David Halfmaerten

Research Assistant Genetic Diversity @INBOVlaanderen working on #eDNA #metabarcoding fish and amphibians

[David Halfmaerten @DavidACGT]

@JELittlefair @alpineedna @Dr_bat_girl @kristinebohmann @taylormwilcox @stefanako71 @ShrewGod We are using Incubator-Genie SI-1402 https://t.co/jJEO9g1y1o

[Tim Adriaens @AdriaensTim]

curious about what is going on with #Xenopuslaevis in Flanders blogpost by Marco Morbidelli @TricaricoElena https://t.co/gefFsiqs5z

[Charlotte Van Driessche @Vdriecha]

Today we are presenting our most recent research outputs at the International Conference on Aquatic Invasive Species in Ostend, Belgium. Proud to be a member of this enthusiastic research team! #ICAIS2022 @FWOVlaanderen @INBOVlaanderen #eDNA #eTeam

[Charlotte Van Driessche @Vdriecha]

Congrats to my colleague for his excellent work on the innovative #eDNA #ddPCR research: you deserve this Positive Droplet Award, Rein! 👏👏
@INBOVlaanderen @BioRadLifeSci
https://t.co/10nN6UHXVb

[David Halfmaerten @DavidACGT]

Our @INBOVlaanderen lab and innovative #eDNA #ddPCR research led by Rein Brys is in the running for a Positive Droplet Award by @BioRadLifeSci https://t.co/qG78VdvmY3 Don't forget to cast your vote this week for your favorite nominee!

[David Halfmaerten @DavidACGT]

Must-read #OpenAccess guide for everyone embarking on #eDNA assessments and get answers to your FAQ. So glad to see first edition out from this amazing @DNAquaNet group effort that should stay updated as the field evolves!👏

[COST Action CA15219 @DNAquaNet]

👉Practical guide for #DNA #eDNA assessments published! 🍾
Amazing effort by @DNAquaNet @COSTprogramme members. Finally out: https://t.co/PdFLD2GOUR
And yes, the methods continue to evolve - but this provides very good contours & guidance, a lot consensus on #eDNA #metabarcoding

[David Halfmaerten @DavidACGT]

Now open access in early view

[Annelies Haegeman @annelies_haegem]

#eDNA emission from fish / amphibians is very local and temporary, stressing the importance of a smart sampling strategy in water-based biodiversity monitoring. Read our latest paper from @INBOVlaanderen and @ILVOvlaanderen here👇🏻https://t.co/j0cDwCUpbF

[Tim Adriaens @AdriaensTim]

checking for #Xenopuslaevis presence in ponds after discovery in France and Wallonia using #eDNA #invasivespecies #fieldworkincovidtimes

[Dr Kristy Deiner @alpineedna]

#ReinBrys https://t.co/MJFBoWbQWM using creative #eDNA methods for understanding detection of the weather loach in Flanders. Dispersion experiments, ddPCR overcoming inhibition challenges #FBSI19

[Lori Handley @Calvia14]

Fab demonstration of how eDNA can detect elusive weather loach & inform reintroduction efforts by Rein Brys from @INBOVlaanderen at #FSBI19 ... ddPCR much better than qPCR for overcoming inhibition

[David Halfmaerten @DavidACGT]

@VascoElbrecht @lynseyrharper @BerndHaenfling @alpineedna @eDNAScience @DNAquaNet @SiCreerProf @cristinadimuri @metagusano @KevinBeentjes @luke_earl @Gnarly_Larvae Thanks Vasco! We hope lots of eDNA users will follow our recommendations on the use of internal positive controls and as @KevinBeentjes stressed report very well on the methods so we can combine everyone's results to further refine consensus on eDNA standard and best practices.

[David Halfmaerten @DavidACGT]

@taylormwilcox Actually the IPC qPCR assay we are using has been published in the dutch standard NEN 6254:2012 protocol for Legionella pneumophila detection which unfortunately is in dutch language and not free. https://t.co/unku5ahVvx

[David Halfmaerten @DavidACGT]

You've done a hell of a job @Johansviswereld with a more than deserved happy ending! Keep up the good work 👏. https://t.co/1i7u86sRfh

[Johan Auwerx @Johansviswereld]

Today we'll release 263 cultured newts into the wild. There will be more next weeks. These are the happiest moments, when those small creatures are leaving the farm. #ilovemyjob
#reintroduction #trituruscristatus #kamsalamander #greatcrestednewt @INBOVlaanderen @Amphibian_Ark

[David Halfmaerten @DavidACGT]

No i haven't written them but no wonder lots of people refer to the first primer set as Kelly primers since @Invertdna did refer to them very well in the M&M section of his 2014 edna fish mesocosm pub https://t.co/BikSmhJI7e https://t.co/4WnGGio5ub

[Dr Kristy Deiner @alpineedna]

@DavidACGT Arrgh, I bet you wasted a day to figure these errors out. Thanks for adding to the list! I wonder how many errors are out there like this? The ones in Riaz (2011) seem worthy of a published correction! Have you written to them?

[David Halfmaerten @DavidACGT]

@alpineedna Well good luck if you start aligning vertebrate primers from original Riaz (2011) pub that don't even have a decent name, first primer pair is labeled 16S instead of 12S and for 2nd primer pair direct and reverse primer are switched in wrong column 😱

[David Halfmaerten @DavidACGT]

Autopsy and genetic research @INBOVlaanderen on Flanders' second and unfortunately road-killed wolf. https://t.co/H26SiJax6C

[Lori Handley @Calvia14]

@G_S_Sellers from @EvoHull presents mu-DNA - a modular dna extraction toolkit for eDNA and beyond. It works and gives you more science for your money!! #UKDNAWG

[Prof. Michael Sweet @DiseaseMatters]

One of @DerbyUni project partners Rein Brys talks to us about eDNA from fish in freshwater systems - how far can we get? #UKDNAWG

[Colin Bean @ProfColinBean]

Tour de force from Rein Brys - DNA persistence+detection from souce has signif implications for monitoring rare fish #UKDNAWG @SNH_Tweets