Same for @CryoSagar and STOPGAP, which was instrumental here. But it still remains really difficult to make this kind of analysis because the processing pipeline is not as streamlined as in SPA. Actually, sometimes it really looks like that:
William Wan et al.: STOPGAP: an open-source package for template matching, subtomogram alignment and classification #STOPGAP#CryoET#OpenSource@vanderbiltu... #IUCr https://t.co/tbgA39W6Eu
When I joined David Barford's group @MRC_LMB it was with the goal of using my cryo-ET and computational skills to help them understand funky biology. I joined @TDendooven and @StanislauY's efforts to average the native gamma tubulin ring complex (γTuRC) from tomograms of SPBs
The new @EMDB_EMPIAR cryo-electron tomography (cryo-ET) browser allows you to see proteins up close in the cellular environment.
Over 1,800 high-resolution reconstructed tomograms from cryo-plasma-FIB milled Chlamydomonas reinhardtii cells are available.
https://t.co/YTgnVvn46i
Over 4 years in the making, I am excited to share our pre-print on the “In-cell structure and snapshots of copia retrotransposons in intact tissue by cryo-electron tomography”. We used cryo-ET to resolve the copia capsid structure to subnanometer resolution inside cells. A 🧵
Hey #teamtomo!! you can finally cite it properly! After ‘a while’ in making (mostly my fault🤣) , discussions on methods, philosophy, etc. with @williamnwan 🥳 it’s been a fun ride and I can only say look out for more 🤓😛
Just in time for Festivus, our preprint on STOPGAP is out! In it, we describe the subtomogram averaging algorithms we find useful. It was great working on this with @CryoSagar and twitterless Jonathan Wagner.
https://t.co/AK4uEm0shf
My indispensable right hand Natalya Dudkina is hiring, join our exciting R&D group at Thermo Fisher Scientific as Application Engineer, Cryo-EM.
RT please!
https://t.co/N60h1RJ7CM
When @AbhayKot and I started this thing, this is what we envisioned! Thanks for the support from JP, @MPI_Biochem and @thermosciEMSpec we can now see the fruits ☺️ can’t wait for the release with @EMDB_EMPIAR! It’s been even more fun with @bengeliscious and team joined😍
It’s been Amazing 5+years @MPI_Biochem with Juergen Plitzko! I have been fortunate to have great mentors as JP, @williamnwan, @3P1L. It’s been fun hanging out with and doing cool stuff with the Martinsried peeps and looking forward to more;)