Hasan Mamar

PhD student studying ADP-ribosylation at Gyula Timinszky's lab.

[Hasan Mamar @HasanMamar]

@sihemzenbio @PNASNews @IGDRennes @SprteVillejean1 Congratulations Siham! 🎊 🥳

[Hasan Mamar @HasanMamar]

@AsAshraf123321 @NAR_Open Thank you @AsAshraf123321 !

[Hasan Mamar @HasanMamar]

Excited to see my PhD work published @NAR_Open, describing an HR-independent #PARPi sensitivity upon loss of #polymerase epsilon accessory subunits POLE3-POLE4.
POLE4 loss enhances sensitivity of #BRCA1-deficiency & bypasses resistance due to 53BP1 loss.
https://t.co/9ut4jHT5B0

[Hasan Mamar @HasanMamar]

@Ella_Maru @NAR_Open Thank you @Ella! While we haven't tested it directly yet, we think it's due to POLE4 ensuring stable binding of polymerase complex to DNA. Upon its absence, continuous repriming leaves gaps accumulating behind the fork, exacerbating replication stress in BRCA1-deficient cells.

[Hasan Mamar @HasanMamar]

@bexsmithnz2 @BiologicalRese1 @SprteVillejean1 @sihemzenbio @bowchro @BiochemOxford Thanks a lot @bexsmithnz2

[Hasan Mamar @HasanMamar]

@sihemzenbio @bexsmithnz2 @BiologicalRese1 @SprteVillejean1 @bowchro @BiochemOxford Thank you @sihem!

[Hasan Mamar @HasanMamar]

@MichaelNADbio @NAR_Open Thank you so much @Michael!

[Hasan Mamar @HasanMamar]

Immensely grateful to my supervisor Gyula Timinszky & Roberta Fajka-Boja (shared 1st author) for unforgettable journey.

It was a pleasure collaborating with Haracska lab (@BiologicalRese1) @SprteVillejean1, @bexsmithnz2, @sihemzenbio, @bowchro & Lakin lab (@BiochemOxford)

[Hasan Mamar @HasanMamar]

#PARPi leads to accumulation of ssDNA gaps in POLE4 KO, due to impaired post-replicative repair. This induces #replicationstress phenotypes mediated by ATR and DNA-PK.
Consequently, POLE4 KO are hypersensitive to ATRi and its combination with PARPi.

[Hasan Mamar @HasanMamar]

@sihemzenbio Congrats Siham! Have fun in NY!

[Hasan Mamar @HasanMamar]

@bowchro Tesco, et al.

[Hasan Mamar @HasanMamar]

Grateful to @ANoireterre for preparing this @preLights digest of our latest #preprint
#ADPribosylation #PARP

[preLights @preLights]

How to make cancer cells more sensitive to treatment. 💊

Preprint from @HasanMamar & team shows that the loss of POLE4 - component of POLε - contributes to PARPi sensitivity, regardless of BRCA status.

First preLight from @ANoireterre, check it out! 👀

https://t.co/8wnZDAqYzq

[Hasan Mamar @HasanMamar]

@DangH_Nguyen Thanks a lot! I appreciate taking the time to read it.

[Hasan Mamar @HasanMamar]

Thrilled to see my first first-author PrePrint online 😃, describing an HR independent sensitivity to PARPi upon deletion of accessory subunits of DNA pol epsilon.

Grateful to work with Roberta Fajka-Boja(co-first) and my supervisor Gyula Timinszky
#PARP #ADPribosylation

[bioRxiv Cell Biology @biorxiv_cellbio]

The loss of DNA polymerase epsilon accessory subunits POLE3-POLE4 leads to BRCA1-independent PARP inhibitor sensitivity https://t.co/Bzjo75qSRO #biorxiv_cellbio

[Hasan Mamar @HasanMamar]

@MichaelNADbio Thank you so much for the support Mike!

[Hasan Mamar @HasanMamar]

@SprteVillejean1 Thank you so much!

[Hasan Mamar @HasanMamar]

@bowchro Thanks a lot Andrew!

[Hasan Mamar @HasanMamar]

@sihemzenbio Thank you Dr. Siham! 😉

[Hasan Mamar @HasanMamar]

@_ayybengulerim Thanks a lot Fatma!

[Hasan Mamar @HasanMamar]

Another fun work with our friends @SprteVillejean1 @sihemzenbio & @bexsmithnz2

[Hasan Mamar @HasanMamar]

Big thanks to all my colleagues at Timinszky lab who aren't on X.
And to the Haracska lab our collaborators at @BiologicalRese1