Richard Held

Neuroscientist. Molecular ultrastructure of synapses with #cryoET and other fun tools.

Over on bsky now

[Richard Held @rgsheld]

My latest bit of work is up as a pre-print. We made an AMPA receptor GluA2 knock-in mouse in order to label and localize endogenous AMPARs at the synapse using cryoET. More details below and on biorxiv here:
https://t.co/OSo6Mzy1qA

[Richard Held @rgsheld]

@james_krieger Working on it and will update when they're up

[Richard Held @rgsheld]

My latest bit of work is up as a pre-print. We made an AMPA receptor GluA2 knock-in mouse in order to label and localize endogenous AMPARs at the synapse using cryoET. More details below and on biorxiv here:
https://t.co/OSo6Mzy1qA

[Richard Held @rgsheld]

A brief lull in work intensity to allow me to dwell in anxiety over the election.

[Richard Held @rgsheld]

@RaduAricescu Thank you! Pretty excited about it

[Richard Held @rgsheld]

@igreger1 Thanks!

[Richard Held @rgsheld]

Main change is a better movie 😬

[Richard Held @rgsheld]

Updated version of the preprint here:

https://t.co/BuEUyx5REi

[Richard Held @rgsheld]

My latest bit of work is up as a pre-print. We made an AMPA receptor GluA2 knock-in mouse in order to label and localize endogenous AMPARs at the synapse using cryoET. More details below and on biorxiv here:
https://t.co/OSo6Mzy1qA

[Richard Held @rgsheld]

@vikasnavaratna Thanks! Still lots to do

[Richard Held @rgsheld]

@jjanetzko Thanks!

[Richard Held @rgsheld]

@christlet

[Richard Held @rgsheld]

I'm excited to move forward with this work. After staring at cartoon schematics of the synapse for over a decade, this work convinced me that we can use that built-up knowledge to make real molecular maps and even atomic models of something as complex as the synapse.

[Richard Held @rgsheld]

We can tie it all together and look at how this maps back to our tomograms. AMPARs in complex with PSD scaffolds form dense clusters, with free receptors decorating the periphery, and docked vesicles around the cluster perimeter.