Andrew Borst

Head of Electron Microscopy, Institute for Protein Design @UWproteindesign, University of Washington @UW

[Andrew Borst @andrewjborst]

In particular, thank you to the other lead authors, @_JosephWatson, @naterbennett0, @RobertRagotte, @dejsee, Connor Weidle, @wanderingriti, and Yutong Yu!

This project is a testament to how computational and experimental science work together, each driving the other forward.

[Andrew Borst @andrewjborst]

Our updated preprint, "Atomically accurate de novo design of antibodies with RFdiffusion" is now available on BioArchive, now including scFvs!!

https://t.co/4W6Zg7ovkF

Shout-out to all our collaborators and co-lead authors. Computational antibody design and validation is HERE!

[Structura Biotechnology @structurabio]

Looking for a primer on why the contrast transfer function matters in #cryoEM?

Have a look at our updated #CryoSPARC Guide pages on CTF estimation! We cover intuition, theory, job types and practical considerations: https://t.co/9EPTdjZOI1

[Andrew Borst @andrewjborst]

Excited to share our latest pre-print! 🌟 Collaboration between @UWproteindesign & @NIH tackles the global threat of #influenza & the need for #H5N1 countermeasures. #CryoEM reveals how conserved motifs & water molecules help achieve broad protection.

https://t.co/uRsPcnkisD

[Andrew Borst @andrewjborst]

Big congratulations to Kenneth Carr and Dane Evan Zambrano, who co-led this study! 🎉 A special thank you to the developers of #ModelAngelo —this incredible tool was crucial to our success. Our designed proteins thank you! 👏

[Andrew Borst @andrewjborst]

Excited to share our latest pre-print outlining a successful use case of #ModelAngelo and protein BLAST in helping us solve a tricky protein purification challenge in computational #ProteinDesign!

@UWproteindesign @UWBiochemistry @cryoEM_Papers

https://t.co/tAbwjjg2jf

[Andrew Borst @andrewjborst]

Thanks to #CryoEM, #ModelAngelo', #BLAST, and #AlphaFold 3 predictions, we identified and modified our purification protocol to remove dihydrolipoamide succinyltransferase (hashtag#DLST) from our preps.

[Andrew Borst @andrewjborst]

We faced an issue with a unknown octahedral nanoparticle-like protein co-purifying with a number of our designed protein nanomaterials.

[The Veesler Lab @veeslerlab]

How is the human adaptive immune system responding to MERS-CoV infection? Find out by reading our @biorxivpreprint led by @AminAddetia
@HHMINEWS
@UWBiochemistry

1/16

https://t.co/pZlgbaZgR8

[ChimeraX (bsky: chimerax.ucsf.edu) @UCSFChimeraX]

CrAI, a tool using machine learning to automatically find antibodies in cryo-EM densities, is now available in ChimeraX (menu Tools / More Tools)! CrAI runs in seconds on a CPU, at resolutions up to 10A. Learn more at https://t.co/AVNsxRxgK2

[Joseph Watson @_JosephWatson]

We’re really happy to share our preprint demonstrating the atomically-accurate design of single-domain antibodies (VHHs) with RFdiffusion!

[bioRxiv @biorxivpreprint]

Atomically accurate de novo design of single-domain antibodies https://t.co/HGvmJVfY9Y #bioRxiv

[Andrew Borst @andrewjborst]

@UWBiochemistry @UWproteindesign @_JosephWatson @naterbennett0 @RobertRagotte I'd also like to express immense gratitude to my dedicated IPD Electron Microscopy Research Core team, Connor Weidle and Kenneth Carr, for their incredible contributions to towards this effort.

[Andrew Borst @andrewjborst]

Excited to share the release of our pre-print unveiling the design of de novo single-domain #antibodies with atomic-level precision, as revealed by #CryoEM! @UWBiochemistry @UWproteindesign

Work co-led by @_JosephWatson @naterbennett0 @RobertRagotte

https://t.co/kg5wZ6RD0b

[Andrew Borst @andrewjborst]

@UWBiochemistry @UWproteindesign @_JosephWatson @naterbennett0 @RobertRagotte A heartfelt congratulations to all co-authors, with special recognition to the other lead authors, @_JosephWatson, @naterbennett0, and @RobertRagotte for their outstanding leadership driving this project.

[Nate Bennett @naterbennett0]

We’re excited to share our preprint where we show, for the first time, the atomically accurate design of VHH antibodies!

[Institute for Protein Design @UWproteindesign]

We're hiring a Research Assistant (full-time, Seattle WA).

Main duties include protein characterization and electron microscope operation. Experience in protein structural biology is highly desired. Bachelor's degree required.

Learn more: https://t.co/xAu5KPsax2

[The Veesler Lab @veeslerlab]

I am honored to have been selected by @HHMINEWS to become part of the #HHMIInvestigators family. Grateful to my previous mentors as well as past/current trainees and collaborators. I am so excited about the science ahead of us!

[Andrew Borst @andrewjborst]

@veeslerlab @HHMINEWS Amazing!! Congratulations!!

[Andrew Borst @andrewjborst]

@irinakitop Sage-level wisdom and advice right here.