Just out โก๏ธ @WHO Global #TB Report 2020 with the latest data on #TB burden & response.
Urgent action & investment is needed to #EndTB, especially as #COVID19 threatens to unwind gains made in the #EndTB fight. #SaveLives https://t.co/9AOYI3t5a6
...don't miss out the recent publication (4 weeks ago) about mycobactins of M. marinum (PMID 31945239). If you are interested in iron utilisation by mycobacteria, read these recent publications: PMID 32069330 & 31108902.
Image taken from 31945239 (part of Fig.1).
#lipids#Mtb
Let's talk about #Mycobactin. These lipids/siderophores are important for extra- & intracellular growth of #mycobacteria, as they scavenge iron. There are lipid-bound mycobactins & H2O-soluble carboxymycobactins. Read about their Biosynthesis Pathway here: PMID 22232695. Also,...
Don't miss out this paper (3 weeks old), where they analysed Mycolic Acids (ฮฑ-, keto- , carboxy- & wax ester mycolates) from Mycobacterium avium via NMR and MALDI-MS.
https://t.co/PeMklw2079
#lipids#mycobacterium#massspec
...derived side chain. The length of both the backbone and side chain can vary. Photo taken from PMID 26818253. Here is a Mass Spec paper about PDIM: ๐26574042. Overexpression of PDIM was also shown to reprogram #macrophage metabolism ๐ 30327492.
#lipids#lipidomics#Mtb
The next two lipid classes of interest are the phthiocerol dimycocerosates (#PDIM) & phenolic glyolipids (#PGL). The PDIM backbone consists of a phthiocerol-derived moiety, whereas the PGL backbone has a phenolphthiocerol-derived moiety. Also, both contain a mycocerosic acid-...
...reviews if you want to know more about their structure, function and involved enzymes (pathway): PMID 30775376, 29722821, 26586646, 22191451 and 21521247.
#lipids#lipidomics#cellenvelope#mycobacterium
#Lipoarabinomannan (LAM) is the next (glyco)lipid on our list. It is synthesised via PI ๐ PIM ๐ AcPIM1-6 ๐ LM ๐LAM. They all play important roles in the pathogenesis of #Mtb & exhibit distinct immunomodulatory properties. They could also function as biomarker. Check these...
...ฮฑ-Mycolates, Methoxy-Mycolates, Keto-Mycolates and Hydroxy-Mycolates with Cis/Trans variations. Interested in details? Have a look here: PMID 24374164, 27268593, 31214994, 31267927 and https://t.co/ha7zzKOTUD
#lipids#Mtb#mycobacterium
Trehalose Dimycolate (cord factor; trehalose esterified to two ฮฑ-alkyl, ฮฒ-hydroxy FA of varying length) is the best known mycobacterial lipid. The mycolic acid moiety can have cyclopropane rings, double bonds, and methylations, making it structurally very divers. There are...
If you are interested in extracting the #lipidome, this review is a must read: 'Exploring the Lipidome: Current Lipid Extraction Techniques for Mass Spectrometry Analysis'
https://t.co/BPD4Tp6D5i
#lipidomics#massspectrometry
Here is another good review about 'Cell Walls and Membranes of Actinobacteria'. It includes #lipids of #mycobacteria and #corynebacteria. https://t.co/58LhEi8cpm
The mycobacterial cell envelope is very complex. It contains e.g. phosphatidylinositolmannosides (PIM), mycolic acids (MA), trehalose mycolates (TMM, TDM), phthioceranic acids, phthiocerol dimycocerosates (PDIMA/B) and mycobactins. We will talk about them all. #lipidomics#lipid
Helpful databases and tools to analyse Mycobacterial #lipids are e.g. MtbLipidDB, MycoMass, MycoMaps and LipidMaps. What is your favourite one?
#mycobacterium#TB
In 2018, 5 % of all people diagnosed with TB were infected with a drug-resistant strain. And many TB drugs target the cell envelope, in particular #lipids. That's why #lipidomics of #Mycobacteria is so important.