Tsegaselassie Workalemahu

[Tsegaselassie Workalemahu @TWorkalemahu]

@alanephro @WorkalemahuT @HyperAHA @frank_spradley A16/2. The methylation patterns we found could serve as sentinels for future risk of cardiovascular disease and contribute to a generalized application of personalized medicine. #HYPHIP @HyperAHA

[Tsegaselassie Workalemahu @TWorkalemahu]

@alanephro @WorkalemahuT @HyperAHA @frank_spradley A16/1. Our objective was to understand the mechanisms of developmental origins of cardiometabolic factors using DNA methylation patterns in the placenta. #HYPHIP @HyperAHA

[Tsegaselassie Workalemahu @TWorkalemahu]

@NakagawaPablo A15/2. The sub-group sample sizes are too small in our data to detect associations by PreE or gestational hypertension. #HYPHIP @HyperAHA

[Tsegaselassie Workalemahu @TWorkalemahu]

@NakagawaPablo A15/1. We believe the normal range of blood pressure and placentas being collected at term will hinder potential associations with other PreE related markers. #HYPHIP @HyperAHA

[Tsegaselassie Workalemahu @TWorkalemahu]

@NakagawaPablo A13. Yes, this is an important follow up study we are able to carry out using the available placental histopathological biopsies. #HYPHIP @HyperAHA

[Tsegaselassie Workalemahu @TWorkalemahu]

@georgehret @WorkalemahuT @HyperAHA A13/2. Each of these tests were statistically corrected for multiple testing, providing a robust screening approach. #HYPHIP @HyperAHA

[Tsegaselassie Workalemahu @TWorkalemahu]

@georgehret @WorkalemahuT @HyperAHA A13/1. Yes, we attempted to test the associations of the methylation of each CpG, across the genome, with blood pressure of each woman. #HYPHIP @HyperAHA

[Tsegaselassie Workalemahu @TWorkalemahu]

@frank_spradley @HyperAHA A12/2. We, however, compared our methylation analysis with other datasets and showed CpG coordinately methylated with blood pressure and that are shared with preeclampsia in the placental DNA. #HYPHIP @HyperAHA

[Tsegaselassie Workalemahu @TWorkalemahu]

@frank_spradley @HyperAHA A12/1. We also did not identify associations between normal variation of blood pressure and placental methylation alterations in sFLT-1 gene. Future studies should include preeclamptic women to unravel methylation patterns of sFLT-1 in placenta. #HYPHIP @HyperAHA

[Tsegaselassie Workalemahu @TWorkalemahu]

@frank_spradley @HyperAHA @alanephro A. We did not identify association between normal variation of blood pressure and placental methylation alterations in PGI2 or thromboxane genes. #HYPHIP @HyperAHA

[Tsegaselassie Workalemahu @TWorkalemahu]

@guto_montezano @WorkalemahuT @frank_spradley @HyperAHA Q10. We sought to investigate whether maternal blood pressure during pregnancy (as an exposure) influences DNA methylation in the placenta at term. #HYPHIP @HyperAHA

[Tsegaselassie Workalemahu @TWorkalemahu]

@mari_br_montrea Q7. The heterogeniety in the cell composition of the placenta is a limitation in our study. Single-cell sequencing strategies are suggested, however, we tried to account for the heterogeneity in our study. #HYPHIP @HyperAHA

[Tsegaselassie Workalemahu @TWorkalemahu]

@Carmendemigue12 @WorkalemahuT @HyperAHA A6. We were not able to follow the offspring to address the important question. We compared our methylation analysis with other datasets that showed methylated CpGs in offspring blood #HYPHIP @HyperAHA

[Tsegaselassie Workalemahu @TWorkalemahu]

@frank_spradley @HyperAHA A6/2. Thus, placental DNA methylation assessment pre-term and during pregnancy may be a good strategy to unravel the complex temporal relationships. #HYPHIP @HyperAHA

[Tsegaselassie Workalemahu @TWorkalemahu]

@frank_spradley @HyperAHA A6/1. Placental DNA methylation and gene expression profile varies during pregnancy. Differential methylation as a cause or consequence of maternal blood pressure could not be distinguished due to the placentas being collected at term. #HYPHIP @HyperAHA

[Tsegaselassie Workalemahu @TWorkalemahu]

@Carmendemigue12 @WorkalemahuT @HyperAHA A4/2. In our study, increased third trimester SBP and DBP was associated with lower methylation of cg09735274 (TRIM31 gene) in placenta. #HYPHIP @HyperAHA

[Tsegaselassie Workalemahu @TWorkalemahu]

@fjorios @WorkalemahuT @HyperAHA @frank_spradley A5. We conducted genome-wide CpG methylation in the placenta, but it is possible to conduct candidate CpG methylation in other tissues. #HYPHIP @HyperAHA

[Tsegaselassie Workalemahu @TWorkalemahu]

@frank_spradley @HyperAHA A2. Sung et al Am J Hum Genet, 2018, has shown that reduced mRNA expression of collagen is involved in endothelial-to-mesenchymal transition in the heart endothelial cells in mice. #HYPHIP @HyperAHA

[Tsegaselassie Workalemahu @TWorkalemahu]

@alanephro @frank_spradley @WorkalemahuT @HyperAHA A1. Weaker expression of collagen was found in the intima of atherosclerotic plaques in humans and may play a role in preventing formation of atherosclerosis. #HYPHIP @HyperAHA