Postdoctoral Fellow at Children’s Hospital of Philadelphia studying dentate gating properties in epilepsy. Always happy to chat systems neurosci and fungi.
Great freeware for manual scoring of behavior: BORIS. Simple GUI and time-stamped point or state scoring. Idk why I had never heard of this during my PhD. Please pass on for any lab looking for basic but powerful behavioral software without coding.
@mari_scarduzio I’ve looked over vector builder and it looks like an ideal resource, I’m just not confident enough to design one myself and didn’t want to call until I exhausted other options 😝
Does anyone know of an AAV/Lenti with a Chemogenic plasmid which is Deactivated [DO] by cre? DIOs are plenty popular but the opposite manipulation seems impossible to come by. CaMKii-driver is ideal.
@nanopharmNC Also beware apps which monitor menstrual cycles and sell data (cough, Facebook, cough), as in those extreme states a missed period might be enough to justify investigation of ‘possible pregnancy’. 😬😳😮💨
For the record, I have several experiments which would greatly benefit from chemogenetically manipulating neurons without Cre (actively avoiding that population). A plasmid which can be put into a vector would also be amazing.
Looking for recommendations for fluorescent dyes which adhere to glass for patch pipettes at a depth where I lose significant resolution and need to visualize the very tip.