AnthoniG

Postdoctoral Fellow at Children’s Hospital of Philadelphia studying dentate gating properties in epilepsy. Always happy to chat systems neurosci and fungi.

[AnthoniG @AnthoniGoodman]

@AskAboutAnimals I very much doubt it. However I don’t doubt that it could be made.

[AnthoniG @AnthoniGoodman]

Great freeware for manual scoring of behavior: BORIS. Simple GUI and time-stamped point or state scoring. Idk why I had never heard of this during my PhD. Please pass on for any lab looking for basic but powerful behavioral software without coding.

[AnthoniG @AnthoniGoodman]

@DrLJBrady @UABHeersink @UABPsychiatry Congrats!!

[AnthoniG @AnthoniGoodman]

@mnitabach Rubus species

[AnthoniG @AnthoniGoodman]

@Roy_Lab_Thinks There was a really great podcast about this. Great story.

[AnthoniG @AnthoniGoodman]

@mari_scarduzio I’ve looked over vector builder and it looks like an ideal resource, I’m just not confident enough to design one myself and didn’t want to call until I exhausted other options 😝

[AnthoniG @AnthoniGoodman]

Does anyone know of an AAV/Lenti with a Chemogenic plasmid which is Deactivated [DO] by cre? DIOs are plenty popular but the opposite manipulation seems impossible to come by. CaMKii-driver is ideal.

[AnthoniG @AnthoniGoodman]

@Xander_PhD Great temp out this weekend too

[AnthoniG @AnthoniGoodman]

@nanopharmNC Also beware apps which monitor menstrual cycles and sell data (cough, Facebook, cough), as in those extreme states a missed period might be enough to justify investigation of ‘possible pregnancy’. 😬😳😮‍💨

[AnthoniG @AnthoniGoodman]

Also, I have too poor an understanding of plasmid construction to simply commission one without aid.

[AnthoniG @AnthoniGoodman]

For the record, I have several experiments which would greatly benefit from chemogenetically manipulating neurons without Cre (actively avoiding that population). A plasmid which can be put into a vector would also be amazing.

[AnthoniG @AnthoniGoodman]

Withdrawing a syringe following viral infusion is the most horrifically slow task. And that’s WITH a neuros 32guage. This needs to be automated.

[AnthoniG @AnthoniGoodman]

@LacyGoode The whole heckin abstract for me.

[AnthoniG @AnthoniGoodman]

@lindsay__alex Day by day

[AnthoniG @AnthoniGoodman]

Nothing makes me question my career path quite like a bad day at the rig. That desperation and mental agony is unbearable.

[AnthoniG @AnthoniGoodman]

@hardawayja Lenovo and HP workstations have performed well and lasted in my experience

[AnthoniG @AnthoniGoodman]

Looking for recommendations for fluorescent dyes which adhere to glass for patch pipettes at a depth where I lose significant resolution and need to visualize the very tip.

[AnthoniG @AnthoniGoodman]

@guanenst Always.

[AnthoniG @AnthoniGoodman]

@snkash @NickBoyleMSTP Okay so the Fluorospheres were excellent

[AnthoniG @AnthoniGoodman]

For practice stereotax surgeries I’m infusing cresyl violet, are there better markers?

[AnthoniG @AnthoniGoodman]

@vbprovencher I’ll give this a try! We have gobs of Dil in lab

[AnthoniG @AnthoniGoodman]

@NickBoyleMSTP Quickly sectioning afterwards and checking placement with dissecting scope