Sagar

Fiddling with the code and instruments for CryoET in between coffee, good food. Opinions are my own.

[Sagar @CryoSagar]

Hey #teamtomo checkout our team science approach to #cellbio #cryoET

I can’t thank @bengeliscious enough for this one 😍😍

Awesome team science from @MPI_Biochem @biozentrum @thermosciEMSpec and more

Signing off Twitter with a good one

https://t.co/r4tT7jTUgW

[Sagar @CryoSagar]

@AlisterBurt @bengeliscious @MPI_Biochem @biozentrum @thermosciEMSpec Yeahh!!! 🥳 that was some fun getting TOMOMAN-STOPGAP-Warptools pipeline go brrr 😂

[Sagar @CryoSagar]

Hey #teamtomo checkout our team science approach to #cellbio #cryoET

I can’t thank @bengeliscious enough for this one 😍😍

Awesome team science from @MPI_Biochem @biozentrum @thermosciEMSpec and more

Signing off Twitter with a good one

https://t.co/r4tT7jTUgW

[Sagar @CryoSagar]

Order in Chaos brought to you by #TOMOMAN with generous support from @williamnwan #teamtomo #cryoet #insitu #opensource

https://t.co/XsBXP8EZzU

[Florent Waltz @FlorentWaltz]

Same for @CryoSagar and STOPGAP, which was instrumental here. But it still remains really difficult to make this kind of analysis because the processing pipeline is not as streamlined as in SPA. Actually, sometimes it really looks like that:

[Structural Biology @ActaCrystD]

William Wan et al.: STOPGAP: an open-source package for template matching, subtomogram alignment and classification #STOPGAP #CryoET #OpenSource @vanderbiltu... #IUCr https://t.co/tbgA39W6Eu

[Sagar @CryoSagar]

#STOPGAP is now published 🥳🥳
With @williamnwan and Twitter less Jonathan Wagner
I must say, the peer review @ActaCrystD was as it should be, relevant, fast 😍
One of many to be published together with awesome @williamnwan
#teamtomo #opensource #cryoEM

[Will Wan @williamnwan]

Our manuscript for STOPGAP is now out in @ActaCrystD! It was great working with @CryoSagar
and twitterless Jonathan Wagner.

https://t.co/WEFCjsauYF

[Alister Burt @AlisterBurt]

When I joined David Barford's group @MRC_LMB it was with the goal of using my cryo-ET and computational skills to help them understand funky biology. I joined @TDendooven and @StanislauY's efforts to average the native gamma tubulin ring complex (γTuRC) from tomograms of SPBs

[Sagar @CryoSagar]

Now all we need some is #communitycompute to mine all this data together! And visualize life at molecular detail! #cryoET goes #bigdata
Any thoughts from awesome people at @GoogleDeepMind @AmazonScience @nvidia @NVIDIAAI
time to go brrrrr!!!!

[Sagar @CryoSagar]

Hey #teamtomo Here is EMPIAR-11830!
Some 1800 tomograms from awesome #Chlamy (Chlamydomonas reinhardtii) using large scale #cryoET!
In addition , all the metadata and preprocessing as #minimalproject to get you started right away! #TOMOMAN #bigdata #communityscience 1/

[Sagar @CryoSagar]

Made possible by massive efforts @thermosciEMSpec @MPI_Biochem @maxplanckpress @biozentrum

[Sagar @CryoSagar]

Hey #teamtomo Here is EMPIAR-11830!
Some 1800 tomograms from awesome #Chlamy (Chlamydomonas reinhardtii) using large scale #cryoET!
In addition , all the metadata and preprocessing as #minimalproject to get you started right away! #TOMOMAN #bigdata #communityscience 1/

[EMBL-EBI @emblebi]

The new @EMDB_EMPIAR cryo-electron tomography (cryo-ET) browser allows you to see proteins up close in the cellular environment.

