@mika_hyshi@SerendipityWrk Thanks very much for your questions. In this work, the mold of double-layered microchannel was fabricated by the two-step photolithography technology. And the microchannel used was the PDMS replica through the silicone mold and bonded with a glass slide.
@JGaladePablo@SerendipityWrk Thanks. As you see, the flow rate of each plot is the total flow rate including the sample and sheath flow. And the figure on X-axis refers to the flow rate ratio between sheath flow and sample flow.