@profjeffchan@LukeshGroup@WakeForest@WakeChemistry That's great to hear! He's a great chemist! And apart from 7-Hydroxycoumarin and Resorufin, we did not try other dyes. Perhaps more dye variation will be a feat we attempt in subsequent studies! Thank you for viewing!
@mdpluth@LukeshGroup@WakeForest@WakeChemistry Thank you for viewing! Good question- While we did not study the exchange rate for a particular thiol species, we do know that in the presence of H2S, it cyclizes quickly to fully release 7-hydroxycoumarin in under 2 minutes!
@tnewt116 That's a great question actually. We certainly were not looking for those byproducts but since the fluorescence is retained in the presence of both H2S and GSH, and thereby regeneration of the probe (Fig. 3A), I would say GSH alone is generated without Se.