Over 1,800 high-resolution reconstructed tomograms from cryo-plasma-FIB milled Chlamydomonas reinhardtii cells are available.

https://t.co/YTgnVvn46i

[Sagar @CryoSagar]

Congrats @svenklumpe 🥳🥳
This map looks so cool! I think this was and is one of those discovery moments in cryo-ET!

[Sven Klumpe @svenklumpe]

Over 4 years in the making, I am excited to share our pre-print on the “In-cell structure and snapshots of copia retrotransposons in intact tissue by cryo-electron tomography”. We used cryo-ET to resolve the copia capsid structure to subnanometer resolution inside cells. A 🧵

[Sagar @CryoSagar]

@williamnwan I must say that this wouldn’t be possible without the support from @MPI_Biochem @maxplanckpress @VanderbiltU

[Sagar @CryoSagar]

Hey #teamtomo!! you can finally cite it properly! After ‘a while’ in making (mostly my fault🤣) , discussions on methods, philosophy, etc. with @williamnwan 🥳 it’s been a fun ride and I can only say look out for more 🤓😛

[Will Wan @williamnwan]

Just in time for Festivus, our preprint on STOPGAP is out! In it, we describe the subtomogram averaging algorithms we find useful. It was great working on this with @CryoSagar and twitterless Jonathan Wagner.
https://t.co/AK4uEm0shf

[Sagar @CryoSagar]

@QiangGuo_EM @PNASNews This is awesome 🤩 congrats 🎉

[Sagar @CryoSagar]

Just go for it! Don’t blink ;) Cool stuff with even cooler scientists!!

[Wim Hagen @WimJHH]

My indispensable right hand Natalya Dudkina is hiring, join our exciting R&D group at Thermo Fisher Scientific as Application Engineer, Cryo-EM.

RT please!

https://t.co/N60h1RJ7CM

[Sagar @CryoSagar]

@bengeliscious @A_Amunts @AbhayKot @thermofisher @FlorentWaltz @EMDB_EMPIAR @williamnwan The future is @Arm🤓🤓

[Sagar @CryoSagar]

@bengeliscious @A_Amunts @AbhayKot @thermofisher @FlorentWaltz @EMDB_EMPIAR Indeed! This project is a marathon 💪
But thanks to an amazing partnership with @williamnwan for #TOMOMAN and #STOPGAP, we can dream of taking on such daunting tasks 🥳🥳🥳
Especially our recent efforts in #TOMOMAN has been quintessential for this project 😇😇 #opensource

[Sagar @CryoSagar]

When @AbhayKot and I started this thing, this is what we envisioned! Thanks for the support from JP, @MPI_Biochem and @thermosciEMSpec we can now see the fruits ☺️ can’t wait for the release with @EMDB_EMPIAR! It’s been even more fun with @bengeliscious and team joined😍

[Sagar @CryoSagar]

@QiangGuo_EM @MPI_Biochem @thermosciEMSpec Thank you @QiangGuo_EM ☺️ hoping to see you sometime sooner 😊

[Sagar @CryoSagar]

From Am Klopferspits 18 @MPI_Biochem to Achtseweg Noord 5 @thermosciEMSpec 😉 slowly making it to the mothership!

[Sagar @CryoSagar]

@mdickmanns1 @MPI_Biochem @thermosciEMSpec They say a GIF says more than words! 🤓

[Sagar @CryoSagar]

@MPI_Biochem @williamnwan @3P1L And of course over past couple years collaborating with @AbhayKot and the team at @thermosciEMSpec has been amazing!
Now looking forward to amazing science we will do together @thermosciEMSpec 😍
I think we are baking some cool things;)

[Sagar @CryoSagar]

It’s been Amazing 5+years @MPI_Biochem with Juergen Plitzko! I have been fortunate to have great mentors as JP, @williamnwan, @3P1L. It’s been fun hanging out with and doing cool stuff with the Martinsried peeps and looking forward to more;)