Mycobacterial Lipidomics

MS-based mycobacterial #Lipidomics is urgently needed to tackle antimicrobial resistance. #lipids #massspectrometry #tuberculosis #membrane #AMR #mycobacteria

[Mycobacterial Lipidomics @MLipidomics]

Don't miss out this update 👇 #tuberculosis #WHO

[Marie-Christine Bartens @marie_bartens]

Just out ➡️ @WHO Global #TB Report 2020 with the latest data on #TB burden & response.
Urgent action & investment is needed to #EndTB, especially as #COVID19 threatens to unwind gains made in the #EndTB fight. #SaveLives https://t.co/9AOYI3t5a6

[Mycobacterial Lipidomics @MLipidomics]

...don't miss out the recent publication (4 weeks ago) about mycobactins of M. marinum (PMID 31945239). If you are interested in iron utilisation by mycobacteria, read these recent publications: PMID 32069330 & 31108902.
Image taken from 31945239 (part of Fig.1).
#lipids #Mtb

[Mycobacterial Lipidomics @MLipidomics]

Let's talk about #Mycobactin. These lipids/siderophores are important for extra- & intracellular growth of #mycobacteria, as they scavenge iron. There are lipid-bound mycobactins & H2O-soluble carboxymycobactins. Read about their Biosynthesis Pathway here: PMID 22232695. Also,...

[Mycobacterial Lipidomics @MLipidomics]

Don't miss out this paper (3 weeks old), where they analysed Mycolic Acids (α-, keto- , carboxy- & wax ester mycolates) from Mycobacterium avium via NMR and MALDI-MS.
https://t.co/PeMklw2079
#lipids #mycobacterium #massspec

[Mycobacterial Lipidomics @MLipidomics]

...derived side chain. The length of both the backbone and side chain can vary. Photo taken from PMID 26818253. Here is a Mass Spec paper about PDIM: 👉26574042. Overexpression of PDIM was also shown to reprogram #macrophage metabolism 👉 30327492.
#lipids #lipidomics #Mtb

[Mycobacterial Lipidomics @MLipidomics]

The next two lipid classes of interest are the phthiocerol dimycocerosates (#PDIM) & phenolic glyolipids (#PGL). The PDIM backbone consists of a phthiocerol-derived moiety, whereas the PGL backbone has a phenolphthiocerol-derived moiety. Also, both contain a mycocerosic acid-...

[Mycobacterial Lipidomics @MLipidomics]

...reviews if you want to know more about their structure, function and involved enzymes (pathway): PMID 30775376, 29722821, 26586646, 22191451 and 21521247.
#lipids #lipidomics #cellenvelope #mycobacterium

[Mycobacterial Lipidomics @MLipidomics]

#Lipoarabinomannan (LAM) is the next (glyco)lipid on our list. It is synthesised via PI 👉 PIM 👉 AcPIM1-6 👉 LM 👉LAM. They all play important roles in the pathogenesis of #Mtb & exhibit distinct immunomodulatory properties. They could also function as biomarker. Check these...

[Mycobacterial Lipidomics @MLipidomics]

...α-Mycolates, Methoxy-Mycolates, Keto-Mycolates and Hydroxy-Mycolates with Cis/Trans variations. Interested in details? Have a look here: PMID 24374164, 27268593, 31214994, 31267927 and https://t.co/ha7zzKOTUD
#lipids #Mtb #mycobacterium

[Mycobacterial Lipidomics @MLipidomics]

Trehalose Dimycolate (cord factor; trehalose esterified to two α-alkyl, β-hydroxy FA of varying length) is the best known mycobacterial lipid. The mycolic acid moiety can have cyclopropane rings, double bonds, and methylations, making it structurally very divers. There are...

[Mycobacterial Lipidomics @MLipidomics]

Polyketides are also listed

[Stephan Klatt @KlattStephan]

If you are interested in extracting the #lipidome, this review is a must read: 'Exploring the Lipidome: Current Lipid Extraction Techniques for Mass Spectrometry Analysis'
https://t.co/BPD4Tp6D5i
#lipidomics #massspectrometry

[Mycobacterial Lipidomics @MLipidomics]

Here is another good review about 'Cell Walls and Membranes of Actinobacteria'. It includes #lipids of #mycobacteria and #corynebacteria. https://t.co/58LhEi8cpm

[Mycobacterial Lipidomics @MLipidomics]

The mycobacterial cell envelope is very complex. It contains e.g. phosphatidylinositolmannosides (PIM), mycolic acids (MA), trehalose mycolates (TMM, TDM), phthioceranic acids, phthiocerol dimycocerosates (PDIMA/B) and mycobactins. We will talk about them all. #lipidomics #lipid

[Mycobacterial Lipidomics @MLipidomics]

Here is a nice review about the metabolism of the cell wall and surface #lipids in M. tuberculosis during growth and stasis: https://t.co/y09cGmkIFg

[Mycobacterial Lipidomics @MLipidomics]

This workbench is a must for every #Lipidomics geek. #LipidCreator

[Mycobacterial Lipidomics @MLipidomics]

MS-DIAL with LipidBlast and Skyline are very helpful tools to analyse Mass Spec data. They are also freeware. #LCMS #lipids

[Mycobacterial Lipidomics @MLipidomics]

Helpful databases and tools to analyse Mycobacterial #lipids are e.g. MtbLipidDB, MycoMass, MycoMaps and LipidMaps. What is your favourite one?
#mycobacterium #TB

[Mycobacterial Lipidomics @MLipidomics]

In 2018, 5 % of all people diagnosed with TB were infected with a drug-resistant strain. And many TB drugs target the cell envelope, in particular #lipids. That's why #lipidomics of #Mycobacteria is so important.