Excited to share my final PhD work from the Al-Hashimi lab at @Columbia titled “Thermodynamic prediction of RNA cellular activity from sequence via conformational ensembles” is now out in Cell @CellCellPress:
https://t.co/rnPhtbJqRU
Working with my amazing lab mate @GengAinan, we used NMR combined with binding & cellular measurements to show that conformational ensembles provide the energetic link to go from RNA sequence to cellular activity using existing RNA secondary structure prediction tools.
Kinetic Dissection of Proton-Coupled Conformational Transitions in Nucleic Acids by Integrating pH-Dependent NMR and Chemical Modifications | Journal of the American Chemical Society @Columbia https://t.co/cG5nsd1yKS
Cool new work from the lab, led by @GengAinan looking at the structure of an “invisible” RNA conformational state enriched in mismatches! https://t.co/GWKJltAWrn
Kinetic Resolution of the Atomic 3D Structures Formed by Ground and Excited Conformational States in an RNA Dynamic Ensemble | Journal of the American Chemical Society @Columbia#Structure#RNA#Dynamics#ExcitedState#Conformation https://t.co/fqbbEByATZ
1/ Negative is also positive! Our new study shows that mismatches in RNA:DNA hybrids can adopt a negative charge and have a substantial effect on fidelity of biochemical processes, such as transcription, translation and CRISPR/Cas9 off-target gene editing!
https://t.co/twDD0w0Uon
Cool new PNAS perspective from my advisor Hashim Al-Hashimi on describing biological systems as computational machines, titled: Turing, von Neumann, and the computational architecture of biological machines | PNAS https://t.co/FYVhPNUUrw
It's been a week folks! The project that defined my PhD has been published today in @Nature: https://t.co/AAZEJ8tEyW. Congrats to everyone involved in this work and a huge thank you to Hashim and Dan for their mentorship!! Also, my name is not a typo ! 1/2