🌾 29 genes edited simultaneously in a single rice plant. No Cas12a. Just TREX2-SpCas9 + optimized tRNA arrays.
New in Plant Communications: TREX2 fusion boosts SpCas9 at tough loci (0% → 9.38%), and 38 rice tRNAs were screened to find 13 high-efficiency elements — two hit 100%.
✅ 83% of T0 plants: 19–27 OsCPK genes edited simultaneously
✅ Two plants: all 29 target genes knocked out
✅ Works in N. benthamiana too
Zhou labs, CAAS/ZJU | Plant Communications
https://t.co/L2GjuFbFlO
#CRISPR #MultiplexEditing #Rice #PlantBiology #SpCas9
🚨Plant Biotech EXCLUDED from ARS 2025!🚨Despite its key role in food security, climate resilience & crop innovation, ICAR has sidelined Plant Biotech from 8 years This neglect is unacceptable! We urge authorities to take action
#ReviseARS2025withAgriBiotech#ARSneedsAgriBiotech