@JohannesPilic My subjective perception of scientific success... A little hard to measure accurately๐ My postdoctoral lab running out of money before I published anything was hard
Unrelated: last week I gave a seminar for the students of the Science program of Charles University (https://t.co/xSoFj9ioeq). Seems like an excellent program and a lovely group of students!๐๐ I hope they enjoyed it as much as I did!
I took my hand (specifically, my hurting middle finger) to the doctor today... Here is the result๐. They made me do it๐. Less fun: if you look closely, you will see that the end of the bone is crushed๐
Got my hand pinched by a door a week ago... Ouch! ๐ฐ One of my fingers still hurts, but Petro has now at least made it look better... At least in Photoshop๐
Check out how this translocation-based biosensor develops linear dichroism (when the orientational freedom of the fluorescent protein becomes restricted)! ๐ Imaged with our new, super-fast confocal LD microscope๐ฌโก๏ธ, now available from https://t.co/rvRYJcEXny
We used a super-fast linear dichroism confocal microscope based developed in collaboration with our friends at KU Leuven:
The TriScan: fast and sensitive 3D confocal fluorescence imaging using a simple optical design https://t.co/5TFAVTTQfF
Microscopy imaging of activation of the b2-adrenergic receptor by using a FLIP biosensor - resting state (on the left) and after b2AR activation (on the right). Can you spot the difference?๐ค Pretty cool, huh? ๐๐
FLIPs are new, super-simple molecular biosensors that convert molecular events into changes in orientation of a fluorescent protein. This change is then observable by linear dichroism (LD) microscopy. Isn't this simple?
And this is the paper we were celebrating!๐ฅ:
FLIPs: Genetically encoded molecular biosensors for functional imaging of cell signaling by linear dichroism microscopy | Science Advances https://t.co/gNca19SVnc
We had a little celebration of our upcoming paper in Science Advances... One of the co-authors showed up in full strength!๐ช๐ฑ Keep up the good work, Alex!๐
We see that an intrabody based on NB32 distinguishes between different GPCR conformations. This can be used in biosensors based on bioluminescence, but also in fluorescent biosensors that utilize linear dichroism/polarization microscopy๐ฌ. Check it out!
A joint manuscript with @arshukla accepted, with Vendy Nagy Markovรก as a first co-author! Thank you, Arun & al.! Btw., first submission: 1.5 years ago ๐
Got back from the EMC meeting in Copenhagen @eurmicsoc#EMC2024... Impressive results by
@CizmarTomas, and a well-deserved prize, for imaging through an optical fiber๐โโ๏ธ๐งต๐๐ฅ๐ผ๏ธ!
Also impressive: the Copenhagen airport security!๐ฎ๐
Another summer visitor to the lab: Mikel Garcia-Marcos (@MGMlab) from Boston U. stayed for a whole month, and even came to our lab retreat!๐๏ธ๐น๐ฅค๐บ ๐ฒ๐ญExcellent exchange of know-how! Curious to see what comes out of it! ๐ก๐ฌ
Finally: we are now revising our joint paper with Arun Shukla (@arshukla) on cool conformation biosensors of arrestin signaling:
https://t.co/WoYp5iJDCs
Looking forward to getting it published!