Jonathan Bouvette

Cryo-EM core NIEHS. Opinions are my own.

[Jonathan Bouvette @CryoJB]

Met amazing people at my first #3DEM @GRC this week!

For those who missed #3DEM, I am pleased to announce that I will be joining @Princeton @PrincetonMolBio in just 2 months as a core manager to lead the cryoEM efforts. Stay tuned for great science to come! #CryoEM #Teamtomo

[Jonathan Bouvette @CryoJB]

@ChargePointnet, I wasted over 3 hours this week waiting for service to pick up the phone. First, I waited 45 min for them to not help me. Then, I waited 30 min to schedule a callback that never happened. Tonight, i waited 75 min on the line... horrible service.

[Jonathan Bouvette @CryoJB]

@vikasnavaratna In grad school, we had made up new names for additional days when we didn't take time off during the weekend 😅. Just as if the week kept on going.

[Jonathan Bouvette @CryoJB]

@GlobalHigherEd It looks the same as the archives in the University of Montreal tower!

[Jonathan Bouvette @CryoJB]

@klausenhauser @IainWil92988866 When parties focus so much about playing the game and cheat as much as possible instead of having a good plan for fixing the issues.

[Jonathan Bouvette @CryoJB]

@cryomariena You should get a hole punch from @SubangstromLLC. They're great and you will never have that problem again.

[Jonathan Bouvette @CryoJB]

@GntlmnScientist @mike_feigin That sounds similar to the traditional French Canadian sugar shack all-you-can-eat brunch tradition.

[Jonathan Bouvette @CryoJB]

@LanderLab @Bex_16 @GrigorieffLab @cryosparc I already pulled it out of the archive to add it in https://t.co/pSGePycEtH. Ice thickness and tilt will be nice additions!

[Jonathan Bouvette @CryoJB]

@klausenhauser That reminds me of high school. Same tiles also.

[Jonathan Bouvette @CryoJB]

@Nexperion @sonjawelsch It's been working quite weel on Arctica and Krios G3/G4 on Win7 and Win10.
I still left the option to disable the aperture commands in case of problems or for older scopes that don't have these options.

[Jonathan Bouvette @CryoJB]

#CryoEM SmartScope v0.9.2 is out!
Here are a few upgrades:
- Aperture Control w/ SerialEM 4.1-beta @Nexperion
- Great progress on CryoARM support.
- More flexibility about data storage locations.
- Easier installation procedure.

Full notes at: https://t.co/i1LF21BJx4

[Jonathan Bouvette @CryoJB]

Please check out @scipion_em smartscope plugin to feedback your preprocessing data to SmartScope.

Now in Beta!

https://t.co/AZihTexmum

[Jonathan Bouvette @CryoJB]

I received a broken litre of hot sauce from @Walmart and other items that got ruined during shipping

No offer to reship nor honor the prices at the time of purchase

They offered a refund- that's it

#doneWithWalmart
#CustomerExperience
Wouldn't have happened @Target

[Jonathan Bouvette @CryoJB]

@paultkim_ipd I Jedi mind-tricked them back with @onbeeper. Now I have a blue bubble from Android.

[Jonathan Bouvette @CryoJB]

@GntlmnScientist Yes but only in packs of 4.

[Jonathan Bouvette @CryoJB]

@ATinyGreenCell I don't think Bluetooth is a good protocol to dive into if your controller has WiFi. You'll have access to many more protocols for moving the data around.

[Jonathan Bouvette @CryoJB]

@klausenhauser Just to fill the gap before raclette season. Just need another "cook as you eat" meal?
I'd personally use this meat to make steak subs.

[Jonathan Bouvette @CryoJB]

@alexjamesnoble For all I know it's a play on Pipeline. Should be pronounced like Pipe (PYP).

[Jonathan Bouvette @CryoJB]

From having first-hand experience with this software, it is incredible.

The tomo part particularly, fills a gap in the field. Never build a tomogram manually again.

And for SPA preprocessing, you can't get faster than this with parallelization up to your cluster limits.

[Nature Methods @naturemethods]

Out today from the Bartesaghi lab! nextPYP is a turn-key framework for single-particle cryo-ET that streamlines complex data analysis pipelines, from pre-processing of tilt series to high-resolution refinement, for efficient analysis of large datasets. https://t.co/ot2wR3wbFO

[Jonathan Bouvette @CryoJB]

@vikasnavaratna If you have access to a printer, you can achieve this goal.

[Jonathan Bouvette @CryoJB]

@tiangolo @ThePSF @jetbrains @FastAPI I use Django and at this point, I'm too far in to switch. However, I've been playing with fastAPI on the side and getting ready for my next projects