Michael Kaminski Lab

MDC/BIMSB
Cell Fate Engineering and Diagnostics

[Michael Kaminski Lab @KaminskiLab]

Our work on base editing for correction of pathogenic variants causing #ADPKD is now published at @MolTherapy

https://t.co/sRWn2hzwt9

Since the pre-print, we added some new interesting findings. Brief thread (1/3)👇

[Katalin Susztak @KSusztak]

🚨 Our new paper just dropped !!! Proteomic risk score for early prediction of kidney disease progression in individuals with APOL1 high-risk genotypes https://t.co/4WCXVKst5S

[Rafael Kramann, MD PhD @rkramann]

Our new paper in @naturemethods led by @vanvanka123 @MingboCheng Jitske Jansen with ⁦@KuppeChristoph⁩ https://t.co/h5atAwaP6z

[Can Dincer @CanDincer83]

🚀 Excited to share our new Perspective "Convergence of #nanotechnology and #CRISPR-based #diagnostics" in Nature Nanotechnology🤩

https://t.co/DhsorjgRKk

#HealthcareInnovation #Wearables #Biosensors

[Katalin Susztak @KSusztak]

🚨 Kidney + AI milestone 🚨
Never been more excited to share!!!
We built Nephrobase Cell+, the first multispecies, multimodal kidney foundation model:
Trained on ~40M cells
Across 4 species (human, mouse, rat, pig)
From 5 modalities (scRNA, snRNA, snATAC, CosMx, Xenium)
Kidney biology finally has its own GPT moment.👇

[Andreas Linkermann @Linkermann_Lab]

Glad to share our open access nature paper. Congrats to the three first authors and thanks to everyone involved!
https://t.co/j3A1j5D5VJ

[Paola Romagnani @PRomagnani]

It started by chance: in our all-women lab, only urine from 2 pregnant colleagues grew renal progenitors. Why? After 10 yrs, the answer is now in Science. Grateful to our brilliant first author @CarolinaConte5 from @UNI_FIRENZE who led the way! 👏 https://t.co/P2ATMuUCa6

[Michael Kaminski Lab @KaminskiLab]

Many thanks to our first authors Antonia Ibel & Rishi Bhardwaj @rishipu89, and the entire team. Joint effort with Sorin Fedeles @YaleMed and Matteus Krappitz @CUAnschutz

[Michael Kaminski Lab @KaminskiLab]

Our work on base editing for correction of pathogenic variants causing #ADPKD is now published at @MolTherapy

https://t.co/sRWn2hzwt9

Since the pre-print, we added some new interesting findings. Brief thread (1/3)👇

[Michael Kaminski Lab @KaminskiLab]

Deglycosylation analysis of PC1 showed similar fractions of mature (>90%, Endo H-resistant) and immature (Endo H-sensitive) glycoforms in treated vs. control cells, indicating rescue of the mature isoform by base editing. (3/3)

[Molecular Therapy Family of Journals @MolTherapy]

In vivo base editing reduces liver cysts in autosomal dominant polycystic kidney disease https://t.co/3NTKQ3975G #genetherapy #celltherapy

[Ben Kleinstiver @BKleinstiver]

In @nature we describe the use of scalable #proteinengineering & #machinelearning to predict millions of bespoke CRISPR-Cas9 enzymes, offering safer & more genome editing tools. 🧬🖥️
@RachelSilvers11 @CGM_MGH @MGH_RI @MassGeneralNews @harvardmed
https://t.co/oNtG93uXCl

[Rachel Silverstein @RachelSilvers11]

Excited to share my PhD work… and more CRISPR-Cas9 PAM variants than anybody asked for

[Michael Kaminski Lab @KaminskiLab]

@BKleinstiver @Nature @RachelSilvers11 @CGM_MGH @MassGeneralNews @harvardmed Fantastic work. Congrats @BKleinstiver @RachelSilvers11 & team

[Michael Kaminski Lab @KaminskiLab]

@ChariteBerlin Base editing corrected the R2216W variant in primary cells, restoring polycystin-1 and reducing ER stress. In vivo correction reduced liver cysts, highlighting the potential of ADPKD reversibility & addressing extrarenal complications unmet by #Tolvaptan. (3/3)

[Michael Kaminski Lab @KaminskiLab]

Happy to share our new preprint on in vivo #CRISPR base editing in #ADPKD. Joint effort with Rishi Bhardwaj & Sorin Fedeles @YaleMed and Matteus Krappitz @CUAnschutz. Brief thread 👇 (1/3)
https://t.co/xKgKJWyizV

[Michael Kaminski Lab @KaminskiLab]

#ADPKD, the most common genetic kidney disorder, affects 10M+ peopleworldwide. Using #CRISPR base editing, we corrected pathogenic PKD1 variants from a @ChariteBerlin patient cohort with high efficiency in vitro. (2/3)

[Sarah Wendlinger @SarahWendlinger]

Thanks to everyone who came by our poster @carcor1608 for a chat at #esgct2024
Had a great time discussing our work and enjoyed Rome 🍝
@KaminskiLab

[Michael Kaminski Lab @KaminskiLab]

Thank you for a wonderful time in Rome @ESGCT! Lots of inspiring talks and great discussions at our posters. #esgct2024

[Michael Kaminski Lab @KaminskiLab]

Multi-analyte lateral flow enables visualization of genotypes at the point-of-care (5/5)

[Michael Kaminski Lab @KaminskiLab]

Excited to share our new @EmboMolMed paper on #CRISPR diagnostics for multiplexed point-of-care genotyping of #APOL1 variants. Many thanks to our collaborator @LVRiella @MGH. Brief thread 👇(1/5)

https://t.co/WuyfPWXE4K

[Michael Kaminski Lab @KaminskiLab]

Genotyping based on fluorescence readouts is reproducible at different clinicalcenters through normalization to a synthetic standard and a machine-learning-based analysis (4/5).