Neuronal Signalling Lab

Neuronal Signalling Lab @BioscienceTurku - focused on increasing throughput of optical methods to study signalling in disease.

[Neuronal Signalling Lab @MJC_lab]

@DrLachie We have data going to different server shares, depending on the HT imager and user account i.e. some grouping of users to single shares. It was quick to set up - maybe a quick way to free up HD space? But then we don't have that so many hands-on users ... yet...

[Neuronal Signalling Lab @MJC_lab]

@joachimgoedhart @AbellJonny @christlet @NathanShaner I think we were seeing nano3 ~7 x brighter (than nano) in our preliminary tests a while back, but it also rather sensitive to what's at the C-terminus. We wanted to have an NLS version and, for nano3 at least, GS linkers were critical.

[Advanced Bioimaging Center @ UC Berkeley @ABCUCBerkeley]

We are excited to introduce PetaKit5D, a comprehensive suite of software tools designed to tackle challenges in the handling of large datasets and make processing of petabyte-scale datasets feasible: https://t.co/P2WaWODV3F

[biophotonicat @biophotonicat]

Image processing tools for petabyte-scale light sheet microscopy data (relevance: 61%) https://t.co/xhUZinyBLP #biophotonics #biomedicaloptics

[Neuronal Signalling Lab @MJC_lab]

A FRET reporter of PTEN activity, used to study pathogenic missense-induced loss of function and dynamic regulation in vivo...

[Tal Laviv @LavivTal]

Very excited to share the first preprint from my lab!
We have developed a biosensor which allows to monitor PTEN activity in living cells, tissue and even whole (small) organisms!!
https://t.co/OhhWeoMoDL

[Alfred Millett-Sikking @amsikking]

This corresponds closely with the O2-O3 'cross brace' solution from @JLazzariDean. Based on the above data, reversing one of the POLARIS-K2T mounts will neutralize their thermal effect, leaving just the aluminum cross brace and an ~exact cancelation:
https://t.co/yULf8o71Pk

[Takeharu Nagai @Lab_Nagai]

Our paper regarding ”spontaneous" molticolor bioluminescence imaging was published at PNAS. This technology can be applied to wide range of living supecimen including bacteria, animal and plant. Addition of a luminescent substrate is no longer required.　https://t.co/lMQSGdqujG

[Alfred Millett-Sikking @amsikking]

Thermal expansion -> a typical problem in time-lapse microscopy at high resolution. Here I measure the axial shift of the focal plane of a Nikon 40x0.95 air objective at ~1.6um/K, or about ~1.5x the depth of field per degree C:
https://t.co/ZafijhH7AX

[Neuronal Signalling Lab @MJC_lab]

As well as providing experimental insight into cellular/molecular functionality in a neuronal environment of some SynGAP1 variants currently categorised variants of uncertain significance, it will help indicate which assays would be most suitable for future compound screens. 2/2

[Neuronal Signalling Lab @MJC_lab]

Thanks to SRF @cureSYNGAP1 for funding our new project on SynGAP1 missense.
With coPI @LILILI_LLL, our team will expand our SynGAP1 missense AAV panel, compare 24 variants +/- 3 different repurposing candidates with selected assays developed in our earlier SRF/Leon&friends 1/2

[Neuronal Signalling Lab @MJC_lab]

and best of luck to @ANeurosci on your latest move

[Neuronal Signalling Lab @MJC_lab]

(belated) congratulations to @ANeurosci and all other authors of this paper! Delighted that our team could contribute.
The paper includes the first published use of @LILILI_LLL’s minaturised synaptoneurosome procedure for synaptic proteomics…

[Anna Y. Yotova @ANeurosci]

📢Beyond thrilled to see my 1st fist-author paper in @BrainBehavImm @PNIRS🎉

This effort was co-supervised/conseptualised by @ffreudenberg & @daslattery w/ the invaluable input from @AetOleary, @LabReif, @MJC_lab & @LILILI_LLL

🔗https://t.co/yHaoPLZSLy

🧵on my #PhD magnum opus

[Alfred Millett-Sikking @amsikking]

#AIRR microscopy, the final touch: Real-time multi-angle projections!
-> Get ultra-fast 2D projections of 3D objects of any refractive index, with a light-sheet microscope that uses a single AIR objective at the sample! Crazy!

[CURE SYNGAP1 aka SynGAP Research Fund 501(C)(3) @cureSYNGAP1]

June is #SyngapAwarenessMonth! SRF helps all families living with #SYNGAP1, but don't take our word for it...

Hear from our volunteers - https://t.co/FO9bWEMvOj
Learn from our resources - https://t.co/YAzZULAjtp

#Splash4Syngap #CureSyngap1 #Community

[Greg Bowman @drGregBowman]

Ever wonder if kinetics are really important, or if you just need thermodynamics?

Here's a great example of where kinetics are key!

We show that accounting for dynamics that occur during single molecule FRET experiments (which we call time-averaging) is often critical for getting good agreement between simulations and experiments, and for arriving at correct structural insights from both sources of information.
https://t.co/hmLHtcRMFX

[Joachim Goedhart @joachimgoedhart]

TurCaMP: bright cyan fluorescence calcium indicator for mitochondrial calcium with minimal interference from physiological pH fluctuations
https://t.co/dn5z2Sm0Jt

[Neuronal Signalling Lab @MJC_lab]

Magnetogenetics - a magnetic field-sensitive LOV2, with > 75% dynamic range by fluorescence.

[Andrew York @AndrewGYork]

Meet MagLOV: an engineered protein that responds STRONGLY to magnetic fields.

This is a fluorescence timelapse of MagLOV in E. coli. We're waving a (small) magnet under the plate.

Can you tell where the magnet is?

Want some? It's on Addgene now! https://t.co/eWs0aAIT1A

[Bose-Einstein-Kondensat @MWNautilus]

#OpenSource mod of a desktop #FFF printer via 980 nm #NIR #laser addition for upconversion #3Dprinting:

-penetration depths up to 5.8 cm
-printing inside/through existing #3Dprinted parts
-in situ #bioprinting

https://t.co/v1xWkGEXqP
#stereolithography #DIYbio #lab #instruments

[InSiDESignalingDynamics @InSiDE_Seminar]

‼️DEADLINE EXTENDED🗓️--Abstracts for in-person InSiDE are now due May 26th. Be sure to register now and get your abstracts submitted for consideration for poster presentations and short talks!