ProImmune

Mastering Immunity: helping you to understand and manage immune responses, with our unique products and services

[ProImmune @proimmune]

Spectacular cell structure #FluorescenceFriday, with
an #animalfree Ankyron™ against Calreticulin to specifically label the endoplasmic reticulum (red) in HUVEC, counterstaining actin cytoskeleton (green) and nuclei (blue) @Discover_Echo

#bioscience #immunology #3Rs
@AFR_UK

[ProImmune @proimmune]

This #FluorescenceFriday we use our Ankyron CJ30442 (cyan) to identify the lymphatic marker LYVE1 in skin, showing the extensive lymphatic network through to the individual oak-leaf-shaped endothelial cell on the Mica #microscope @LeicaMicro. Learn more at https://t.co/JojkqG26d0

[ProImmune @proimmune]

This #FluorescenceFriday we are shining a light on lymphatics in skin using Ankyron® clone DE40213, which binds Podoplanin (cyan). Image captured with our @Discover_Echo #microscope. Learn more about our recombinant antibody-replacements at https://t.co/JojkqG2E2y

[ProImmune @proimmune]

This #FluorescenceFriday we used our recombinant target binding reagent Ankyron® clone CE30111 (green) to detect Perlecan in the extracellular matrix deposited by HUVECs, with nuclei counterstained using DAPI (blue). Image captured using our brilliant @Discover_Echo #microscope.

[ProImmune @proimmune]

This #FluorescenceFriday we’re exploring microtubules using Ankyron® clone AP40945, which binds TBCA (red), with DAPI-stained nuclei (blue). Shown in HeLa cells. Image captured with our lovely @Discover_Echo #microscope. Learn more: https://t.co/JojkqG26d0

[ProImmune @proimmune]

If #FlowcytometryFriday is more your thing, then Ankyron™ AC40302 works well in this application, shown in HeLa (red histogram, with negative control of secondary fluorophore only in grey). Ankyrons™ are #animalfree recombinant next-generation binding and detection reagents.

[ProImmune @proimmune]

Fascinating insights this #FluorescenceFriday, into complement delay-accelerating factor (DAF, CD55), identified by our Ankyron™ AC40301 (red) in HeLa cells (nuclei, blue) on our lovely @Discover_Echo #microscope. #animalfree #immunology #cells

[ProImmune @proimmune]

Exquisite filamentous patterns for #FluorescenceFriday thanks to Ankyron™ ANK00782_7_AU41488 specific to CD29 cell surface receptor β1 integrin (shown in red in a dendritic cell, with nucleus counterstained with DAPI blue).

@Discover_Echo

#bioscience #immunology #cells

[ProImmune @proimmune]

#MicroscopyMonday! IHC with an insulin-specific #animalfree Ankyron™ AB40258 (brown) clearly identifying β-cells in islets of Langerhans of human pancreas (nuclei in blue) using #Leica autostainer and @Discover_Echo #microscope in colour camera, upright configuration.

[ProImmune @proimmune]

This #FluorescenceFriday we use an Ankyron™ to specifically label the Golgi apparatus marker RCAS1 (red), counterstaining actin cytoskeleton (green) and nucleus (blue) on @Discover_Echo microscope. Look out for our other organelle-specific #animalfree Ankyrons™

[ProImmune @proimmune]

We were happy to see that this Ankyron also works well in #FlowCytometry, with HUVEC stained with V5-tagged Ankyron and anti-V5 APC (red). Negative control, anti-V5 APC only (grey).

[ProImmune @proimmune]

Shining a light on Endoglin (aka CD105) for #FluorescenceFriday, shown in red using #animalfree Ankyron™ ANK00708_1_AY40419 (nuclei blue), highly expressed in vascular endothelial cells like these HUVEC & has a crucial role in angiogenesis, & TGFβ signaling.

@Discover_Echo

[ProImmune @proimmune]

This dendritic cell is ready for #FluorescenceFriday! Here, we used our interferon-α8-specific #animalfree Ankyron™ ANK01178_5_AW40550 (red) and nuclei counterstained with DAPI (blue) on @Discover_Echo #microscope
Find out more:
https://t.co/Y3enyiCfrk
#cytokine
@AFR_UK

[ProImmune @proimmune]

#FluorescenceFriday insight into Lupus La protein, highly distinctive cytoplasmic staining (red) revealed by our #animalfree Ankyron™ - nuclei counterstained with DAPI (blue).
@Discover_Echo

#bioscience #immunology #animalfreeresearch #cytoplasm
@AFR_UK

[ProImmune @proimmune]

Delighted that we contributed to exciting work from Zuben Sauna’s lab (FDA) using Ankyrons™ specific to 3 #COVID19 spike protein variants, which neutralized all 3 in a pseudovirus-based neutralization assay.

https://t.co/xhgB2QTuV3
#BLI #immunology #animalfree

[ProImmune @proimmune]

It's #FluorescenceFriday! Here's a lovely clear image using #animalfree Ankyron™ specific to cell cycle protein Cyclin A1 (red), with F-actin counterstained by phalloidin (green) @Discover_Echo

Find out more https://t.co/Y3enyiCNgS

#immunology #3Rs #cellcycle
@AFR_UK

[ProImmune @proimmune]

Our very own Emilee Knowlton, Ph.D., Senior Immunology Sales Specialist, will be speaking at this event about "An Integrated Approach to Managing Immunogenicity Risk and Optimum Protein Design".

[ProImmune @proimmune]

We're sponsoring #festofbiologics where science meets strategy!

Join us on April 23-24 in San Diego. From cutting-edge research to advancements in AI-driven drug discovery, this is where breakthroughs happen.

[ProImmune @proimmune]

And here's the same #animalfree Ankyron™ specific to β-actin (red), lighting up the cytoskeleton that we highlighted for #FluorescenceFriday last week:

[ProImmune @proimmune]

Is #WesternBlotWednesday a thing?! Why not celebrate those perfect western blots? Here's our #animalfree Ankyron™ specifically binding human β-actin (37.3 kDa), both recombinant protein and in cell line lysates. Perfect loading control for westerns!

#3Rs #westernblot
@AFR_UK

[ProImmune @proimmune]

Fab fine filaments of the cytoskeleton for #FluorescenceFriday lit up by our #animalfree Ankyron™ to β-actin (red), with nuclei counterstained with DAPI (blue) @Discover_Echo

A good alternative counterstain to phalloidin!

#bioscience #immunology #3Rs
@AFR_UK