Sam Horrell 🗿

Structural Biology Facility Manager @ImperialCollege. Expect protein crystallography🧬💎🎇, Boardgames♟️, D&D🎲, MTG🧙, and birds called Nori and Kili 🦜🦜

[Sam Horrell 🗿 @DrHorrell]

I found a folder of pretty protein #crystal pictures so... here you go. 💎🧵

[Sam Horrell 🗿 @DrHorrell]

This account will be left here for posterity but will no longer be used actively, unless there is a significant change in the direction of the community/ management of the site. You can find me where the skies are blue under the same username.

Have a parting crystal picture

[Sam Horrell 🗿 @DrHorrell]

Love it when an @OxfordBusCo bus leaves early instead of waiting until the scheduled departure time so your left waiting for 30 minutes for the next bus.

This is where the bus was at 19:22 when it should have been where I am.

[Sam Horrell 🗿 @DrHorrell]

@bunsenbernerbmd I stand with Ginger.

[Sam Horrell 🗿 @DrHorrell]

Excuse me sir... The scritches appear to have stopped. This must be a mistake.

[Sam Horrell 🗿 @DrHorrell]

@WarSpog It's the crystal structure of a DNA binding protein that I've generated the symmetry mates of to reconstruct what the crystal actually looks like.

[Sam Horrell 🗿 @DrHorrell]

Can't imagine why crystallising this without DNA has been so difficult.

[Sam Horrell 🗿 @DrHorrell]

Also, looking at this crystal structure makes me feel like the hyper drive has just been activated.

[Sam Horrell 🗿 @DrHorrell]

$540 worth of bases recovered from the pin graveyard.

[Sam Horrell 🗿 @DrHorrell]

Reduce. Reuse. Recycle.

Reanimating bases from the broken pin graveyard today.

[Sam Horrell 🗿 @DrHorrell]

@GntlmnScientist

[Sam Horrell 🗿 @DrHorrell]

Reduce. Reuse. Recycle.

Reanimating bases from the broken pin graveyard today.

[Sam Horrell 🗿 @DrHorrell]

@Sci_Hans Not quite so bad anymore, $3 by the looks of it. Gotta be over 100 bases here though.

[Sam Horrell 🗿 @DrHorrell]

@serialstubbs @GntlmnScientist @DougInstruments Yeah, the user I helped with this used the mosquito to dispense 20 nl of the seeds. Harvested crystal clusters from 8 wells, crushed them with glass beads, then made 8 serial dilutions to be dispensed. I think it was the 100 and 1000 fold dilutions that worked best.

[Sam Horrell 🗿 @DrHorrell]

Before seeding | After Seeding

[Sam Horrell 🗿 @DrHorrell]

@GntlmnScientist No cat whiskers were harvested in the production of these crystals. Should my parents cat ever drop one while I'm around I'm definitely pocketing it though.

[Sam Horrell 🗿 @DrHorrell]

@GntlmnScientist Second option would be colour it black and you've got a Darth Vader helmet. Or possibly a Masked Rider if you want a 90s nostalgia hit.

[Sam Horrell 🗿 @DrHorrell]

@GntlmnScientist Naturally, no way you're crystallising Slimer like that.

If you colour the alphas helix bit pink/red for his mouth I think the portrait would be nearly complete.

[Sam Horrell 🗿 @DrHorrell]

@Samuel_Gregson @theory_dad I don't think there's anything that can't be imporved by adding in some Pedro Pascal. He even diffracts don't you know!

https://t.co/u0PRU4zxqB

[Sam Horrell 🗿 @DrHorrell]

Pedro Pascal as protein crystals, Pedro crystals, and Pedro diffraction. A Thread.

[Sam Horrell 🗿 @DrHorrell]

@Sci_Hans The church of seeding!

[Sam Horrell 🗿 @DrHorrell]

@Samuel_Gregson @theory_dad I think you'll find the origin of flavour is flavour town.

[Pete Smyth @Pete_Smyth_]

My contribution to Methods in Enzymology with Katerina and Joachim is available online:
https://t.co/piqouhyFck
@EuropeanXFEL

[Sam Horrell 🗿 @DrHorrell]

@klausenhauser Not sure on the distinction, for this one I had to transfer a crystal from an old drop to a new one to get big atomic resolution diffracting crystals.

Were you just streaking with a whisker without introducing another crystal from elsewhere?