskiy and co-workers) @pricelesska @IvanMasl0v @alekseyka1993 @Aynya5 @dzabelskii @heilemannlab @BoshchevskiyV #openaccess https://t.co/XCvSJp5lQp

[AlexNevs @OleksiiNevskyi]

Nevertheless, there's some good news – our joint work with Dominik Wöll and Alexandre Fürstenberg is finally out. It demonstrates how we can utilize FL-SMLM for environmental sensing, specifically determining local water content in polymer gels.
https://t.co/c5Uk2pioCV