The Amit Choudhary Lab

Chemical biology, CRISPR and beta-cell biology @ Broad Institute | Harvard Medical School | Brigham and Women's Hospital

[Stephanie Wankowicz @stephanie_mul]

Sign to endorse: https://t.co/YdDjM24qlc

[Roland Dunbrack 🏳️‍🌈 @rolanddunbrack.bsky.social @RolandDunbrack]

Demis -- I think AlphaFold3 is really exciting. As Reviewer #3, I got great results from the server. I tried hard to get @Nature to urge you to release the code but was unsuccessful. I did not get it for re-review so I don't know if you responded. So why no code? @GoogleDeepMind

[Abhishek Chatterjee @AChemSynBio]

Thankful for this recognition. All credits go to my group's past and present members; I have been truly fortunate to work with them!

[Abhishek Chatterjee @AChemSynBio]

We got a story, and it’s electric! 🔌⚡️ https://t.co/K7w6MTOWzc
Can electrocatalysis be used to precisely modify a protein? Yes! Happy to finally share this work led by @conor_loynd and @SinghaSoumya. A 🧵 #ProteinElectrochemistry #NSFfunded

[ACS Biochem and ChemBio @acsbiol]

Congratulations to @CraigMCrews on receiving the Kimberly Prize from @NUFeinbergMed for pioneering studies of #PROTACs in the lab and their translation to clinical trials! Read more here: https://t.co/irihro1vlk

[David R. Liu @davidrliu]

The first in vivo base editing clinical trial outcomes are now reported, following last year’s positive ex vivo base editing clinical outcomes!
Congratulations to @kiranmusunuru , @skathire, @ambellinger, @NicoleGaudelli, & other scientists at @VerveTx and @BeamTx on these positive data.

[Dan Nomura @DanNomura]

New @biorxivpreprint from our lab by @Qian37774492 on a covalent 14-3-3 molecular glue with the transcription factors YAP and TAZ and using this glue as a 14-3-3 recruiter to sequester nuclear neo-substrates into the cytosol! Thanks to #AppleTreePartners & @themarkfdn
https://t.co/Vd2NXF6m0i

[Anthony Rullo: chemically modulating proximity @LabRullo]

Happy to share our most recent contribution coupling covalency with carbohydrate binding for immune proximity induction.
https://t.co/JA54P8OstH

[Laura Kiessling @ChemicalBiology]

I worry that this organization will put more pressure on reviewers to focus on the details and put even less focus on creativity and impact. That already seems to be an issue that was not addressed with these changes.

[The Amit Choudhary Lab @ChoudharyLab]

...we will report platform 2 design principles that enable recruitment of >310 kinases and lysine-based group transfer linkers as Lys is more abundant. These principles will help develop chimeras for writing/erasing any PTMs using inhibitors. Happy to share linkers/knowhow!

[The Amit Choudhary Lab @ChoudharyLab]

In @ACSCentSci, we report two chimeric molecule platforms to induce tyrosine phosphorylation, a critical PTM. Chimeras (formed by fusing target and kinase binders) force kinase to phosphorylate non-substrate via induced proximity
https://t.co/CaWUPNYlqY

[The Amit Choudhary Lab @ChoudharyLab]

Group transfer/proximity-induced labeling chemistries are well known (see Westheimer et al, Hamachi et al). Wyeth (2001) and AstraZeneca (2013) reported thiol-based group transfer for EGFR inhibitors, Thai et al (2020) have called it TMAcs and London et al (2021) CoLDR. Soon...

[William Gibson @wgibson]

What if small molecules could allow you to alter protein localization at will?

What if they could allow you to turn on specific transcription factors?

Our new manuscript reveals bifunctional molecules to do just that. 🧵

[Dan Nomura @DanNomura]

Excited to see our @ACSCentSci paper published on rational design of molecular glue degraders via a transplantable covalent handle! Congrats to @EthanToriki, @JPapatzimas, @NovartisScience, and team! Thanks also to @TheMarkFdn! @UCB_Chemistry https://t.co/i3VQ8d4q89

[Julia Joung @JuliaJoung]

Thrilled to share my primary PhD work: a Transcription Factor Atlas for understanding gene regulation and cell engineering @CellCellPress. We created a comprehensive TF ORF library and applied it to profile resulting expression changes. A thread 1/X https://t.co/PPcJ0HZEsW

[Jason Sheltzer @JSheltzer]

Very disappointed to see this new paper in @NatureCancer on a clinical trial with “HDAC6 inhibitor” ricolinostat. The paper fails to mention three recent reports demonstrating that this drug exhibits pervasive off-target toxicity.

[Milka Kostić, PhD @MilkaKostic]

The great @LynJonesChemBio and I partnered with an exceptional group of experts to bring you a book that talks about how thinking about protein homeostasis more holistically could help us find future medicines!
A gratitude 🧵