Joe Lam

PhD candidate | The University of Hong Kong | RNA | CRISPR

[Joe Lam @KaiChing123]

(1/6) We are so excited to share our latest work on CRISPR-Cas13 in @NatureComms!
We unveiled the cleavage mechanism of Cas13 and established the RNA Segment Editing (RSE), a targeted RNA cleavage and repair tool.
https://t.co/KkJzn6EDuq
A thread 🧵

[HKU Medicine @hkumed]

Researchers from @hkusbms have developed a novel ‘cut-and-patch’ tool for RNA, paving the way for a new generation of reversible, targeted therapies for neurodegenerative conditions such as Huntington’s disease.

https://t.co/9A39j8gNaK

[Joe Lam @KaiChing123]

@schul_kwon @JA_YKLi @Jon_wkkongaa (6/6) We envisioned the RNA Segment Editing could have broad applications in RNA therapeutics and basic research. I am very grateful for my mentor @schul_kwon and all members of the Chul Lab who helped me along the way. @skleungah @ezcheng1111 @JA_YKLi @Jon_wkkongaa and Jeffery.

[Joe Lam @KaiChing123]

(1/6) We are so excited to share our latest work on CRISPR-Cas13 in @NatureComms!
We unveiled the cleavage mechanism of Cas13 and established the RNA Segment Editing (RSE), a targeted RNA cleavage and repair tool.
https://t.co/KkJzn6EDuq
A thread 🧵

[Joe Lam @KaiChing123]

(5/6) Finally, we repurposed the in vivo adaptor ligation method as a targeted RNA cleavage and repair tool (RNA Segment Editing). As a proof of concept, we use an out-of-frame GFP reporter and only in the presence of both donor RNA and targeting crRNA will the GFP be restored!

[Joe Lam @KaiChing123]

(4/6) Next, with the help of single-nucleotide-resolution mapping of cleavage sites, we show that we could modulate the cleavage preference of PspCas13b by mutating certain residues near the HEPN active sites, potentially increasing its specificity!

[Nick Desnoyer @NickDesnoyer]

"Imagination is everything. It is the preview of life's coming attractions" -Albert Einstein

[Andrey Tvardovskiy @ATvardovskiy]

https://t.co/n1rr4BmoQs

[NatureStructMolBiol @NatureSMB]

New online! Computational design of sequence-specific DNA-binding proteins https://t.co/8dMUtDhPep

[Chul KWON @schul_kwon]

Cytosolic CRISPR RNAs for efficient application of RNA-targeting CRISPR-Cas systems | EMBO reports https://t.co/5s2KPR2S88 Our paper is out in @emboreports! 1/n

[Chul KWON @schul_kwon]

We are proud to present cytosolic Cas13 crRNAs driven by the U1 promoter. If you are using NES-fused Cas13 or Csm systems, try to use this crRNA expression system, which is robust and superior to the conventional U6 promoter. https://t.co/9hSbBQUmfU @biorxivpreprint

[Charles @charlcwf]

Enabling the surveillance and therapeutics development at speed for syncytial viruses like SARS-CoV-2 https://t.co/igfXscfnap

[george church @geochurch]

https://t.co/9VsdyTL6JF

[Cell Press @CellPressNews]

Researchers find that body odor is critical for #mosquitoes to find humans over long distances, using what researchers say "is the largest system to assess olfactory preference for any mosquito in the world." 🧵 1/7 https://t.co/OobCvoa0WM

@dags263 @stephrankin2 @McMenimanLab

[Ricard Solé @ricard_sole]

Soft-bodied marine animals have evolved all kinds of adaptations. Why do complex brains show up only in cephalopods? Why don't we see complex neural architectures in jellyfish? This is my drawing (the last version) of a brainy jellyfish. A missing piece in the cognitive biosphere

[Lining Yao @lining_yao]

Inspired by nature, sourced from nature, designed for nature, and published in @Nature ! Biodegradable morphing robots are "gone to earth".

[Paul Stamets @PaulStamets]

What an incredible video that was captured of mushrooms releasing spores into the wind! Nature is spectacular. 

Video credit: Villareal C. Jojo

[Historic Vids @historyinmemes]

Queen Elizabeth Il on Algebra, 2011