All life depends on accurate translation (both intracellular & international). Using time-resolved cryo-EM, we watched ribosomes decode mRNA, aided by EF-Tu and GTP. See our animations & article (https://t.co/mFffpChFRt) on how ribosomes proofread tRNAs to make correct proteins:
The Gonzalez Lab at Columbia University is seeking a scientific leader!
Join us in a Research Scientist position, driving single-molecule, structural, and biochemical studies of translation, translational control, and other RNA-based processes.
Apply: https://t.co/84JwVnrhS1
GTPBP1 is a GTPase implicated in neurodevelopment. Our cryo-EM maps show GTPBP1 delivering tRNA to ribosome. GTPBP1 promotes more stringent mRNA decoding than the bona fide delivery factor eEF1A.
Congrats to Denis Susorov and collab with Pestova lab! https://t.co/XaiYul04Zg
Using "in extracto cryo-EM", we visualize ribosomes in mammalian lysates, including RRL. "Hibernating" ribosomes carry proteins that protect functional centers. These include elongation factor eEF2, LARP1 implicated in mTOR signaling, eIF5A etc. https://t.co/gG2hM2G1Ax
Preprint from our collaboration with the labs of Gerry Wright and Shura Mankin @shuramankin identifying the first translation inhibitor binding to the E-site of the bacterial ribosome… https://t.co/gDSnWPSnsA
With our RTI colleagues Xiaobin Ling and @WenwenFang2, we report cryo-EM structures of enigmatic bacterial RNA nanocages made of long-noncoding ROOL RNAs. Octamers are held by "A-minor staples" and other interactions. Congrats Xiaobin, Dima et al!
https://t.co/kThZa5hsMS
“Undermining the future of American science is not a path to accountability — it’s a path to decline.”
In a powerful new piece, my colleague Daphna Shohamy explains how canceled grants are driving young scientists out of science—and out of the country.
🔗 https://t.co/nlZKwjar3L
In our newly published study we show that the proline-rich antimicrobial peptide (PrAMP) Bac5 has a distinct mode of interaction with the ribosome compared to other PrAMPs…https://t.co/AECODPspzg
We developed a new siRNA drug candidate for prion disease, a @broadinstitute / @RTI_UMassChan collaboration. We now have an open IND meaning FDA has given us permission to start first-in-human trials. Work remains before trials can launch. The full update: https://t.co/tmL2vIZT4z
I’m happy to share our recent manuscript in which we are structurally and biochemically investigating 3'-5' tRNA splicing ligases, including a #cryoEM structure of the five-subunit D. rerio tRNA ligase complex.
https://t.co/VtfbOrx5u5
Fresh off the press…our latest study on the Structure of a Gcn2 dimer in complex with the large 60S ribosomal subunit | great collaboration with @inada_lab and Zaher lab. Data collected at @DCI_EM.
PNAS https://t.co/qnGrbmG5ud
Biological systems find ingenuous solutions. Almost anything that can happen, happens.
Still, I would not have guessed a role of RNA-binding proteins on the cell surface.
The Flynn lab are boldly investigating glycoRNAs and following their leads.
Our paper on Stopless-ORF Circular RNAs (socRNAs) is now out in Cell. By high-res tracking and comparing translation by either single or multiple ribosomes, we find that ribosomes cooperate to overcome pausing to ensure fast and efficient translation
https://t.co/Oyw32LlbxM
Our review on small endogenous RNAs in animals is online. We thank @MartinjSimard, @SvobodaLab and the third, anonymous, reviewer for helping us improve our manuscript. https://t.co/T0cDfVIGpg